Classification of Mouse Sperm Motility Patterns Using an Automated Multiclass Support Vector Machines Model1

Goodson, Summer G.; Zhang, Zhaojun; Tsuruta, James K.; Wang, Wei; O’Brien, Deborah A.

doi:10.1095/biolreprod.110.088989

Cited by 142 publications

(165 citation statements)

References 43 publications

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“…Vigorous sperm motility is absolutely necessary for normal fertilization in mammals. 36 We measured the sperm motility in AMH-atg5 ¡/¡ and Atg5 Flox/Flox mice by computer-assisted semen analysis and found that the sperm motility of AMHatg5 ¡/¡ mice (15.00 § 1.83%) was severely decreased compared to that of Atg5 Flox/Flox mice (88.00 § 1.83%) (Fig. 2G) (Fig.…”

Section: The Disruption Of Autophagy In Sertoli Cells Perturbed the Smentioning

confidence: 94%

Autophagy is required for ectoplasmic specialization assembly in sertoli cells

et al. 2016

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The ectoplasmic specialization (ES) is essential for Sertoli-germ cell communication to support all phases of germ cell development and maturity. Its formation and remodeling requires rapid reorganization of the cytoskeleton. However, the molecular mechanism underlying the regulation of ES assembly is still largely unknown. Here, we show that Sertoli cell-specific disruption of autophagy influenced male mouse fertility due to the resulting disorganized seminiferous tubules and spermatozoa with malformed heads. In autophagy-deficient mouse testes, cytoskeleton structures were disordered and ES assembly was disrupted. The disorganization of the cytoskeleton structures might be caused by the accumulation of a negative cytoskeleton organization regulator, PDLIM1, and these defects could be partially rescued by Pdlim1 knockdown in autophagy-deficient Sertoli cells. Altogether, our works reveal that the degradation of PDLIM1 by autophagy in Sertoli cells is important for the proper assembly of the ES, and these findings define a novel role for autophagy in Sertoli cell-germ cell communication.

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Section: The Disruption Of Autophagy In Sertoli Cells Perturbed the Smentioning

confidence: 94%

Autophagy is required for ectoplasmic specialization assembly in sertoli cells

et al. 2016

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“…Motility parameters were analyzed in capacitated sperm from the different transgenic models using Hamilton-Thorne CASA system (Beverly, MA, USA) and CASAnova software as described (Goodson et al, 2011). Spontaneous and A23187 (10 µM)-induced acrosome reaction was measured as described (Wertheimer et al, 2008) in sperm from WT, Pyk2 −/− and Fer DR/DR mice.…”

Section: Motility and Acrosome Reaction Assaysmentioning

confidence: 99%

The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm

et al. 2016

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Sperm capacitation is required for fertilization. At the molecular level, this process is associated with fast activation of protein kinase A. Downstream of this event, capacitating conditions lead to an increase in tyrosine phosphorylation. The identity of the tyrosine kinase(s) mediating this process has not been conclusively demonstrated. Recent experiments using stallion and human sperm have suggested a role for PYK2 based on the use of small molecule inhibitors directed against this kinase. However, crucially, loss-of-function experiments have not been reported. Here, we used both pharmacological inhibitors and genetically modified mice models to investigate the identity of the tyrosine kinase(s) mediating the increase in tyrosine phosphorylation in mouse sperm. Similar to stallion and human, PF431396 blocks the capacitation-associated increase in tyrosine phosphorylation. Yet, sperm from Pyk2 −/− mice displayed a normal increase in tyrosine phosphorylation, implying that PYK2 is not responsible for this phosphorylation process. Here, we show that PF431396 can also inhibit FER, a tyrosine kinase known to be present in sperm. Sperm from mice targeted with a kinase-inactivating mutation in Fer failed to undergo capacitation-associated increases in tyrosine phosphorylation. Although these mice are fertile, their sperm displayed a reduced ability to fertilize metaphase II-arrested eggs in vitro.

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“…Sperm motility was then measured using the CEROS sperm analysis system (software version 12.3; Hamilton Thorne Biosciences, Beverly, MA). The analysis setting described previously (Goodson et al, 2011) was used. Sperm motility was also analyzed with an Olympus BX-53 microscope equipped with a highspeed camera (HAS-L1, Ditect, Tokyo, Japan).…”

Section: Sperm Motility Analysismentioning

confidence: 99%