Clickable poly(ester amine) dendrimer-grafted Fe3O4 nanoparticles prepared via successive Michael addition and alkyne–azide click chemistry

Abstract

Nanospheres, consisting of a Fe 3 O 4 core, a silica inner shell and dendritic poly(ester amine) (PEA) outer shell, were synthesized via the sol-gel reaction, and successive Michael addition and alkyne-azide click chemistry. The resultant PEA dendrimer-grafted magnetic nanoparticles (Fe 3 O 4 -g-PEA) were characterized by X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) spectroscopy, and thermogravimetric (TGA) analysis. Transmission electron microscopy (TEM) images reveal that the Fe 3 O 4 -g-PEA magnetic nanoparticles (MNPs) have a uniform size distribution of about 30 nm in diameter. The hydrodynamic size of Fe 3 O 4 -g-PEA MNPs changes reversibly with pH of the aqueous medium in the pH range of 3 to 9. The clickable alkyne groups on the surface of Fe 3 O 4 -g-PEA MNPs endow these nanoparticles with a functionalizable surface via alkyne-azide click chemistry. Assays with 3T3 fibroblasts and RAW macrophage cells indicate that the Fe 3 O 4 -g-PEA MNPs possess very low cytotoxicity. Hence, the PEA dendrimer-grafted magnetic nanospheres have potential applications as biomedical materials.

Magnetic properties of dendrimer structures with different coordination numbers: A Monte Carlo study

“…The temperature and field dependency of magnetization are similar to previous reports and are in line with the expectations for superparamagnetic nanoparticles [25][26][27].…”

Chimeric nanocomposites for the rapid and simple isolation of urinary extracellular vesicles

“…TEOS (98%) was added dropwise to the reaction mixture, which was then stirred overnight. Next, APTES (99%) was added dropwise to the mixture, which was then stirred overnight (Li et al., 2011 ). Fe 3 O 4 ‐NH 2 NPs were washed with methanol and dried in a vacuum desiccator for 1 h. The Fe 3 O 4 ‐NH 2 NPs, bis ‐ MPA, and p‐TSA were mixed and agitated at 200°C for 1 h (Fu et al., 2013 ).…”

“…The surfaces of proteins display many cationic groups, such as amine, imidazole, and guanidinium. Dendrimers and 2,2‐ bis (methylol)propionic acid ( bis ‐MPA) dendrimers (Carlmark et al., 2013 ) for the coating of NPs are modified onto the surfaces of the MNPs by using MNPs as core molecules for divergent growth of dendrimers with amine termini (Fu et al., 2013 ; Li et al., 2011 ). More functional hydroxyl groups on the surface can be converted to thiol functional groups and bind to the amine group of lactoferrin (LF) by cross‐linking (Dao et al., 2018 ).…”

Click chemistry: a new facile and efficient strategy for the preparation of Fe3O4 nanoparticles covalently functionalized with IDA-Cu and their application in the depletion of abundant protein in blood samples

“…Copper(I) catalyzed azide-alkyne cycloaddition (CuAAC) has emerged as a striking and promising synthesis approach owing to its unique ''click'' nature: namely, high yields, no byproducts, compatibility with water and moderate reaction conditions. 17 As a result, the CuAAC reaction has generated more and more attention in all areas of surface chemistry as an extremely chemoselective method for the immobilization of organic and biological molecules on diverse substrates, 18,19 such as the modification of bio-or synthetic polymers, 20,21 as well as the functionalization of gold, [22][23][24] mesoporous silica 25,26 and iron oxide [27][28][29] or FePt 30 NPs. Previously, in our group, we have exploited the preparation of polymer monolithic columns through click chemistry for the efficient separation of proteins.…”