Clinical and experimental study of a terahertz time-domain system for the determination of the pathological margins of laryngeal carcinoma

Ke, Jing; Jia, Lixin; Hu, Yaqin; Xu, Jiang; Mo, Hailan; An, Xiang; Wang, Yuan

doi:10.1186/s12957-022-02788-8

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…Nevertheless, these studies have not yet enabled high resolution imaging of the fine structure of tissues. Moreover, current studies on the use of THz imaging to diagnose cancer are usually based on the difference of THz parameters between normal and cancerous tissue ( Chen et al, 2022 ; Ke et al, 2022 ; Wu et al, 2022 ). Our study provided a new idea for THz diagnosis of GC.…”

Section: Discussionmentioning

confidence: 99%

“…THz imaging system can be divided into different systems according to their structures. Based on terahertz pulsed imaging (TPI) system, researchers have been able to observe the boundary between cancerous and normal tissue in laryngeal cancer, breast cancer, and mouse brain glioma ( Oh et al, 2014 ; Chavez et al, 2018 ; Vohra et al, 2020 ; Ke et al, 2022 ). By using continuous-wave terahertz (CW THz) transmission or reflection scanning imaging system, the difference of THz images between cancerous and normal tissues in colon cancer and mouse brain glioma was detected respectively ( Wahaia et al, 2016 ; Wu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Early detection of gastric cancer via high-resolution terahertz imaging system

Shi

Zhao

et al. 2022

Front. Bioeng. Biotechnol.

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Terahertz (THz) wave has demonstrated a good prospect in recent years, but the resolution is still one of the problems that restrict the application of THz technology in medical imaging. Paraffin-embedded samples are mostly used in THz medical imaging studies, which are thicker and significantly different from the current gold standard slice pathological examination in sample preparation. In addition, THz absorption in different layers of normal and cancerous tissues also remains to be further explored. In this study, we constructed a high-resolution THz imaging system to scan non-tumorous adjacent tissue slices and gastric cancer (GC) tissue slices. In this system, a THz quantum cascade laser emitted a pulsed 3 THz signal and the transmitted THz wave was received by a THz detector implemented in a 65 nm CMOS process. The slice thickness was only 20 μm, which was close to that of the medical pathology examination. We successfully found THz transmittance differences between different layers of normal gastric tissues based on THz images, and the resolution could reach 60 μm for the first time. The results indicated that submucosa had a lower THz transmittance than that of mucosa and muscular layer in non-tumorous adjacent tissue. However, in GC tissue, THz transmittance of mucosa and submucosa was similar, caused by the decreased transmittance of mucosa, where the cancer occurs. Therefore, we suppose that the similar terahertz transmittance between gastric mucosa and submucosa may indicate the appearance of cancerization. The images obtained from our THz imaging system were clearer than those observed with naked eyes, and can be directly compared with microscopic images. This is the first application of THz imaging technology to identify non-tumorous adjacent tissue and GC tissue based on the difference in THz wave absorption between different layers in the tissue. Our present work not only demonstrated the potential of THz imaging to promote early diagnosis of GC, but also suggested a new direction for the identification of normal and cancerous tissues by analyzing differences in THz transmittance between different layers of tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Early detection of gastric cancer via high-resolution terahertz imaging system

Shi

Zhao

et al. 2022

Front. Bioeng. Biotechnol.

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“…A 96-well plate was applied to seed cells from each treatment group (at a denseness of 7.5 × 10 3 cells) and cultivated for 24 h, 48 h, and 72 h. Following this, the supernatant was dislodged, and 10 μl of CCK-8 solution was appended before being incubated for 2.5 h. Subsequently, using the SpectraMax 190 microplate reader (Molecular Devices, LLC., USA) to probe the absorbance at 450 nm.…”

Section: Methodsmentioning

confidence: 99%

“… 1 Laryngeal carcinoma (LC) is one of the most common cancers of HNC, accounting for approximately one-third of total HNC. 2 , 3 In recent years, the incidence of LC has continued to rise, with a 5-year overall survival rate of only 50%, and many patients are already in the advanced stage when discovered. 4 , 5 The treatment methods for LC include total laryngectomy, laser transoral throat microsurgery, radiotherapy, and chemotherapy, among which surgical resection is the most effective method.…”

