Climate conditions and dietary practices during the Second Iron Age studied through the multi-isotope analysis of bones and teeth from individuals of Thézy-Glimont, Picardie, France

Clauzel, Thibault; Richardin, Pascale; Ricard, Jannick; Béchennec, Yves Le; Amiot, Romain; Fourel, François; Phouybanhdyt, Brian; Joseph, Diana; Vinçon‐Laugier, Arnauld; Flandrois, Jean‐Pierre; Lécuyer, Christophe

doi:10.1007/s12520-022-01534-1

Cited by 5 publications

(4 citation statements)

References 68 publications

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“…Humans are characterized by higher average δ 13 C coll (+3.2‰) and δ 15 N coll (+2.8‰) values than those recorded in animals (Figure 4a), as would be expected for an omnivorous diet based on C 3 -plants and animal products (meat and/or dairy), in agreement with studies performed on contemporaneous sites (e.g., Clauzel et al, 2022;Knipper et al, 2017). Sample #1649 stands out with high δ 13 C coll (À17.0 ± 0.1‰) and δ 15 N coll (+10.6 ± 0.1‰) values, indicating different dietary habits with the possible consumption of C 4 -plants or marine food.…”

Section: Diet Intra-population Isotopic Variations and The Social Sta...supporting

confidence: 89%

“…Oxygen stable isotope data have been published in Clauzel et al (2020) and consist of individuals from the nearby, contemporaneous site of Thézy-Glimont, which has been dated between 350 and 200 BCE. Based on archaeological, anthropological, and isotopic observations, the analyzed humans were interpreted as local and sedentary (Clauzel et al, 2020(Clauzel et al, , 2022. Therefore, the average δ 18 O p value for these individuals of +16.1 ± 0.6‰ (δ 18 O mw = À8.4 ± 1.0‰, n = 7) is used here as the local signature for the Picardie region during the La Tène B-C1 periods.…”

Section: Geographic Origin Of the Warriors Buried In Ribemont-sur-ancrementioning

confidence: 99%

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Geographic origin and social status of the Gallic warriors from Ribemont‐sur‐Ancre (France) studied through isotope systematics of bone remains

Clauzel

Richardin

Ricard

et al. 2022

Intl J of Osteoarchaeology

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Ribemont-sur-Ancre, France, is the biggest excavated human skeletal assemblage of Northern Gaul. It is interpreted as a post-battle deposit constructed to celebrate war.However, the chronology of the site, its organization and the origin of the buried warriors are still poorly understood. These problems are studied by a multi-proxy approach, based on radiocarbon dating, stable and radiogenic isotope analysis of bone remains from three structures within the site. A total of 47 human and 12 animal bone samples were analyzed for their oxygen isotope composition of bone bioapatite (δ 18 O p ), carbon and nitrogen isotope compositions of bone collagen (δ 13 C coll and δ 15 N coll ). Subsamples of 17 bone collagen and 20 bone samples were selected for radiocarbon dating and strontium isotope analysis ( 87 Sr/ 86 Sr), respectively. All samples show preservation from diagenesis, although 87 Sr/ 86 Sr contribution from the burial environment cannot be excluded. Therefore, the strontium isotope data are included in the study but are not used in the interpretation of the results. Homogeneous radiocarbon dates of human remains are observed, whereas later dates are obtained for some animal remains, highlighting the continuous use of the monument decades after the conflict. Human individuals have homogeneous δ 18 O p values, which are consistent with independent numismatic analyses and lead us to propose the Maine region as a potential geographic origin. Heterogeneities of δ 13 C coll and δ 15 N coll values are highlighted between individuals from different structures and are attributed to preferential access to animal protein and different social statuses among the buried warriors.

