Clinical evaluation of the macroduct sweat collection system and conductivity analyzer in the diagnosis of cystic fibrosis

Hammond, Keith; Turcios, Nelson L.; Gibson, Lewis E.

doi:10.1016/s0022-3476(94)70314-0

Cited by 153 publications

(136 citation statements)

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“…O alto índice de massa corporal (IMC, kg/m 2 ) na infância está associado a 1) hiperlipidemia, resistência à insulina e hipertensão 2 ; e 2) obesidade e doença cardiovascular (DCV) na vida adulta 3,4 . Em muitos países em desenvolvimento, baixo peso ao nascer, baixo peso e nanismo ainda são prevalentes 5,6 , o que pode estar associado ao risco aumentado de DCV na vida adulta 7 . O duplo fardo representado por obesidade e baixo peso traz desafios econômicos e de saúde pública, especialmente nos países que estão atravessando uma transição socioeconômica.…”

Section: Is Obesity An Emerging Problem In Brazilian Children and Adounclassified

“…Dessa forma, o valor de concentração derivado é levemente mais alto do que o valor obtido por análise química direta dos íons de cloreto e sódio, já que outros eletrólitos, além do Cl-e do Na+, são inevitavelmente incluídos. Contudo, alguns estudos já foram realizados, demonstrando que há uma correlação íntima e uma razoável concordância (especialmente para os valores mais baixos) entre os valores de equivalentes de NaCl medidos por análise de condutividade e aqueles obtidos pela análise química quantitativa de Cl-e Na+ [5][6][7][8] .…”

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“…Dados confirmatórios têm sido fornecidos com relação a precisão, exatidão e reprodutibilidade: com relação à reprodutibilidade, testes repetidos no mesmo indivíduo fornecem resultados semelhantes 7 . Dados confirmatórios também têm sido fornecidos com relação à sua sensibilidade (capacidade de identificar um paciente com CF como tendo a doença) e sua especificidade (capacidade de identificar um indivíduo saudável como sendo saudável) [5][6][7][8][9] . Contudo, há alguma incerteza com relação aos critérios adotados nos 89 0021-7557/10/86-02/89…”

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Sweat testing: can the conductivity analysis take the place of the classic Gibson and Cooke technique?

Mastella¹

2010

J Pediatr (Rio J)

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Section: Is Obesity An Emerging Problem In Brazilian Children and Adounclassified

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Sweat testing: can the conductivity analysis take the place of the classic Gibson and Cooke technique?

Mastella¹

2010

J Pediatr (Rio J)

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“…The complete Macroduct collection and analysis system allows direct sweat analysis by conductivity measurements. However, although studies on conductivity reference values have been published by several groups, [10][11][12][13] so far consensus on reference values for sweat conductivity is missing. As outlined by Taylor et al, 14 deviation between conductivity and chloride concentration measurements increases at higher ionic strength, and no studies have been performed in patients with inconclusive or mild CF.…”

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Ion chromatography for the precise analysis of chloride and sodium in sweat for the diagnosis of cystic fibrosis

et al. 2014

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Background: Measurement of chloride in sweat is an essential part of the diagnostic algorithm for cystic fibrosis. The lack in sensitivity and reproducibility of current methods led us to develop an ion chromatography/high-performance liquid chromatography (IC/HPLC) method, suitable for the analysis of both chloride and sodium in small volumes of sweat. Methods: Precision, linearity and limit of detection of an in-house developed IC/HPLC method were established. Method comparison between the newly developed IC/HPLC method and the traditional Chlorocounter was performed, and trueness was determined using Passing Bablok method comparison with external quality assurance material (Royal College of Pathologists of Australasia). Results: Precision and linearity fulfill criteria as established by UK guidelines are comparable with inductively coupled plasma-mass spectrometry methods. Passing Bablok analysis demonstrated excellent correlation between IC/HPLC measurements and external quality assessment target values, for both chloride and sodium. With a limit of quantitation of 0.95 mmol/L, our method is suitable for the analysis of small amounts of sweat and can thus be used in combination with the Macroduct collection system. Conclusions: Although a chromatographic application results in a somewhat more expensive test compared to a Chlorocounter test, more accurate measurements are achieved. In addition, simultaneous measurements of sodium concentrations will result in better detection of false positives, less test repeating and thus faster and more accurate and effective diagnosis. The described IC/HPLC method, therefore, provides a precise, relatively cheap and easy-to-handle application for the analysis of both chloride and sodium in sweat.

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“…[14][15][16][17][18][19][20][21] One of these methods includes the use of the device Macroduct ® -a sweat collection system, 22 through which sweat is collected into a plastic coil after stimulation by pilocarpine iontophoresis. Weighting and risk of evaporation are thus eliminated.…”

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Comparação entre o método clássico de Gibson e Cooke e o teste da condutividade no suor em pacientes com e sem fibrose cística

Mattar

Gomes

Adde

et al. 2010

J. Pediatr. (Rio J.)

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Objective: To compare sweat chloride values obtained by quantitative pilocarpine iontophoresis (classic test) with the sweat conductivity values obtained using Macroduct ® collection system in patients with and without cystic fibrosis (CF). The cost and time spent to carry out each test were also analyzed. Methods:The sweat test using both techniques was performed at the same time in patients with and without CF. Conductivity cutoff values to rule out or diagnose CF were < 75 and ≥ 90 mmol/L, respectively, and for the classic test the chloride values were < 60 and ≥ 60 mmol/L. Results:Fifty-two patients with CF (29 males and 23 females; aged from 1.5 to 18.2 years) underwent the sweat test using both techniques, showing median sweat chloride and conductivity values of 114 and 122 mmol/L, respectively. In all of them, conductivity was ≥ 95 mmol/L, which provided the test with 100% sensitivity (95%CI 93.1-100). Fifty patients without CF (24 males and 26 females; aged from 0.5 to 12.5 years) had median sweat chloride and conductivity values of 15.5 and 30 mmol/L, respectively. In all cases, conductivity was < 70 mmol/L, which provided the test with 100% specificity (95%CI 92.9-100). Time spent to perform the tests was significantly shorter for the conductivity test, and its cost was also lower. Conclusions:The conductivity test showed high sensitivity and specificity, and there was good correspondence between the tests. The time spent to carry out the conductivity test was shorter and the cost was lower in comparison with the classic test.J Pediatr (Rio J). 2010;86(2):109-114: Cystic fibrosis, sweat test, conductivity, pilocarpine.

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Clinical evaluation of the macroduct sweat collection system and conductivity analyzer in the diagnosis of cystic fibrosis

Cited by 153 publications

References 6 publications

Sweat testing: can the conductivity analysis take the place of the classic Gibson and Cooke technique?

Sweat testing: can the conductivity analysis take the place of the classic Gibson and Cooke technique?

Ion chromatography for the precise analysis of chloride and sodium in sweat for the diagnosis of cystic fibrosis

Comparação entre o método clássico de Gibson e Cooke e o teste da condutividade no suor em pacientes com e sem fibrose cística

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