Clinical significance of the phosphorylation of MAPK and protein expression of cyclin D1 in human osteosarcoma tissues

Wu, Jian; Cui, Leilei; Yuan, Jun; Wang, Yuan; Song, Shan

doi:10.3892/mmr.2017.6224

Cited by 27 publications

(31 citation statements)

References 20 publications

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“…CCND1, located on chromosome 11q13, is a well-known oncogene frequently highly expressed in multiple human cancer types. CCND1 is also overexpressed in OS tissues [17]. OS patients with high CCND1 expression presented with shorter overall survival periods than those of patients with low CCND1 expression [18].…”

Section: Discussionmentioning

confidence: 97%

MicroRNA-34b attenuates proliferation and invasion of osteosarcoma cells by directly targeting CCND1

Hou¹,

Jiao²,

Sun³

et al. 2018

biomedicalresearch

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MicroRNAs (miRNAs) deregulation has been reported to play important roles in tumorigenesis and tumor development through the regulation of their targets in Osteosarcoma (OS). Hence, fully understanding the biological roles and mechanisms of OS-related miRNAs may contribute to the development of novel therapeutic targets for patients with this disease. In this study, we found that miR-34b expression was downregulated in OS tissues and cell lines. The resumption of miR-34b expression led to a significant reduction in cell proliferation and invasion of OS. Meanwhile, Cyclin D1 (CCND1) was identified as a direct target of miR-34b in OS through bioinformatics analysis, luciferase reporter assay, quantitative reverse-transcription PCR (RT-qPCR) and Western blot analysis. The results of the present study demonstrated that low miR-34b expression is involved in OS formation and progression, suggesting that miR-34b/CCND1 axis may serve as a potential therapeutic target in OS oncogenesis.

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Section: Discussionmentioning

confidence: 97%

MicroRNA-34b attenuates proliferation and invasion of osteosarcoma cells by directly targeting CCND1

Hou¹,

Jiao²,

Sun³

et al. 2018

biomedicalresearch

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“…Cyclin D1 is a key molecule for cells to transform from G1 phase to S phase (20). siRNA targeting Cyclin D1 can interfere with the expression of Cyclin D1 (21). This leads to tumor cell arrest in G1 phase.…”

Section: Discussionmentioning

confidence: 99%

“…This leads to tumor cell arrest in G1 phase. Inhibition of Cyclin D1 expression can change the distribution pattern of the cell cycle (21). The proportion of G0/1 phase increases, S phase decreases and G1/S increases significantly, indicating cell cycle arrest at G1/S.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression

Xue

Shen²,

Cui

et al. 2019

Exp Ther Med

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The present study aimed to investigate the function and mechanism of microRNA-638 (miR-638) in osteosarcoma. MiR-638 expression change in patients with osteosarcoma was detected by reverse transcription-quantitative polymerase chain reaction. Expression of miR-638 was observed to be downregulated in patients with osteosarcoma compared with the control group. In vitro , overexpression of miR-638 induced apoptosis, and inhibited cell proliferation and invasion of osteosarcoma cells. Overexpression of miR-638 induced Bcl-2 associated X and caspase-3 protein expression, and suppressed cyclin D1, phospholipase D1 (PLD1) and vascular endothelial growth factor (VEGF) protein expression in osteosarcoma. The promotion of PLD1 decreased the effects of miR-638 on osteosarcoma cell proliferation. In summary, it was demonstrated that miRNA-638 expression change in patients with osteosarcoma and an in vitro model via PLD1 and VEGF expression and miRNA-638 may be potential clinical indicators of osteosarcoma.

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“…CCND1 encoding Cyclin D1 is a member of cyclin family and plays a key role in regulating cell cycle progression ( 22 ). Increasing evidence indicated that CCND1 exerts an essential role in promoting the development and progression of human cancers, such as gastric cancer ( 23 ), glioma ( 24 ), renal cell cancer ( 25 ), lung adenocarcinoma ( 26 ) and ( 27 ). Except regulation of cell cycle, recent reports also indicated that CCND1 has a pivot role in promoting migration and tumor metastasis ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1

Cao

Fang²,

Teng³

et al. 2018

Exp Ther Med

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Emerging pieces of evidence indicate that microRNA-466 (miR-466) serves as a tumor suppressor in several human tumors, including colorectal cancer and prostate cancer. However, whether miR-466 is involved in osteosarcoma (OS) progression remains largely unknown. The present study demonstrated that miR-466 was significantly downregulated in OS tissues and cell lines. Furthermore, it was revealed that the expression of miR-466 was negatively correlated with OS severity. Moreover, low miR-466 expression in patients with OS predicted poor prognosis. Through functional experiments, miR-466 overexpression significantly inhibited the proliferation and cell cycle of OS cells while inducing cellular apoptosis. In terms of mechanism, it was revealed that CCND1 was a target of miR-466 in OS cells. miR-466 overexpression suppressed CCND1 expression in OS cells. A reverse association was observed between the expression levels of miR-466 and CCND1 in OS tissues. Furthermore, CCND1 restoration in OS cells significantly rescued the effects of miR-466 on cellular proliferation and apoptosis. Overall, the results of the present study demonstrated that miR-466 suppressed OS progression by targeting CCND1, suggesting that miR-466 may be a promising biomarker and therapeutic target for OS prognosis and treatment.

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Clinical significance of the phosphorylation of MAPK and protein expression of cyclin D1 in human osteosarcoma tissues

Cited by 27 publications

References 20 publications

MicroRNA-34b attenuates proliferation and invasion of osteosarcoma cells by directly targeting CCND1

MicroRNA-34b attenuates proliferation and invasion of osteosarcoma cells by directly targeting CCND1

MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression

MicroRNA‑466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1

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