Climate-driven variation in the phenology of juvenile Ixodes pacificus on lizard hosts

Abstract

Background Ectothermic arthropods, like ticks, are sensitive indicators of environmental changes, and their seasonality plays a critical role in tick-borne disease dynamics in a warming world. Juvenile tick phenology, which influences pathogen transmission, may vary across climates, with longer tick seasons in cooler climates potentially amplifying transmission. However, assessing juvenile tick phenology is challenging in climates where desiccation pressures reduce the time ticks spend seeking blood meals. To improve our understanding of juvenile tick seasonality across a latitudinal gradient, we examine Ixodes pacificus phenology on lizards, the primary juvenile tick host in California, and explore how climate factors influence phenological patterns. Methods Between 2013 and 2022, ticks were removed from 1,527 lizards at 45 locations during peak tick season (March-June). Tick counts were categorized by life stage (larvae and nymphs) and linked with remotely sensed climate data. Juvenile phenology metrics, including abundance, date of peak abundance, and temporal overlap between larval and nymphal populations, were analyzed along a latitudinal gradient, including tick abundances on lizards, Julian date of peak mean abundance. Generalized Additive Models (GAMs) were applied to assess climate-associated variation in juvenile abundance on lizards. Results Mean tick abundance per lizard ranged from 0.17 to 47.21 across locations, with the highest in the San Francisco Bay Area and lowest in Los Angeles, where more lizards had zero ticks attached. In the San Francisco Bay Area, peak nymphal abundance occurred 25 days earlier than peak larval abundance. Temporal overlap between larval and nymphal stages at a given location varied regionally, with northern areas showing higher overlap. We found that locations with higher temperatures and increased drought stress were linked to lower tick abundances, though the magnitude of these effects depended on regional location. Conclusion Our study, which compiled 10 years of data, reveals significant regional variation in juvenile I. pacificus phenology across California, including differences in the abundance, peak timing, and temporal overlap. These findings highlight the influence of local climate on tick seasonality, with implications for tick-borne disease dynamics in a changing climate.