Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

Fatokun, Evelyn Nwadinkpa; Nwodo, Uchechukwu U.; Okoh, Anthony I.

doi:10.3390/app6100286

Cited by 55 publications

(27 citation statements)

References 59 publications

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“…In addition, many enzymatic processes and the transport of various components across the cell membrane are strongly affected by the pH of the medium [55]. A optimum pH is required to maintain the three-dimensional shape of the active site of enzymes and the change in pH results in a loss of functional shape of the enzyme due to alterations in the ionic bonding of the enzyme.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

et al. 2016

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This paper reports on the optimization of culture conditions for cellulase and xylanase production by bacterial isolate from lignocellulosic biomass. The bacterial isolate was screened for cellulase and xylanase production on carboxyl methyl cellulose (CMC) and birch wood xylan as substrates, respectively. One bacterial isolate showing the highest halo zone diameter (isolate PLY1) was selected for detailed studies. The analysis of the 16S ribosomal ribonucleic acid (rRNA) gene nucleotide sequence of PLY1 revealed it to have 98% similarity to Micrococcus luteus strain Fse9 and the sequence was deposited in the GenBank as Micrococcus luteus strain SAMRC-UFH3 with accession number KU171371. Cellulase production was achieved in the presence of CMC (1% w/v) under an incubation temperature of 25 • C (198 U/mL), pH 5 (173 U/mL), agitation speed 50 rpm (173 U/mL) and incubation period of 96 h (102 U/mL). Xylanase was produced maximally when birch wood xylan (1% w/v) was used as the substrate at 25 • C (1007 U/mL), pH 10 (2487 U/mL), 200 rpm (1814 U/mL), and under an incubation period of 84 h (1296 U/mL). Our findings showed that Micrococcus sp. SAMRC-UFH3 appears to be a potentially important candidate for lignocellulosic waste degradation and other relevant industrial applications.

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Section: Discussionmentioning

confidence: 99%

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

et al. 2016

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“…The parameters for optimal extracellular peroxidase activity were determined as described by Fatokun et al. . Briefly, the various culture parameters were adjusted for optimum peroxidase activity using the conventional method of one factor at a time.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, the various culture parameters were adjusted for optimum peroxidase activity using the conventional method of one factor at a time. Then, the determined optimized conditions were used in subsequent fermentation .…”

Section: Methodsmentioning

confidence: 99%

Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity

Falade

Mabinya

Okoh

et al. 2018

Biotech and App Biochem

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The industrial applications and prospects of microbial peroxidase are on the upwards trend, thus necessitating the search for sources with high turnaround time. Actinobacterial species have been a major source of peroxidase for the obvious reasons of having robust metabolite expression capabilities. However, other bacteria species have been underexplored for peroxidase production, hence the motivation for the investigation into the peroxidase production potential of Raoultella ornithinolytica OKOH-1 (KX640917). The bacteria expressed optimum specific peroxidase activity of 16.48 ± 0.89 U mg −1 , which is higher than those previously reported. The optimal fermentation conditions were pH 5 (3.44 ± 0.64 U mL −1 ), incubation temperature of 35 • C (5.25 ± 0.00 U mL −1 ), and agitation speed of 150 rpm (9.45 ± 2.57 U mL −1 ), with guaiacol and ammonium chloride as the best inducer and nitrogen supplement, respectively. On valorization of agrowastes as a sole carbon source for the secretion of peroxidase, sawdust gave the best peroxidase yield (15.21 ± 2.48 U mg −1 ) under solid-state fermentation. Also, a nonperoxide-dependent enzyme activity, which suggests probable laccase activity, was observed. The ability of the bacteria to utilize agrowastes is highly economical and as well a suitable waste management strategy. Consequently, R. ornithinolytica OKOH-1 is a promising industrial strain with dexterity for enhanced peroxidase production.

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“…NZ produced maximum enzyme titre near the end of the exponential phase and early stationary phase [65]. B. cereus [66] and B. halodurans PPKS-2 [67] showed maximum xylanase production after 48 h. Gupta and Kar [68] studied xylanase production by Bacillus sp. and reported that maximum xylanase production was observed in 48 h and 72 h using wheat bran and corn cob as a substrate, respectively.…”

Section: Resultsmentioning

confidence: 99%

Endoglucanase and Xylanase Production by Chryseobacterium Species Isolated from Decaying Biomass

Nkohla¹,

Okaiyeto²,

Nwodo³

et al. 2017

Pol. J. Environ. Stud.

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Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

Cited by 55 publications

References 59 publications

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity

Endoglucanase and Xylanase Production by Chryseobacterium Species Isolated from Decaying Biomass

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