Classification of microvascular patterns via cluster analysis reveals their prognostic significance in glioblastoma

Chen, Long; Zhang, Lin; Lin, Ge; Zhou, Chaowei; Yupeng, Chen; Wang, Xingfu; Zhou, Zheng

doi:10.1016/j.humpath.2014.10.002

Cited by 39 publications

(45 citation statements)

References 30 publications

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“…35 Again, different counting and labeling methods does not allow a direct comparison between our results and those previously obtained in the literature. On the other hand, we observed that MPNSTs with greater MVD in the periphery carry a worse prognosis, an issue previously demonstrated in astrocytomas, 46 glioblastomas, 47 prostate carcinomas, 48 and colorectal carcinomas. 49…”

Section: Discussionsupporting

confidence: 62%

Prognostic significance of mast cell and microvascular densities in malignant peripheral nerve sheath tumor with and without neurofibromatosis type 1

et al. 2019

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Malignant peripheral nerve sheath tumors (MPNSTs) are rare and aggressive soft tissue sarcomas with a significant susceptibility to metastasize early in their course. Pathogenesis is yet to be fully elucidated. Recently, the essential role of mast cells in the tumor onset of neurofibromatosis type 1 (NF1)‐associated neurofibromas and MPNSTs was confirmed in both experimental and human studies. In this study, we investigate mast cell density (MCD), microvascular density (MVD), and proliferation index (Ki‐67) in MPNST. A secondary aim was to correlate histological staining to clinical data and survival in patients with and without NF1. In total, 34 formalin‐fixed paraffin‐embedded MPNST tissues from 29 patients were eligible. MCD, MVD, and Ki‐67 labeling index (LI) were analyzed in all stained tissues by a computer‐based quantitative algorithm (Aperio ImageScope). In addition, chart review was performed for clinical data and survival analysis. Overall, MCD, MVD, and Ki‐67 LI were evenly distributed throughout tumor tissue. There was a negative correlation of NF1 status (affected, P = 0.037), tumor size (>10 cm, P = 0.023), and MVD in the tumor periphery (higher tercile, P = 0.002) to survival. Multivariate analysis confirmed the association of MVD in the tumor periphery (higher tercile, P = 0.019) with a decreased overall survival. Diverse mast cell and microvascular distributions suggest that angiogenesis in MPNST occurs independently. The role of mast cells in tumor progression is unclear and lacks prognostic value. Higher MVD has prognostic significance with possible therapeutic implications in MPNST.

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Section: Discussionsupporting

confidence: 62%

Prognostic significance of mast cell and microvascular densities in malignant peripheral nerve sheath tumor with and without neurofibromatosis type 1

et al. 2019

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“…Partitioning cluster analysis (CA) based on principal components (PCs) was applied to construct a hierarchical structure in all of the Class III malocclusion individuals. We performed CA by the k-means method, which sorted participants into groups by maximizing differences and minimizing differences [26, 27]. The clustering algorithm was performed separately for a range of 3 to 6 clusters.…”

Section: Methodsmentioning

confidence: 99%

Classification and characterization of class III malocclusion in Chinese individuals

Cai

Chen

et al. 2016

Head Face Med

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BackgroundClass III malocclusion is a maxillofacial disorder that is characterised by a concave profile and can be attributed to both genetic inheritance and environmental factors. It is a clinical challenge due to our limited understanding of its aetiology. Revealing its prototypical diversity will contribute to our sequential exploration of the underlying aetiological information. The objective of this study was to characterize phenotypic variations of Class III malocclusion via a lateral cephalometric analysis in a community of Chinese individuals.MethodOne-hundred-and-forty-four individuals (58 males ≥18 and 86 females ≥16) with Class III malocclusion ranging from mild to severe were enrolled in this study. Principal component analysis and cluster analysis were performed using 61 lateral cephalometric measurements.ResultsSix principal components were discovered in the examined population and were responsible for 73.7 % of the variability. Four subtypes were revealed by cluster analysis. Subtype 1 included subjects with mild mandibular prognathism with a steep mandibular plane. Subjects in subtype 2 showed a combination of prognathic mandibular and retrusive maxillary with a flat or normal mandibular plane. Subtype 3 included individuals with purely severe mandibular prognathism and a normal mandibular plane. Individuals in subtype 4 had a mild maxillary deficiency and severe mandibular prognathism with the lowest mandibular plane angle.ConclusionThe six principal components extracted among the 61 variables improve our knowledge of lateral cephalometric analysis for diagnoses. We successfully identified four Class III malocclusion subtypes, indicating that cluster analysis could supplement the classification of Class III malocclusion among a Chinese population and may assist in our on-going genetic study.Electronic supplementary materialThe online version of this article (doi:10.1186/s13005-016-0127-8) contains supplementary material, which is available to authorized users.

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“…A minority of the resident perivascular cells was closely associated with remodeled blood vessels forming capillary loops or glomeroloid bodies 35 , further suggesting reciprocity in the perivascular niche 8,10 . Previous studies demonstrated that glomeroloid bodies are populated by stem-like tumor cells in human tumor specimen 36 and this microvascular pattern is associated with a worse outcome in patients 37,38 .…”

Section: Discussionmentioning

confidence: 94%

Tumor cell plasticity, heterogeneity and resistance in crucial microenvironmental niches in glioma

Jung

Osswald

Ratliff

et al. 2020

Preprint

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Abstract Both the perivascular niche (PVN) and the integration into multicellular networks by tumor microtubes (TMs) have been associated with progression and resistance to therapies in glioblastoma, but their specific contribution remained unknown. By long-term tracking of tumor cell fate and dynamics in the live mouse brain, differential therapeutic responses in both niches could be determined. Both the PVN, a preferential location of long-term quiescent glioma cells, and network integration facilitated resistance against cytotoxic effects of radiotherapy and chemotherapy - independently of each other, but with additive effects. Perivascular glioblastoma cells were particularly able to actively repair damage to tumor regions. Population of the PVN and resistance in it depended on proficient NOTCH1 expression. In turn, NOTCH1 downregulation induced resistant multicellular networks by TM extension. Our findings identify NOTCH1 as a central switch between the PVN and network niche in glioma, and demonstrate robust cross-compensation when only one niche is targeted.

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Classification of microvascular patterns via cluster analysis reveals their prognostic significance in glioblastoma

Cited by 39 publications

References 30 publications

Prognostic significance of mast cell and microvascular densities in malignant peripheral nerve sheath tumor with and without neurofibromatosis type 1

Prognostic significance of mast cell and microvascular densities in malignant peripheral nerve sheath tumor with and without neurofibromatosis type 1

Classification and characterization of class III malocclusion in Chinese individuals

Tumor cell plasticity, heterogeneity and resistance in crucial microenvironmental niches in glioma

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