Clinical and Vitamin Response to a Short‐Term Multi‐Micronutrient Intervention in Brazilian Children and Teens: From Population Data to Interindividual Responses

Abstract

ScopeMicronutrients are in small amounts in foods, act in concert, and require variable amounts of time to see changes in health and risk for disease. These first principles are incorporated into an intervention study designed to develop new experimental strategies for setting target recommendations for food bioactives for populations and individuals.Methods and resultsA 6‐week multivitamin/mineral intervention is conducted in 9–13 year olds. Participants (136) are (i) their own control (n‐of‐1); (ii) monitored for compliance; (iii) measured for 36 circulating vitamin forms, 30 clinical, anthropometric, and food intake parameters at baseline, post intervention, and following a 6‐week washout; and (iv) had their ancestry accounted for as modifier of vitamin baseline or response. The same intervention is repeated the following year (135 participants). Most vitamins respond positively and many clinical parameters change in directions consistent with improved metabolic health to the intervention. Baseline levels of any metabolite predict its own response to the intervention. Elastic net penalized regression models are identified, and significantly predict response to intervention on the basis of multiple vitamin/clinical baseline measures.ConclusionsThe study design, computational methods, and results are a step toward developing recommendations for optimizing vitamin levels and health parameters for individuals.

Metabo groups in response to micronutrient intervention: Pilot study

“…Clinical biochemistry, micronutrient, dietary intake, genotype analyses, and plasma vitamin response were described previously (Mathias et al, 2018). After centrifugation, plasma was removed and 100 μl was frozen for iTRAQ proteomic analysis.…”

“…Genetic analysis and ancestry determination were previously analyzed and reported in Mathias et al (2018). Average ancestry data for individuals in each pool were calculated by summing percent of individual ancestry and dividing by 10.…”

“…This study was a subproject of the Harmonized Micronutrient Project (ClinTrials.gov # NCT01823744) that analyzed omics, biochemical, and nutritional status (Mathias et al, 2018) at baseline (time point or visit 1); after 6 weeks of daily supplementation of vitamins and minerals (time point or visit 2); and after 6 weeks of a washout period (time point or visit 3). Participants were healthy children and adolescents (ages 9-13) recruited from the west side of Ribeirão Preto (Brazil) in two county schools and one private school.…”

“…We (Mathias et al, 2018) and others (Kehdy et al, 2015;Rolim et al, 2016) have used admixture to better understand genetic and metabolite differences of individuals in subgroups of the Brazilian populations. We (Mathias et al, 2018) and others (Kehdy et al, 2015;Rolim et al, 2016) have used admixture to better understand genetic and metabolite differences of individuals in subgroups of the Brazilian populations.…”

“…We (Mathias et al, 2018) and others (Kehdy et al, 2015;Rolim et al, 2016) have used admixture to better understand genetic and metabolite differences of individuals in subgroups of the Brazilian populations. These results deserve further evaluation since vitamin levels may be implicated in reduction of LDL (Mathias et al, (2018)), an important predictor of cardiovascular disease. Linear regressions between ancestral components and baseline vitamin levels showed higher thiamine monophosphate (TMP) levels with higher European ancestry.…”