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Section: Diet Intra-population Isotopic Variations and The Social Sta...supporting

confidence: 89%

Section: Geographic Origin Of the Warriors Buried In Ribemont-sur-ancrementioning

confidence: 99%

Geographic origin and social status of the Gallic warriors from Ribemont‐sur‐Ancre (France) studied through isotope systematics of bone remains

Clauzel

Richardin

Ricard

et al. 2022

Intl J of Osteoarchaeology

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“…Sulfur isotopes in skeletal samples are used to study human diet and mobility in the Eastern Maya lowlands and Europe 265,266 using the "Longin method" of collagen extraction. 265,[267][268][269][270][271][272] Archeological bone samples are cleaned by air abrasion with Al 2 O 3 and then coarsely crushed ($1 mm) and dissolved in a 2.6 N HCl solution to eliminate mineral substances and some organic pollutants. Then the residue is heated with water (pH $ 3) at 90 C, shaken for hours, centrifuged or filtered with 5-8 μm filters, and the pure gelatine is collected.…”

Section: Skeletalmentioning

confidence: 99%

“…Sulfur isotopes in skeletal samples are used to study human diet and mobility in the Eastern Maya lowlands and Europe 265,266 using the “Longin method” of collagen extraction 265,267–272 . Archeological bone samples are cleaned by air abrasion with Al 2 O 3 and then coarsely crushed (~1 mm) and dissolved in a 2.6 N HCl solution to eliminate mineral substances and some organic pollutants.…”

Section: Chemical Treatmentmentioning

confidence: 99%

Procedures from samples to sulfur isotopic data: A review

Zhao,

Guo,

Zhang

et al. 2024

Rapid Comm Mass Spectrometry

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RationaleSulfur isotopes have been widely used to solve some key scientific questions, especially in the last two decades with advanced instruments and analytical schemes. Different sulfur speciation and multiple isotopes analyzed in laboratories worldwide and in situ microanalysis have also been reported in many articles. However, methods of sampling to measurements are multifarious, and occasionally some inaccuracies are present in published papers. Vague methods may mislead newcomers to the field, puzzle readers, or lead to incorrect data‐based correlations.MethodsWe have reviewed multiple methods on sulfur isotopic analyses from the perspectives of sampling, laboratory work, and instrumental analysis in order to help reduce operational inhomogeneity and ensure the fidelity of sulfur isotopic data. We do not deem our proposed solutions as the ultimate standard methods but as a lead‐in to the overall introduction and summary of the current methods used.ResultsIt has been shown that external contamination and transformation of different sulfur species should be avoided during the sampling, pretreatment, storage, and chemical treatment processes. Conversion rates and sulfur isotopic fractionations during sulfur extraction, purification, and conversion processes must be verified by researchers using standard or known samples. The unification of absence of isotopic fractionation is needed during all steps, and long‐term monitoring of standard samples is recommended.ConclusionThis review compiles more details on different methods in sampling, laboratory operation, and measurement of sulfur isotopes, which is beneficial for researchers' better practice in laboratories. Microanalyses and molecular studies are the frontier techniques that compare the bulk sample with the elemental analysis/continuous flow–gas source stable isotope ratio mass spectrometry method, but the latter is widely used. The development of sulfur isotopic measurements will lead to the innovation in scientific issues with sulfur proxies.

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Hydrogen isotope measurements of bone and dental tissues from archaeological human and animal samples and their use as climatic and diet proxies

Clauzel¹,

Richardin²,

Ricard³

et al. 2022

Journal of Archaeological Science

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Climate conditions and dietary practices during the Second Iron Age studied through the multi-isotope analysis of bones and teeth from individuals of Thézy-Glimont, Picardie, France

Cited by 5 publications

References 68 publications

Geographic origin and social status of the Gallic warriors from Ribemont‐sur‐Ancre (France) studied through isotope systematics of bone remains

Geographic origin and social status of the Gallic warriors from Ribemont‐sur‐Ancre (France) studied through isotope systematics of bone remains

Procedures from samples to sulfur isotopic data: A review

Hydrogen isotope measurements of bone and dental tissues from archaeological human and animal samples and their use as climatic and diet proxies

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