Clinical Application of Peripheral Nerve Transplantation

Mackinnon, Susan E.; Hudson, Alan R.

doi:10.1097/00006534-199210000-00024

Cited by 191 publications

(107 citation statements)

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“…4 In general, autologous nerve grafts provide optimal guidance cues for regenerating nerve fibers, such as peripheral glia cells and Schwann cells (SCs), which do not only produce regeneration-promoting factors but proliferate and also line their basal laminae up to the so-called bands of Büngner, the guiding tubes within the distal nerve segments. 5 Admittedly the use of autologous nerve tissue leads to loss of sensation at the site of harvest and the functional outcome after its utilization is described to range from extremely poor 6 to highly satisfying. 2 As an alternative to this, entubulation strategies, using biomaterials, have been performed.…”

mentioning

confidence: 99%

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

Wróbel

Serra

Ribeiro-Samy

et al. 2014

Tissue Engineering Part A

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Natural biomaterials have attracted an increasing interest in the field of tissue-engineered nerve grafts, representing a possible alternative to autologous nerve transplantation. With the prospect of developing a novel entubulation strategy for transected nerves with cell-seeded chitosan films, we examined the biocompatibility of such films in vitro. Different types of rat Schwann cells (SCs)-immortalized, neonatal, and adult-as well as rat bone-marrow-derived mesenchymal stromal cells (BMSCs) were analyzed with regard to their cell metabolic activity, proliferation profiles, and cell morphology after different time points of mono-and cocultures on the chitosan films. Overall the results demonstrate a good cytocompatibility of the chitosan substrate. Both cell types were viable on the biomaterial and showed different metabolic activities and proliferation behavior, indicating cell-type-specific cell-biomaterial interaction. Moreover, the cell types also displayed their typical morphology. In cocultures adult SCs used the BMSCs as a feeder layer and no negative interactions between both cell types were detected. Further, the chitosan films allow neurite outgrowth from dissociated sensory neurons, which is additionally supported on film preseeded with SC-BMSC cocultures. The presented chitosan films therefore demonstrate high potential for their use in tissue-engineered nerve grafts.

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mentioning

confidence: 99%

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

Wróbel

Serra

Ribeiro-Samy

et al. 2014

Tissue Engineering Part A

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“…Based on these facts, and following the concept of function-saving transplants, a number of isolated muscle, bone, joint, tendon, nerve and vessel allografts have been reported (25)(26)(27)(28)(29). More complex composite tissues, other than the hand, have also been successfully transplanted, such as the larynx, the face, the knee joint, the abdominal wall, and the uterus.…”

Section: Discussionmentioning

confidence: 99%

Hand Transplantation: The Milan Experience

Lanzettà¹,

Nolli²,

Vitale³

et al. 2007

Polish Journal of Surgery

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The authors describe the most critical aspects of transplantation of the hand. Three successful cases are presented from the Italian Hand Transplantation National Program. The key issues of this new type of surgical reconstruction for major amputations are selection criteria of suitable candidates, technical aspects of surgery, postoperative regime and functional rehabilitation. Other important aspects are staff requisites and organisation of the clinical trial, ethical and legal implications, dealing with the media. The authors comment on the fact that we might be ready to accept that almost anything could come from a cadaver, even if it is not a life-saving organ, to reconstruct missing parts and restore anatomical integrity and, especially, function.

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“…Advancements in our understanding of alloimmunity have resulted in a renewed interest in nerve allografts, which are now considered to be an available option for massive nerve tissue loss and a useful alternative to nerve autografts. The first description of the use of human peripheral nerve allografts provided encouraging results (Mackinnon, Hudson, 1992). However, peripheral nerve allograft transplantation requires tissue typing of both the donor and the recipient, and often transportation of graft materials over long distances.…”

Section: Introductionmentioning

confidence: 99%

Tetramethylpyrazine protects Schwann cells from ischemia-like injury and increases cell survival in cold ischemic rat nerves

Yang

Huang

Jiang

2015

Braz. J. Pharm. Sci.

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Tetramethylpyrazine (TMP), a major active ingredient of Ligusticum wallichi Franchat extract (a Chinese herb), exhibits neuroprotective properties in ischemia. In this study, we assessed its protective effects on Schwann cells (SCs) by culturing them in the presence of oxygen glucose deprivation (OGD) conditions and measuring cell survival in cold ischemic rat nerves. In the OGD-induced ischemic injury model of SCs, we demonstrated that TMP treatment not only reduced OGD-induced cell viability losses, cell death, and apoptosis of SCs in a dose-dependent manner, and inhibited LDH release, but also suppressed OGD-induced downregulation of Bcl-2 and upregulation of Bax and caspase-3, as well as inhibited the consequent activation of caspase-3. In the cold ischemic nerve model, we found that prolonged cold ischemic exposure for four weeks was markedly associated with the absence of SCs, a decrease in cell viability, and apoptosis in preserved nerve segments incubated in University of Wisconsin solution (UWS) alone. However, TMP attenuated nerve segment damage by preserving SCs and antagonizing the decrease in nerve fiber viability and increase in TUNEL-positive cells in a dose-dependent manner. Collectively, our results indicate that TMP not only provides protective effects in an ischemia-like injury model of cultured rat SCs by regulating Bcl-2, Bax, and caspase-3, but also increases cell survival and suppresses apoptosis in the cold ischemic nerve model after prolonged ischemic exposure for four weeks. Therefore, TMP may be a novel and effective therapeutic strategy for preventing peripheral nervous system ischemic diseases and improving peripheral nerve storage. Uniterms:Tetramethylpyrazine. Schwann cell. Oxygen glucose/deprivation. Peripheral nerve. Ischemia/ experimental study. University of Wisconsin solution.Tetrametilpirazina (TMP), o principal componente do extrato de Ligusticum wallichi Franchat (erva chinesa), apresenta propriedades neuroprotetoras na isquemia. Nesse estudo, avaliamos seus efeitos protetores nas células de Schwann (SC), cultivando-as na presença de condições de depleção de oxigênio da glicose (OGD) e medindo a sobrevivência dos nervos de ratos isquêmicos pelo resfriamento. No modelo de lesão isquêmica em SC induzida por OGD, demonstramos que o tratamento com TMP não somente reduziu as perdas de viabilidade celular induzida por OGD, a morte celular, a apoptose de SC dose-dependente e inibiu a liberação de LDH, mas, também, suprimiu a infra-regulação do Vcl-2 e a supra-regulação de Bax e caspase-3, e inibiu a consequente ativação da caspase-3. No modelo de nervo isquêmico por resfriamento, observamos que a exposição prolongada ao resfriamento por quatro semanas estava, marcadamente, associada com a ausência de SC, com o decréscimo da viabilidade celular e a apoptose em segmentos de nervo incubados na solução da Universidade de Wisconsin apenas. Entretanto, a TMP atenuou o dano no segmento do nervo preservando SC e antagonizando a diminuição da viabilidade da fibra nervosa e o aumento das cé...

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Clinical Application of Peripheral Nerve Transplantation

Cited by 191 publications

References 0 publications

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

Hand Transplantation: The Milan Experience

Tetramethylpyrazine protects Schwann cells from ischemia-like injury and increases cell survival in cold ischemic rat nerves

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