Clinical features of experimental trichinellosis in the raccoon dog (Nyctereutes procyonoides)

Näreaho, Anu; Sankari, Satu; Mikkonen, T.; Oivanen, Leena; Sukura, Antti

doi:10.1016/s0304-4017(00)00261-2

Cited by 15 publications

(16 citation statements)

References 14 publications

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“…Details on animal husbandry and clinical signs of the infection have been published elsewhere. 25 During the 12-week experimental period, blood was collected regularly: daily for the first week, then every second day for 3 weeks, and finally once weekly for 8 weeks. The Faculty of Veterinary Medicine (University of Helsinki) Committee on Animal Experiments approved the study.…”

Section: Methodsmentioning

confidence: 99%

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Trichinella nativaandT. spiralisInduce Distinguishable Histopathologic and Humoral Responses in the Raccoon Dog (Nyctereutes procyonoides)

et al. 2002

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Abstract. Three experimental groups of six male raccoon dogs (Nyctereutes procyonoides) each were formed by placing one of three littermates from six litters into each group. One group was inoculated with pigorigin Trichinella spiralis, the second was inoculated with raccoon dog-origin T. nativa, and the third served as a control group. The infective dose was 1,000 larvae/kg of body weight. Every third week, biopsies from M. triceps brachii were taken, and serum samples were collected for up to 12 weeks postinfection. In the early phase of the infection, cysts of both parasites were elongated cylinders that later became more spherical. However, at the end of the experiment, the cysts of T. nativa were more rounded than those of T. spiralis (mean length/width ϭ 2.5 versus 1.5 in T. spiralis versus T. nativa, respectively). Both species accumulated a collagen-rich capsule around the nurse cell, but the capsule was thicker in T. nativa. In both parasites, the total surface area of the sagittal section of the cyst was equal. Inflammation was more intense around T. nativa cysts. Specific antibodies were recognizable 2 weeks after infection by both enzyme-linked immunosorbent assay (ELISA) and western blot. In western blots, serum from both T. nativa-and T. spiralis-infected animals recognized the same components, but reaction with the homologous antigen was stronger. The same pattern was also seen in the ELISA. Immunoreactive epitopes were localized only in internal organs and cuticula of larvae in muscle.

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Section: Methodsmentioning

confidence: 99%

“…25 Here, we report differences in histopathologic and humoral responses of these two parasite species.…”

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confidence: 99%

Trichinella nativaandT. spiralisInduce Distinguishable Histopathologic and Humoral Responses in the Raccoon Dog (Nyctereutes procyonoides)

et al. 2002

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“…The filters were incubated from 1 h to overnight in blocking buffer (2% BSA, 25 mM Tris pH 8.0, 125 mM NaCl, 0.1% Tween 20, and 0.1% NaN 3 ) and then 1-3 h with primary antibody, experimentally Trichinella-infected raccoon dog sera (Nareaho et al 2000) diluted 1:100 in blocking buffer. Both homological and heterological antibodies were used.…”

Section: Western Blotmentioning

confidence: 99%

Comparative analysis of Trichinella spiralis and Trichinella nativa proteins by two-dimensional gel electrophoresis

et al. 2005

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Trichinella spiralis and Trichinella nativa are both common wildlife parasites in Finland. However, they differ substantially in their resistance to below 0 degrees C temperatures in their natural hosts. T. nativa can live in frozen fox meat for years, whereas T. spiralis dies when frozen. In mouse muscle, the difference is not as evident; even T. nativa cannot maintain infectivity when kept at -20 degrees C for 1 week. Crude larval protein extracts of these two parasite species were analyzed by two-dimensional gel electrophoresis (2DE). The protein patterns showed clear differences, but matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) peptide mass fingerprint followed by database searches failed to identify these proteins, suggesting that they may still be uncharacterized. The patterns compared after freezing treatment at -20 degrees C revealed changes in the intensity of some protein spots. The antigenic differences of the species were analyzed with two-dimensional Western blots, which showed T. spiralis-specific proteins.

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“…7 Raccoon dogs are frequent subjects of wildlife medicine literature, either as native, introduced, or research species. 2,8,11,12 However, this species is not common in zoos, and there is a lack of references of medical conditions that can affect this species in captivity. The present case report describes clinical and pathological characteristics of lymphoma with a leukemic phase in raccoon dogs.…”

Section: Introductionmentioning

confidence: 99%

“…The last routine physical examination performed 2.5 months earlier revealed no significant abnormalities other than mild symmetrical alopecia affecting the ventral neck and chest, caudolateral abdomen, and distal hind limbs; hematologic and biochemical analyses at that time were within normal limits for the species. 12 The following day, the raccoon dog was anesthetized using sevoflurane a delivered by facemask, and a complete physical examination was performed. Blood was collected from the cranial vena cava for hematologic and biochemical analyses.…”

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Clinical and pathologic characteristics of T-cell lymphoma with a leukemic phase in a raccoon dog (Nyctereutes procyonoides)

et al. 2011

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A 7.5-year-old raccoon dog ( Nyctereutes procyonoides) from the Henry Doorly Zoo (Omaha, Nebraska) presented to the veterinary hospital for lethargy and weight loss. On physical examination, splenomegaly and hepatomegaly were noted on palpation and were confirmed by radiographic evaluation. Radiography also demonstrated a mass in the cranial mediastinum. A complete blood cell count revealed marked leukocytosis (115,200 cells/µl), with a predominance of lymphoid cells. The animal was euthanized due to a poor prognosis. Necropsy revealed splenomegaly, hepatomegaly, and a large multiloculated mass in the cranial mediastinum. The histologic and immunohistochemical diagnosis was multicentric T-cell lymphoma with a leukemic phase.

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Clinical features of experimental trichinellosis in the raccoon dog (Nyctereutes procyonoides)

Cited by 15 publications

References 14 publications

Trichinella nativaandT. spiralisInduce Distinguishable Histopathologic and Humoral Responses in the Raccoon Dog (Nyctereutes procyonoides)

Trichinella nativaandT. spiralisInduce Distinguishable Histopathologic and Humoral Responses in the Raccoon Dog (Nyctereutes procyonoides)

Comparative analysis of Trichinella spiralis and Trichinella nativa proteins by two-dimensional gel electrophoresis

Clinical and pathologic characteristics of T-cell lymphoma with a leukemic phase in a raccoon dog (Nyctereutes procyonoides)

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