Classical and non-classical Major Histocompatibility Complex class I gene expression in in vitro derived bovine embryos

Doyle, Julie; Ellis, Shirley A.; O'Gorman, Grace M.; Donoso, Ines Maria Aparicio; Lonergan, Patrick; Fair, Trudee

doi:10.1016/j.jri.2009.06.125

Cited by 23 publications

(22 citation statements)

References 35 publications

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“…We have recently described the mRNA expression pattern of NC1 during preimplantation embryo development in cattle and found the highest levels of expression in metaphase II stage oocytes and presumptive zygotes [27]. Additionally, generic primers, which were compatible with classical MHC-I and NC1 alleles, detected 10% of blastocyst cDNA sequences as NC1 [27].…”

Section: Introductionmentioning

confidence: 98%

Regulation of a Bovine Nonclassical Major Histocompatibility Complex Class I Gene Promoter1

O'Gorman

Naib²,

Ellis

et al. 2010

Biology of Reproduction

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Studies have shown in humans and other species that the major histocompatibility complex class I (MHC-I) region is involved at a number of levels in the establishment and maintenance of pregnancy. The aim of this study was to characterize how a bovine nonclassical MHC-I gene (NC1) is regulated. Initial serial deletion experiments of a 2-kb fragment of the NC1 promoter identified regions with positive regulatory elements in the proximal promoter and evidence for a silencer module(s) further upstream that cooperatively contributed to constitutive NC1 expression. The cytokines interferon tau (IFNT), interferon gamma (IFNG), and interleukin 4 (IL4) significantly increased luciferase expression in NC1 promoter reporter constructs and endogenous NC1 mRNA levels in a bovine endometrial cell line. In addition, IFNG, IL3, IL4, and progesterone significantly increased Day 7 bovine blastocyst NC1 mRNA expression when supplemented during in vitro embryo culture. Site-directed mutagenesis analysis identified a STAT6 binding site that conferred IL4 responsiveness in the NC1 proximal promoter. Furthermore, methylation treatment of the proximal promoter, which contains a CpG island, completely abrogated constitutive NC1 expression. Overall, the findings presented here suggest that constitutive NC1 expression is regulated positively by elements in the proximal promoter, which are further controlled by upstream silencer modules. The promoter is responsive to IFNT, IFNG, and IL4, suggesting possible roles for these cytokines in bovine preimplantation embryo survival and/or maternal-fetal tolerance. Our studies also suggest that methylation of the proximal promoter, in particular, could play a significant role in regulating NC1 expression.

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Section: Introductionmentioning

confidence: 98%

Regulation of a Bovine Nonclassical Major Histocompatibility Complex Class I Gene Promoter1

O'Gorman

Naib²,

Ellis

et al. 2010

Biology of Reproduction

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“…There were two classes of major histocompatibility complex (MHC) antigens, whereas, only MHC class I antigens were expressed on the surface of the conceptus. MHC class I molecules or transcripts could be detected throughout development to the blastocyst stage in cow404142. However, at the period of placental attachment, little MHC antigens were expressed.…”

Section: Discussionmentioning

confidence: 99%

Deep sequencing of transcriptome profiling of GSTM2 knock-down in swine testis cells

Jin

Zhou

et al. 2016

Sci Rep

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Glutathione-S-transferases mu 2 (GSTM2), a kind of important Phase II antioxidant enzyme of eukaryotes, is degraded by nonsense mediated mRNA decay due to a C27T substitution in the fifth exon of pigs. As a reproductive performance-related gene, GSTM2 is involved in embryo implantation, whereas, functional deficiency of GSTM2 induces pre- or post-natal death in piglets potentially. To have some insight into the role of GSTM2 in embryo development, high throughput RNA sequencing is performed using the swine testis cells (ST) with the deletion of GSTM2. Some embryo development-related genes are observed from a total of 242 differentially expressed genes, including STAT1, SRC, IL-8, DUSP family, CCL family and integrin family. GSTM2 affects expression of SRC, OPN, and SLCs. GSTM2 suppresses phosphorylation of STAT1 by binding to STAT1. In addition, as an important transcription factor, STAT1 regulates expression of uterus receptive-related genes including CCLs, IRF9, IFITs, MXs, and OAS. The present study provides evidence to molecular mechanism of GSTM2 modulating embryo development.

