Classical swine fever virus isolates from Cuba form a new subgenotype 1.4

Postel, Alexander; Schmeiser, Stefanie; Perera, Carmen Laura; Rodríguez, Lester Josué Pérez; Frías‐Lepoureau, María Teresa; Becher, Paul

doi:10.1016/j.vetmic.2012.07.045

Cited by 83 publications

(65 citation statements)

References 11 publications

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“…The genotypes of CSF viruses can be classified into three major groups with eleven subgroups [3–5]. Group 1 (1.1, 1.2, 1.3, & 1.4) contains primarily historical strains isolated from many regions of the world and includes all live-attenuated vaccine strains.…”

Section: Introductionmentioning

confidence: 99%

Pigs immunized with a novel E2 subunit vaccine are protected from subgenotype heterologous classical swine fever virus challenge

et al. 2016

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BackgroundClassical swine fever (CSF) or hog cholera is a highly contagious swine viral disease. CSF endemic countries have to use routine vaccination with modified live virus (MLV) vaccines to prevent and control CSF. However, it is impossible to serologically differentiate MLV vaccinated pigs from those infected with CSF virus (CSFV). The aim of this study is to develop a one-dose E2-subunit vaccine that can provide protection against CSFV challenge. We hypothesize that a vaccine consisting of a suitable adjuvant and recombinant E2 with natural conformation may induce a similar level of protection as the MLV vaccine.ResultsOur experimental vaccine KNB-E2 was formulated with the recombinant E2 protein (Genotype 1.1) expressed by insect cells and an oil-in-water emulsion based adjuvant. 10 pigs (3 weeks old, 5 pigs/group) were immunized intramuscularly with one dose or two doses (3 weeks apart) KNB-E2, and 10 more control pigs were administered normal saline solution only. Two weeks after the second vaccination, all KNB-E2 vaccinated pigs and 5 control pigs were challenged with 5 × 105 TCID50 CSFV Honduras/1997 (Genotype 1.3, 1 ml intramuscular, 1 ml intranasal). It was found that while control pigs infected with CSFV stopped growing and developed high fever (>40 °C), high level CSFV load in blood and nasal fluid, and severe leukopenia 3–14 days post challenge, all KNB-E2 vaccinated pigs continued to grow as control pigs without CSFV exposure, did not show any fever, had low or undetectable level of CSFV in blood and nasal fluid. At the time of CSFV challenge, only pigs immunized with KNB-E2 developed high levels of E2-specific antibodies and anti-CSFV neutralizing antibodies.ConclusionsOur studies provide direct evidence that pigs immunized with one dose KNB-E2 can be protected clinically from CSFV challenge. This protection is likely mediated by high levels of E2-specific and anti-CSFV neutralizing antibodies.

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Section: Introductionmentioning

confidence: 99%

Pigs immunized with a novel E2 subunit vaccine are protected from subgenotype heterologous classical swine fever virus challenge

et al. 2016

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“…In Cuba, CSF is endemic [30], this is one of the most devastating diseases in swine, concerning both economic and sanitary issues [31]. In a previous study, in which identification of risk factors contributing to CSF occurrence in Bulgaria was one of the objective, human demography variables, such as population by municipality, place of residence (urban, rural) and gender, were analyzed.…”

Section: Resultsmentioning

confidence: 99%

Association between the swine production areas and the human population in Pinar del Río province, Cuba

Fonseca¹,

Santoro²,

Alfonso³

et al. 2017

Int J One Health

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Association between the swine production areas and the human population in Pinar del Río province, Cuba. Int J One Health 2017;3:36-41. AbstractAim: The aim of this study was to demonstrate the association between high human population density and high pig production in the province of Pinar del Río, Cuba. Materials and Methods:Records on pig movements at the district level in Pinar del Río province from July 2010 to December 2012 were used in the study. A network analysis was carried out considering districts, as nodes, and movements of pigs between them represented the edges. The in-degree parameter was calculated using R 3.1.3 software. Graphical representation of the network was done with Gephi 0.8.2, and ArcGIS 10.2. was used for the spatial analysis to detect clusters by the Getis-Ord Gi* method and visualize maps as well. Results: Significant spatial clusters of high values (hot spots) and low values (cold spots) of in-degree were identified.A cluster of high values was located in the central area of the province, and a cluster of low values involving municipalities of the Western zone was detected. Logistic regression demonstrated that a higher human population density per district was associated (odds ratio=16.020, 95% confidence interval: 1.692-151.682, p=0.016) with areas of high pork production. Conclusion:Hot spot of swine production in Pinar del Río is associated with human densely populated districts, which may suppose a risk of spillover of pathogens able to infect animals and humans. These results can be considered in strategy planning in terms of pork production increases and improvements of sanitary, commercial, and economic policies by decision-makers.

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“…The CSFV genome is a positive single-stranded RNA consisting of untranslated regions at both termini, the 5′-nontranslated region (5′-NTR) and 3′NTR, and a single large open reading frame (ORF) that was translated into a 3,898-amino-acid polyprotein and subsequently cleaved by viral and host proteases to generate four structural (Core, E rns , E1, and E2) and eight nonstructural (N pro , P7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B) proteins (1). Based on the nucleotide sequences of partial 5′NTR and NS5B and partial or whole E2, CSFV isolates have been divided into three genotypes and 11 subgenotypes (1.1 to 1.4, 2.1 to 2.3, and 3.1 to 3.4) (2–4). In mainland China, CSFV isolates circulating in the late 1980s and 1990s consisted of four subgenotypes (1.1, 2.1, 2.2, and 2.3) (5).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of a Sub-Subgenotype 2.1i Isolate of Classical Swine Fever Virus from China

Zhang

Bao

et al. 2017

Genome Announc

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Classical swine fever virus isolates from Cuba form a new subgenotype 1.4

Cited by 83 publications

References 11 publications

Pigs immunized with a novel E2 subunit vaccine are protected from subgenotype heterologous classical swine fever virus challenge

Pigs immunized with a novel E2 subunit vaccine are protected from subgenotype heterologous classical swine fever virus challenge

Association between the swine production areas and the human population in Pinar del Río province, Cuba

Complete Genome Sequence of a Sub-Subgenotype 2.1i Isolate of Classical Swine Fever Virus from China

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