Section: Introductionmentioning

confidence: 99%

NRSN2 promotes the malignant behavior of HPV-transfected laryngeal carcinoma cells through AMPK/ULK1 pathway mediated autophagy activation

Guo,

Wen,

et al. 2024

Cancer Biology & Therapy

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Neurensin-2 (NRSN2) performs a pro-carcinogenic function in multiple cancers. However, the function of NRSN2 in HPV-infected laryngeal carcinoma (LC) remains unclear. HPV transfection was performed in LC cells. The mRNA and protein levels were monitored using RT-qPCR, immunoblotting, and IF. Cell viability and proliferation were found using the CCK-8 assay and Edu staining. Cell invasion, migration, and apoptosis were probed using the Transwell, wound healing, and flow cytometry, respectively. The autophagosome was observed using TEM. NRSN2 was overexpressed in HPV-transfected LC cells. Inhibition of NRSN2 restrained the autophagy and malignant behavior of HPV-transfected LC cells. Meanwhile, the inhibition of AMPK/ULK1 pathway limited the increased autophagy of HPV-transfected LC cells caused by NRSN2 overexpression. Furthermore, NRSN2 knockdown inhibits autophagy by suppressing AMPK/ULK1 pathway, thereby restraining the malignant behavior of HPV-transfected LC cells. Our research confirmed that HPV transfection increased the autophagy and malignant behavior of LC cells by regulating the NRSN2-mediated activation of the AMPK/ULK1 pathway, offering a new target for cure of LC.

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“…However, as the frequency increases, the sample's absorption coefficient also increases leading to decreased THz wave penetration depth and Signal to Noise Ratio (SNR). Thus the frequency of 1.5THz has been found to clearly identify tumor area [202]. An optical near field techniques for example using air plasma based emitters or detectors has been proposed to overcome resolution issue in THz imaging [203].…”

Section: ) Diffraction Limited Spatial Resolution and Sensitivitymentioning

confidence: 99%

Terahertz Imaging and Sensing for Healthcare: Current Status and Future Perspectives

Gezimati

Singh

2023

IEEE Access

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There is a keen interest in the exploration of new generation emitters and detectors due to advancements in innovation of new materials and device processing technologies which have opened up new frontiers in the Terahertz (THz) spectrum. Therefore, it is necessary to review the developments in THz technology for healthcare applications, their impact, implications and prospects for ongoing research and development. This paper provides a broad overview of the current status and prospects of application of THz imaging and sensing for the healthcare domain. We present current knowledge, identify existing challenges for wide scale clinical adoption of THz systems and prospective opinions to facilitate research and development towards optimized and miniaturized THz systems and biosensors that provide real operational convenience through emerging trends. Firstly, we provide an overview of the THz imaging and sensing techniques that exploit properties of THz generation and detection with emphasis on terahertz time domain spectroscopy (THz-TDS) and THz Metamaterials. The mechanisms of tissue image contrast and the application of THz imaging and sensing for biomedical applications in particular, the cancer detection application is reported. Secondly, an outlook toward the advancements in THz technology in the interface of healthcare 4.0 and its enabling technologies is explored for next generation smart and connected healthcare systems. Third, we identify the merits and existing challenges in THz cancer imaging and sensing and suggest prospective opinions to pave way to ongoing and future research. Further, we discuss the recent advances in THz imaging development and the contribution of near-field techniques based on plasmonic, and resonance based metasurfaces, waveguides etc. for breaking the diffraction limit towards development of THz systems that are convenient for point of care. We bring researchers a roadmap for future research scope.

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Clinical and experimental study of a terahertz time-domain system for the determination of the pathological margins of laryngeal carcinoma

Cited by 10 publications

References 22 publications

Early detection of gastric cancer via high-resolution terahertz imaging system

Early detection of gastric cancer via high-resolution terahertz imaging system

NRSN2 promotes the malignant behavior of HPV-transfected laryngeal carcinoma cells through AMPK/ULK1 pathway mediated autophagy activation

Terahertz Imaging and Sensing for Healthcare: Current Status and Future Perspectives

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