Clavulanic acid: A review

Saudagar, Parag S.; Survase, Shrikant A.; Singhal, Rekha S.

doi:10.1016/j.biotechadv.2008.03.002

Cited by 108 publications

(111 citation statements)

References 94 publications

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“…Along these lines, to defeat this resistance, β-lactamase inhibitors are frequently utilized as a part of conjunction with β-lactam antibiotics as these mixes maintain the debasement of these antibiotics and increment the adequacy of these medications [6].…”

Section: Introductionmentioning

confidence: 99%

MOLECULAR AND MORPHOLOGICAL IDENTIFICATION OF STREPTOMYCES SP. NRC-88 NOVA SPECIES AS Β-Lactamase INHIBITOR FOR PHARMACEUTICAL APPLICATION

Awad

Germoush

2017

Asian J Pharm Clin Res

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Objective: Clavulanic acid (CA) is a vital agent in the treatment of bacterial infections since it is a potent inhibitor of an extensive variety of β-lactamase enzymes. Its production from Streptomyces strains is fact of expanding clinical significance. This study aimed to isolate and characterize a promising Streptomyces (S) species strain which produced an effective β-lactamase inhibitor.Methods: Streptomyces sp. NRC-88 was isolated from an Egyptian soil sample. The phenotypic and phylogenetic examinations of 16S rRNA gene were investigated. The active metabolite of this strain (CA) was determined by particular synergistic bioassay, spectrophotometric assay, recognized by thin layer chromatography, and structure of the CA affirmed by high-performance liquid chromatography (HPLC) method. Results:A phylogenetic examination of the 16S rRNA gene sequence of the NRC-88 strain consistent with conventional taxonomy was carried out, and confirmed that the strain NRC-88 was most similar to S. aburaviensis S-66 (99%). The active metabolite of this strain (CA) was determined by different methods and confirmed the structure of the CA by the HPLC method. It produced up to 87 mg/l in a specific CA production medium. Conclusion:A new species of Streptomyces sp. NRC-88 isolated and recognized by phenotypic and genotypic proof. This strain suggested the name, Streptomyces sp. NRC-88 (accession number KM014489). It was able to produce CA as the β-lactamase inhibitor.

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Section: Introductionmentioning

confidence: 99%

MOLECULAR AND MORPHOLOGICAL IDENTIFICATION OF STREPTOMYCES SP. NRC-88 NOVA SPECIES AS Β-Lactamase INHIBITOR FOR PHARMACEUTICAL APPLICATION

Awad

Germoush

2017

Asian J Pharm Clin Res

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“…O S. clavuligerus é uma bactéria Gram-Positiva, isolada a partir do solo, morfologicamente semelhante aos fungos filamentosos. Este micro-organismo é aeróbio estrito, cresce a temperatura ótima de 28 a 45ºC, e não utiliza a glicose como fonte de carbono (SAUDAGAR et al, 2008). Temperatura de fermentação, pH do meio de cultivo, agitação, aeração, concentração do produto, fonte de carbono e outros componentes do meio de cultivo são parâmetros que influenciam a produção do ácido clavulânico e de outros antibióticos (PEREIRA e OLIVEIRA, 2016).…”

Section: Introductionunclassified

Determinação Do Ph E Temperatura Ótimos Para a Produtividade Do Ácido Clavulânico Utilizando O Streptomyces Clavuligerus Atcc 27064

