Clinical and Laboratory Predictors for Forecasting the Outcomes of Ixodes Tick-Borne Borreliosis

Ns, Minoranskaya; Pv, Sarap; Nv, Andronova; Ei, Minoranskaya

doi:10.15690/vramn.v70i3.1337

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…Вместе с тем, при некоторых инфекционных заболеваниях, включая лихорадку денге и малярию, уровни концентраций цитокинов в сыворотке крови, наряду с биомаркерами окислительного стресса и лейкоцитарными индексами интоксикации, предлагается использовать в качестве прогностических критериев тяжести течения и риска развития осложнений заболевания [13][14][15][16]. Имеется лишь несколько публикаций, в которых авторы идентифицировали различные параметры иммунного и цитокинового статусов, позволяющие прогнозировать клинически неблагоприятное течение и исходы КЭ или ИКБ [3,17,18]. При этом поиск прогностических факторов, позволяющих различать эти две клещевые инфекции, может быть важен, поскольку в первую неделю заболевания до лабораторной верификации диагноза ранняя дифференциальная диагностика между лихорадочной формой (ЛФ) КЭ и безэритемной формой (БЭФ) ИКБ может быть затруднена из-за сходства клинической картины.…”

Section: Introductionunclassified

Study of the diagnostic and prognostic value of parameters of cytokine profile, oxidative stress and cytogenetic abnormalities in patients with tick-borne infections

Ilyinskikh,

Voronkova,

Samoylov

et al. 2024

jour

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Objective: is to assess parameters of cytokine profile, and some biomarkers of hemogram, cytogenetic damage, and oxidative stress to determine their associations with clinical symptoms and identify early prognostic factors for tick-borne encephalitis febrile form compared with Lyme borreliosis non-erythemal form at the onset of the disease.Materials and methods. In 25 patients with febrile tickborne encephalitis (mean age: 49.51 years), 24 patients with Lyme borreliosis non-erythemal form (mean age: 46.70 years) and 14 healthy donors (mean age: 47.20 years), the following parameters were analyzed: hemogram, blood leukocyte indices, cytokine profile, the levels of micronucleated T-lymphocytes, malondialdehyde and total immunoglobulin (Ig) G. For statistical analysis, the Mann–Whitney U-test, Pearson’s goodness-of-fit test, Spearman’s correlation coefficient, and ROC assay with AUC assessment were used.Results. In the tick-borne encephalitis patient group compared to the controls, the following parameters were increased: neutrophil count, blood leukocyte indices, the levels of micronucleated T-lymphocytes, malondialdehyde and proinflammatory cytokines in the blood, which directly correlated with maximal pyrexia levels. Prognostic value for early preliminary diagnosis of tick-borne encephalitis compared with borreliosis showed the following blood parameters: increases in neutrophil count and the levels of micronucleated T-cells, neutrophils to monocytes ratio, neutrophils to lymphocytes ratio, concentrations of malondialdehyde, tumor necrosis factor (TNF)-α, interleukins (IL)-8, IL-6, IL-10, and TNF-α/IL-4 ratio as well as decreases in the levels of IL-4 and total serum IgG. The parameters showed from average to excellent predictive ability as well as high positive and negative predictive values.Conclusion. In patients with tick-borne encephalitis, we detected correlations between the pyrexia levels and the biomarkers of inflammation, oxidative stress, and cytogenetic damage as well as it were found some differences in these parameters from the group of borreliosis patients, which probably had prognostic significance.

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Section: Introductionunclassified

Study of the diagnostic and prognostic value of parameters of cytokine profile, oxidative stress and cytogenetic abnormalities in patients with tick-borne infections

Ilyinskikh,

Voronkova,

Samoylov

et al. 2024

jour

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Analysis of leukogram parameters and blood cytokine profile in tick-borne infections of various etiologies

