Clinical Comparison of the Baxter MicroScan Yeast Identification Panel™ and the Vitek Yeast Biochemical Card™

Riddle, Donna L.; Giger, Olarae; Miller, Linda A.; Hall, Gerri S.; Woods, Gail L.

doi:10.1093/ajcp/101.4.438

Cited by 17 publications

(10 citation statements)

References 15 publications

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“…The commercially available yeast identification methods (1,2,4,5,9,(13)(14)(15)17) have easily identified the common yeast species; however, uncommon and newly described species present a significant challenge. Phenotypic characteristics often cannot distinguish between species that have overlapping phenotypic characters.…”

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confidence: 99%

Experience with the MicroSeq D2 Large-Subunit Ribosomal DNA Sequencing Kit for Identification of Commonly Encountered, Clinically Important Yeast Species

2003

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Experience with a MicroSeq D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing kit for identification of yeast species commonly encountered in the mycology laboratory at Mayo Clinic is described here. A total of 131 isolates of yeasts recovered from clinical specimens were included in the study. Phenotypic methods used for initial identification included germ tube formation, urease production, microscopic morphological features on cornmeal agar, and an API 20C AUX system; all isolates were sequenced using a MicroSeq D2 LSU rDNA sequencing kit. Nucleic acid sequencing identified 93.9% of the isolates to the correct genus and species. A total of 100 of the isolates (representing 19 species of Candida) were sequenced, and 98% gave results concordant with identifications made by the API 20C AUX system; distance scores ranged from 0 to 1.88%, with an average value of 0.23%. Candida dubliniensis was not included in the MicroSeq database and was identified as Candida albicans. A total of 32 isolates representing 9 other genera (including Cryptococcus, Filobasidium, Kloeckera, Malassezia, Pichia, Sporidiobolus, Rhodotorula, Zygosaccharomyces, and Trichosporon) were included, and 81.3% showed concordant results when phenotypic and sequencing results were compared. Most discrepancies were attributed to the lack of inclusion of the species in the MicroSeq or API 20C AUX database. The MicroSeq D2 LSU rDNA sequencing kit appears to be accurate and useful for the identification of yeasts that might be seen in a clinical laboratory.

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confidence: 99%

Experience with the MicroSeq D2 Large-Subunit Ribosomal DNA Sequencing Kit for Identification of Commonly Encountered, Clinically Important Yeast Species

2003

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“…The API 20C system possesses promise for the differentiation of some dematiaceous fungi (3). By comparison, the latest updated Vitek yeast biochemical card provides correct identifications of between 89.7 and 97.2% (1,2,4,8). Isolates yielding incorrect or no identification with the Vitek system typically comprise strains that are uncommonly isolated from clinical specimens.…”

Section: Discussionmentioning

confidence: 99%

“…Isolates yielding incorrect or no identification with the Vitek system typically comprise strains that are uncommonly isolated from clinical specimens. Like the API 20C system, the majority of identifications with the Vitek system are available after 24 h of incubation, with 48 h of incubation being required for some slowly growing yeast iso- (6,8,12). The inability of the Mi-croScan system to accurately identify Cryptococcus neoformans is of concern.…”

Section: Discussionmentioning

confidence: 99%

Ability of RapID Yeast Plus System to identify 304 clinically significant yeasts within 5 hours

et al. 1996

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The RapID Yeast Plus System (Innovative Diagnostic Systems, Norcross, Ga.) is a qualitative micromethod that uses conventional and chromogenic substrates for the identification of medically important yeasts. The ability of the RapID Yeast Plus system to accurately identify 304 clinical yeast isolates within 5 h was evaluated. The RapID Yeast Plus method correctly identified 286 (94.1%) of strains to the species level without the need for additional tests. A further 12 strains (3.9%) were classified as correct to the genus level or to a lowprobability identification with two or more possibilities. In these latter cases, additional tests were required to delineate the correct identification. Organisms in the latter group comprised Candida parapsilosis (n ‫؍‬ 1), Candida tropicalis (n ‫؍‬ 1), Candida ciferrii (n ‫؍‬ 1), Candida guilliermondii (n ‫؍‬ 2), Candida humicola (n ‫؍‬ 1), Candida kefyr (n ‫؍‬ 1), Cryptococcus neoformans (n ‫؍‬ 1), and Rhodotorula rubra (n ‫؍‬ 4). Six strains (2.0%) were misidentified or did not yield codes in the manufacturer's database. These included one Candida utilis (identified as Candida famata/Candida guilliermondii), one Trichosporon beigelii (identified as Cryptococcus neoformans), one Candida diddensiae (identified as Candida albicans), one Candida membranaefaciens (identified as Candida parapsilosis), one Candida norvegensis (identified as Candida zeylanoides), and one Candida catenulata (no code) isolate; the last four strains are not included in the firm's current database. The RapID Yeast Plus system yielded excellent results and may be recommended for use in the routine laboratory for accurate same-day identification of clinically significant yeasts.

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“…Die Zahl der biochemischen Tests, die Anzahl der erfaBten Spezies und die Genauigkeit der Differenzierungsergebnisse ebenso wie die Kosten und der benotigte Zeitaufwand sind unterschiedlich. In mehreren Studien wurden die verschiedenen Testsysteme untereinander verglichen [4,5,6,71. Ein automatisiertes Verfahren zur SproRpilzidentifizierung ist der Vitek@-Automat (bioMerieux, Nurtingen), der seit 1976 weltweit in klinisch-mikrobiologischen Laboratorien im Einsatz ist [7].…”

Section: Introductionunclassified

Candida‐Identifizierung: Erfahrungen mit dem Vitek®‐Automaten

Schulz

Bernhardt

Schröder

1997

Mycoses

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We report about our experiences obtained with the Vitek system (bioMerieux, Nürtingen) for identification of 1160 clinical Candida isolates. The Vitek system correctly identified 1005 (86.6%) Candida isolates (probability > or = 85%): 776 (66.9%) strains were identified after 24 h of incubation, 229 (19.7%) required 48 h of incubation. In 4.4% (n = 51) we found results with a lower probability (< 85%). After 24 h of incubation 38 (14.6%) of the 260 C. tropicalis isolates tested were misidentified as C. parapsilosis; we obtained the correct results after 48 h of incubation. 104 (9%) isolates were wrongly identified after 48 h of incubation. The majority of these strains (n = 82) were biochemically minimally reactive Candida species. Overall, the Yeast Biochemical Card system provides a reliable method for the rapid, automated identification of medically important Candida species.

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Clinical Comparison of the Baxter MicroScan Yeast Identification Panel™ and the Vitek Yeast Biochemical Card™

Cited by 17 publications

References 15 publications

Experience with the MicroSeq D2 Large-Subunit Ribosomal DNA Sequencing Kit for Identification of Commonly Encountered, Clinically Important Yeast Species

Experience with the MicroSeq D2 Large-Subunit Ribosomal DNA Sequencing Kit for Identification of Commonly Encountered, Clinically Important Yeast Species

Ability of RapID Yeast Plus System to identify 304 clinically significant yeasts within 5 hours

Candida‐Identifizierung: Erfahrungen mit dem Vitek®‐Automaten

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