Cleavable molecular beacon-based loop-mediated isothermal amplification assay for the detection of adulterated chicken in meat

Comparison of different DNA preparatory methods for development of a universal direct PCR-RFLP workflow for identification of meat origin in food products

“…The efficiency of species identification assays mostly relies on two main components; first, the extracted genomic DNA and second, the selected genomic region for identification. With time several methods have been developed either by following different PCR approaches (Song et al, 2018;Skouridou et al, 2019;Mokhtar et al, 2020;Batule et al, 2020;Yan et al, 2022) or by varying the genomic regions (Marchetti et al, 2020;Suryawan et al, 2020;Li et al, 2021;Tao et al, 2022) or both. Among such attempts, the direct PCR approach allows PCR amplification without prior DNA extraction.…”

Rapid analysis technologies with chemometrics for food authenticity field: A review

“…Recent research on LAMP-based meat identification has aimed to differentiate between the various animal origins of the meat. Meat products with species numbers as low as 2 (horse and donkey) ( Zhang et al, 2019 ) and as high as 10 (chicken, duck, pig, cow, horse, goat, rabbit, ostrich, camel and goose) ( Yan et al, 2022 ) were successfully distinguished, with detection limits as low as 10 fg. LAMP-based dairy research focuses on identifying specific sources of high-value milk, with samples tested on-site in as little as 30 min, effectively avoiding dairy product adulteration ( Kim and Kim, 2018 ).…”

Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing

“…Protein-based detection methods include liquid chromatography [7,8] and enzyme-linked immunosorbent assay [9,10] . Nucleic acid-based detection methods include multiplex PCR [11,12] , real-time fluorescent PCR [13,14] , droplet digital PCR [15] , restriction fragment length polymorphism [16] , and loop-mediated isothermal amplification [17] . The above methods require preset target species and are suitable for the identification of one or several species with known information.…”