Воронкова

Ilyinskikh

Hasanova³

et al. 2023

Russian Journal of Infection and Immunity

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Introduction. Studies aimed at identifying pathogenetic features of tick-borne natural focal infections depending on etiological agent are relevant to seek out for new associations of biomarkers characterizing the structural and functional phenotype of immune cells significant for the differential diagnosis and prognosis of diseases. The aim of the study was to evaluate the parameters of a leukogram with an expanded profile in relation to the parameters of cytokine status in patients with viral tick-borne encephalitis as well as erythemic tick-borne borreliosis at acute stage of the disease. Materials and methods. The study involved 28 patients with the non-erythemic tick-borne borreliosis and 27 patients with tick-borne encephalitis at the acute stage of the disease, as well as 16 healthy individuals (control group). Venous blood samples were examined using a Sysmex XN1000 analyzer based on reflex testing technology with extended profile allowing to characterize leukocyte reaction during infection and inflammation, including parameters such as the absolute and relative count of immature granulocytes (IG), neutrophil granularity index (NEUT-GI), neutrophil reactivity index (NEUT-RI), count of reactive lymphocytes (RE-LYMP), count of antibody-producing lymphocytes (AS-LYMP). Concentration of IL-2, -4, -6, -8, -10, IFN and TNF in blood serum was determined by enzyme immunoassay using specific reagent kits (Vector-Best, Russia). Results. It was found that changes in the quantitative composition of blood leukocytes in patients with viral tick-borne encephalitis and non-erythemic form of tick-borne borreliosis have a unidirectional tendency characterized by increased count of metabolically active neutrophils (NEUT-RI) and a decreased count of reactive lymphocytes (RE-LYMP) compared to healthy individuals. Changes in the leukogram occur along with high blood concentration of proinflammatory cytokines IL-8 and TNF and a low level of IFN. A positive direct correlation was revealed between the fluorescence intensity parameter characterizing the metabolic activity of neutrophil granulocytes (NEUT-RI) and blood serum IL-8 level both in tick-borne encephalitis (r=0.422, p0.05) and non-erythemic form of tick-borne borreliosis (r=0.551, p0.05). Additionally, in the former, a positive correlation was established between the total leukocyte count (WBC) and concentration of TNF in the blood serum (r=0.532, p0.05).

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Predictive value of complete blood count for early diagnosis of mixed infection of the non-erythema migrans form of Lyme borreliosis and tick-borne encephalitis

Ilyinskikh

Filatova

Bulankov

et al. 2022

jour

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Objective: is to develop a model for early diagnosis of mixed infection of the non-erythema migrans form of Lyme borreliosis and tick-borne encephalitis using the assessment of the complete blood count and the blood leukocyte indices of patients in the first week of the disease.Materials and methods. The retrospective clinical study involved Group 1 of 27 patients with the mixed infection of the non-erythema migrans form of Lyme borreliosis and the febrile form of tick-borne encephalitis and Group 2 of 29 patients with the monoinfection of the non-erythema migrans form of Lyme borreliosis, who were hospitalized no later than in the 7th day of the disease. The average age of patients in Groups 1 and 2 was 50.6±3.4 and 49.9±2.3. We analyzed 14 parameters of the complete blood count as well as calculated the leukocyte intoxication index and the body resistance index. Statistical significance assessment was carried out using the chi-square test and ROC analysis. Logistic regression model was developed using STATISTICA 12.0 modules.Results. The levels of the band and polymorphonuclear neutrophils (<0,001 и p=0,002) and the leukocyte intoxication index (p<0,001) were significantly higher and the levels of the body resistance index (p<0.001), lymphocytes (p<0.001) and platelets (p=0.004) were lower in Group 1 than in Group 2. Informative predictors of mixed infection included the body resistance index (AUC=0.77), leukocyte intoxication index (AUC=0.75), the band and polymorphonuclear neutrophils (AUC=0.74), lymphocytes (AUC=0.77), and platelets (AUC=0.70). Logistic regression model has a “very good” predictive value (AUC=0.85) and include two parameters: body resistance index and platelets (×109/L).Conclusion. The developed model has a “very good” predictive value for early diagnosis of the mixed infection of the non-erythema migrans form of Lyme borreliosis and tickborne encephalitis before laboratory diagnosis confirmation.

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Clinical and Laboratory Predictors for Forecasting the Outcomes of Ixodes Tick-Borne Borreliosis

Cited by 4 publications

References 6 publications

Study of the diagnostic and prognostic value of parameters of cytokine profile, oxidative stress and cytogenetic abnormalities in patients with tick-borne infections

Study of the diagnostic and prognostic value of parameters of cytokine profile, oxidative stress and cytogenetic abnormalities in patients with tick-borne infections

Analysis of leukogram parameters and blood cytokine profile in tick-borne infections of various etiologies

Predictive value of complete blood count for early diagnosis of mixed infection of the non-erythema migrans form of Lyme borreliosis and tick-borne encephalitis

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