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“…The existence and genomic location on chromosome 23, of five bovine MHC-Ib genes (named NC1-NC5), is recorded on the database. Their expression has been demonstrated in early cleavage stage bovine embryos (Doyle et al, 2009) binucleate cells (Bainbridge et al, 2001) and in first and second trimester and term trophoblast tissues (Davies et al, 2006). In general, the classical class I genes are found to be down-regulated or modified in the trophoblast cell populations in many mammalian species (for review, see Ellis et al, 2004).…”

Section: Molecular Response Of Maternal Endometrium To the Embryomentioning

confidence: 99%

“…In general, the classical class I genes are found to be down-regulated or modified in the trophoblast cell populations in many mammalian species (for review, see Ellis et al, 2004). MHC-I mRNA expression by bovine embryos is both transcript and embryo stage-specific (Doyle et al, 2009) and can be regulated by a number of cytokines including IFNG, IL-4, and LIF (O'Gorman et al, 2010;Al Naib et al, 2011). Humans express three classical class I genes (HLA-A, -B and -C), and a number of non-classical genes, including HLA-G. HLA-G is expressed by human trophoblast (Ellis et al, 1999), which exists in both membrane-bound and soluble (secreted) alternatively spliced forms.…”

Section: Molecular Response Of Maternal Endometrium To the Embryomentioning

confidence: 99%

“…However, these roles may be more important for mouse and human, where implantation is quite invasive. An additional role, which may be more relevant to ruminant species, is regulating the activation of anti-conceptus immune responses (Oliveira et al, 2010) in response to IFNT stimulation and antigenicity of the conceptus due to paternal antigen and classical MHC protein expression (Doyle et al, 2009). In cattle, using immunofluorescent labeling of immune cell markers, we observed an initial expansion of Mo, MØs (CD14+-cells), and DC (CD172a-CD11c+) populations in the endometrial stroma on day 13 of pregnancy (Mansouri-Attia et al, 2012).…”

Section: Maternal Immune Cell Response To Pregnancymentioning

confidence: 99%

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Embryo maternal immune interactions in cattle

Fair¹

2016

Anim Reprod

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Mammalian embryo implantation requires the priming of the maternal immune system, but, not the provocation. There are many examples of conditions where a disturbed or aberrant immune profile during embryo implantation leads to pregnancy loss. However, these studies are primarily associated with human and mouse species; data is generally limited for cattle and livestock. Most available information centres on the endometrial response to interferon tau (IFNT), a type I antiviral cytokine, which is the maternal recognition factor for cattle and sheep. Interferon tau secretion by the embryo and detection by the dam is critical to corpus luteum (CL) maintenance and pregnancy retention. However, the large volume of bovine endometrial and conceptual transcriptomic data highlights a broader more integral role of the maternal immune system in the establishment of pregnancy in cattle. When taken together with available immunohistochemistry and flow cytometry data from livestock, mouse, and human, a profile of immune cell involvement from ovulation to conception and placentation emerges. The key events of pregnancy establishment in cattle and the involvement of the maternal immune system will be discussed.

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Classical and non-classical Major Histocompatibility Complex class I gene expression in in vitro derived bovine embryos

Cited by 23 publications

References 35 publications

Regulation of a Bovine Nonclassical Major Histocompatibility Complex Class I Gene Promoter1

Regulation of a Bovine Nonclassical Major Histocompatibility Complex Class I Gene Promoter1

Deep sequencing of transcriptome profiling of GSTM2 knock-down in swine testis cells

Embryo maternal immune interactions in cattle

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