Classifying Interactions in a Synthetic Bacterial Community Is Hindered by Inhibitory Growth Medium

Santos, Andrea R. Dos; Martino, Rita Di; Testa, Samuele; Mitri, Sara

doi:10.1128/msystems.00239-22

Cited by 5 publications

(2 citation statements)

References 62 publications

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“…To better characterise the interaction between P. aeruginosa and S. lugdunensis , we repeated the invasion assays using clinical isolates of P. aerugunisa from two patients where meropenem resistance evolved during infection and the de novo resistance mutations are known (Supplementary Table 1) ( 27, 28 ). A spent media assay was used for this invasion assay as the clinical isolates were not GFP-tagged ( 29 ). The invasion assay method above was followed with the following alterations.…”

Section: Methodsmentioning

confidence: 99%

Antibiotic resistance alters the ability ofPseudomonas aeruginosato invade the respiratory microbiome

Lindon,

Shah,

Gifford

et al. 2023

Preprint

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The emergence and spread of antibiotic resistance in bacterial pathogens is a global health threat. One important unanswered question is how antibiotic resistance influences the ability of a pathogen to invade the host-associated microbiome. Here we investigate how antibiotic resistance impacts the ability of the opportunistic bacterial pathogenPseudomonas aeruginosato invade the respiratory microbiome, by measuring the ability ofP. aeruginosaspontaneous antibiotic resistant mutants to invade pre-established cultures of commensal respiratory microbes. We find that commensal respiratory microbes tend to inhibit the growth ofP. aeruginosa, and antibiotic resistance is a double-edged sword that can either help or hinder the ability ofP. aeruginosato overcome this inhibition. The directionality of this help or hinderance depends on bothP. aeruginosagenotype and respiratory microbe identity. Antibiotic resistance facilitates the invasion ofP. aeruginosaintoStaphylococcus lugdunensis,yet impairs invasion intoRothia mucilaginosaandStaphylococcus epidermidis.Streptococcusspecies provide the strongest inhibition toP. aeruginosainvasion, and this is maintained regardless of antibiotic resistance genotype. Our study demonstrates how antibiotic resistance can alter the ability of a bacterial pathogen to invade the respiratory microbiome and suggests that attempts to manipulate the microbiome should focus on promoting the growth of commensals that can provide robust inhibition of both wildtype and antibiotic resistant pathogen strains.

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Section: Methodsmentioning

confidence: 99%

Antibiotic resistance alters the ability ofPseudomonas aeruginosato invade the respiratory microbiome

Lindon,

Shah,

Gifford

et al. 2023

Preprint

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“…The different populations within the community may have accidental interactions and their consequences can influence the dynamics of the consortia ( Gupta et al, 2020 ; Dos Santos et al, 2022 ). If the stabilization mechanism is absent among consortium members, therefore a fast-growing population can navigate the extinction of a slow-growing population ( Duncker et al, 2021 ).…”

Section: Strategies For the Selection Of Consortium Membersmentioning

confidence: 99%

Enhancing plant growth promoting rhizobacterial activities through consortium exposure: A review

Singh

Yadav

Chundawat

et al. 2023

Front. Bioeng. Biotechnol.

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Plant Growth Promoting Rhizobacteria (PGPR) has gained immense importance in the last decade due to its in-depth study and the role of the rhizosphere as an ecological unit in the biosphere. A putative PGPR is considered PGPR only when it may have a positive impact on the plant after inoculation. From the various pieces of literature, it has been found that these bacteria improve the growth of plants and their products through their plant growth-promoting activities. A microbial consortium has a positive effect on plant growth-promoting (PGP) activities evident by the literature. In the natural ecosystem, rhizobacteria interact synergistically and antagonistically with each other in the form of a consortium, but in a natural consortium, there are various oscillating environmental conditions that affect the potential mechanism of the consortium. For the sustainable development of our ecological environment, it is our utmost necessity to maintain the stability of the rhizobacterial consortium in fluctuating environmental conditions. In the last decade, various studies have been conducted to design synthetic rhizobacterial consortium that helps to integrate cross-feeding over microbial strains and reveal their social interactions. In this review, the authors have emphasized covering all the studies on designing synthetic rhizobacterial consortiums, their strategies, mechanism, and their application in the field of environmental ecology and biotechnology.

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Antibiotic resistance alters the ability of Pseudomonas aeruginosa to invade bacteria from the respiratory microbiome

Lindon,

Shah,

Gifford

et al. 2024

Evolution Letters

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The emergence and spread of antibiotic resistance in bacterial pathogens is a global health threat. One important unanswered question is how antibiotic resistance influences the ability of a pathogen to invade the host-associated microbiome. Here we investigate how antibiotic resistance impacts the ability of a bacterial pathogen to invade bacteria from the microbiome, using the opportunistic bacterial pathogen Pseudomonas aeruginosa and the respiratory microbiome as our model system. We measure the ability of P. aeruginosa spontaneous antibiotic-resistant mutants to invade pre-established cultures of commensal respiratory microbes in an assay that allows us to link specific resistance mutations with changes in invasion ability. While commensal respiratory microbes tend to provide some degree of resistance to P. aeruginosa invasion, antibiotic resistance is a double-edged sword that can either help or hinder the ability of P. aeruginosa to invade. The directionality of this help or hindrance depends on both P. aeruginosa genotype and respiratory microbe identity. Specific resistance mutations in genes involved in multidrug efflux pump regulation are shown to facilitate the invasion of P. aeruginosa into Staphylococcus lugdunensis, yet impair invasion into Rothia mucilaginosa and Staphylococcus epidermidis. Streptococcus species provide the strongest resistance to P. aeruginosa invasion, and this is maintained regardless of antibiotic resistance genotype. Our study demonstrates how the cost of mutations that provide enhanced antibiotic resistance in P. aeruginosa can crucially depend on community context. We suggest that attempts to manipulate the microbiome should focus on promoting the growth of commensals that can increase the fitness costs associated with antibiotic resistance and provide robust inhibition of both wildtype and antibiotic-resistant pathogen strains.

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Classifying Interactions in a Synthetic Bacterial Community Is Hindered by Inhibitory Growth Medium

Abstract: Communities of microbes colonize virtually every place on earth. Ultimately, we strive to predict and control how these communities behave, for example, if they reside in our guts and make us sick.

Cited by 5 publications

References 62 publications

Antibiotic resistance alters the ability ofPseudomonas aeruginosato invade the respiratory microbiome

Antibiotic resistance alters the ability ofPseudomonas aeruginosato invade the respiratory microbiome

Enhancing plant growth promoting rhizobacterial activities through consortium exposure: A review

Antibiotic resistance alters the ability of Pseudomonas aeruginosa to invade bacteria from the respiratory microbiome

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