Pinelli¹,

Dias²

2017

MUNDI ETG

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Determinação do pH e temperatura ótimos para a produtividade do ácido clavulânico utilizando o Streptomyces clavuligerus ATCC 27064Determination of pH and temperature optimum on the productivity of clavulanic acid using Streptomyces clavuligerus ATCC 27064Juliana Junqueira Pinelli 1 Sandra de Cássia Dias 2 Resumo: O ácido clavulânico (AC) é um metabólito secundário produzido pela bactéria GramPositiva Streptomyces clavuligerus. É um composto de suma importância, principalmente para a indústria farmacêutica, pois possui ação antibacteriana. É utilizado em associação com outros antibióticos, como a amoxicilina. Sua produção ocorre por via fermentativa. O pH e a temperatura do meio são parâmetros relevantes que podem comprometer a produção deste metabólito. O presente trabalho teve como objetivo avaliar a influência da temperatura e do pH na produção de ácido clavulânico a partir de Streptomyces clavuligerus ATCC 27064. Para isso, um número mínimo de experimentos foi desenhado com diferentes valores de pH e temperatura utilizando o planejamento experimental estatístico, delineamento composto central rotacional, DCCR, tendo como variáveis pH e temperatura e como resposta a produtividade do ácido clavulânico. Amostras foram retiradas a cada 12 horas e a produção de ácido clavulânico determinada por método espectrofotométrico. O cultivo realizado em pH 6,7 e temperatura 27ºC apresentou maior produtividade do ácido clavulânico. Palavras-chave:Delineamento composto central rotacional. Antibiótico. Metodologia de superfície de resposta. Bioprocessos Abstract: Clavulanic acid (CA) is a secondary metabolite produced by the Gram-Positive bacteria Streptomyces clavuligerus. It is a compound of great importance, mainly for the pharmaceutical industry, because it has antibacterial activity. It is used in combination with other antibiotics such as amoxicillin. Its production occurs by fermentation. The pH and temperature of the medium are relevant parameters that can compromise the production of this metabolite. The aim of this study was to evaluate the temperature and pH influence on the clavulanic acid productivity by Streptomyces clavuligerus ATCC 27064. For this, a minimum number of experiments were designed with different values of pH and temperature using statistical experimental design, central composite rotational design (CCRD) having as variables pH and temperature and as response the clavulanic acid productivity. Samples were collected every 12 hours and the clavulanic acid production was determined by spectrophotometric method. The cultivation carried out at pH 6.7 and temperature 27ºC showed the highest clavulanic acid productivity.

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“…1) It is produced through the fermentation of S. clavuligerus, which requires a source of raw materials for the biosynthesis of cellular matter and products during normal cell operation, maintenance, and reproduction. 2) Raw materials originating in soybean have been used as the nitrogen source, and starch, dextrin, glycerol, and/or oils have been used as the carbon source. [3][4][5] However, although carbohydrates, including glucose, are the simplest energy source for cell growth and secondary metabolite production, rapid catabolism of them has been shown to decrease the rate of antibiotic biosynthesis.…”

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confidence: 99%

“…[3][4][5] However, although carbohydrates, including glucose, are the simplest energy source for cell growth and secondary metabolite production, rapid catabolism of them has been shown to decrease the rate of antibiotic biosynthesis. 2) At present, lipids and oils are considered not only to be essential medium components in the antibiotic industry, due to their natural antifoam properties, but also to be cheap alternative carbon sources. In addition, they have been found to increase secondary metabolite titers.…”

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confidence: 99%

“…11) In particular, residual oil levels have been reported to lead to increased medium viscosity and to affect further downstream processes. 2) Although there have been many studies suggesting that oil is a preferred carbon source for increasing CA titers, few studies have systematically examined the kinds of fatty acids that are important to CA production and the cell growth of the producer microorganisms. 9,12,13) To date, obtaining high titers of CA through strain improvements of S. clavuligerus has primarily depended on random mutagenesis and genetic engineering of the synthesis pathway of CA.…”

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confidence: 99%

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An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid inStreptomyces clavuligerus

Kim¹,

Kim²,

Shin³

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Clavulanic acid: A review

Cited by 108 publications

References 94 publications

MOLECULAR AND MORPHOLOGICAL IDENTIFICATION OF STREPTOMYCES SP. NRC-88 NOVA SPECIES AS Β-Lactamase INHIBITOR FOR PHARMACEUTICAL APPLICATION

MOLECULAR AND MORPHOLOGICAL IDENTIFICATION OF STREPTOMYCES SP. NRC-88 NOVA SPECIES AS Β-Lactamase INHIBITOR FOR PHARMACEUTICAL APPLICATION

Determinação Do Ph E Temperatura Ótimos Para a Produtividade Do Ácido Clavulânico Utilizando O Streptomyces Clavuligerus Atcc 27064

An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid inStreptomyces clavuligerus

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