We derive existence results of isotropic Jacobi fields on naturally reductive spaces and we prove that a naturally reductive space (M, g) of dimension ≤ 5 with the property that all Jacobi fields vanishing at two points are Tr(M, ∇)isotropic, for some adapted canonical connection ∇ and where Tr(M, ∇) denotes the corresponding transvection group, is locally symmetric. Moreover, for the three-dimensional case (M, g) is locally symmetric if all Jacobi fields vanishing at two points are isotropic.