Clinical study comparing probiotic Lactobacillus GR-1 and RC-14 with metronidazole vaginal gel to treat symptomatic bacterial vaginosis

Anukam, Kingsley C.; Osazuwa, Emmanuel O; Osemene, Gibson I; Ehigiagbe, Felix; Bruce, Andrew W.; Reid, Gregor

doi:10.1016/j.micinf.2006.08.008

Cited by 213 publications

(132 citation statements)

References 26 publications

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“…reuteri RC-14 is a vaginal isolate that has been studied extensively for its probiotic role in maintaining a healthy vaginal environment (30)(31)(32)(33). Our results demonstrate that L. reuteri RC-14 inhibited TSST-1 production, as well as the agr quorum-sensing system, in S. aureus MN8 with no impact on growth.…”

Section: Discussionmentioning

confidence: 71%

Lactobacillus reuteri -produced cyclic dipeptides quench agr -mediated expression of toxic shock syndrome toxin-1 in staphylococci

Wang²,

Xu³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

198

144

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The production of the staphylococcal exotoxin toxic shock syndrome toxin-1 (TSST-1) by Staphylococcus aureus has been associated with essentially all cases of menstruation-associated toxic shock syndrome (TSS). In this work, we show that the human vaginal isolate Lactobacillus reuteri RC-14 produces small signaling molecules that are able to interfere with the staphylococcal quorum-sensing system agr, a key regulator of virulence genes, and repress the expression of TSST-1 in S. aureus MN8, a prototype of menstrual TSS S. aureus strains. Quantitative real-time PCR data showed that transcription from the Ptst promoter, as well as the P2 and P3 promoters of the agr system from all four agr subgroups of S. aureus, was strongly inhibited in response to growth with L. reuteri RC-14 cultural supernatant. Alterations in the transcriptional levels of two other virulence-associated regulators sarA and saeRS were also observed, indicating a potential overall influence of L. reuteri RC-14 signals on the production of virulence factors in S. aureus. S. aureus promoter-lux reporter strains were used to screen biochemically fractionated L. reuteri RC-14 supernatant, and the cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Tyr-L-Pro) were identified as the signaling molecules. The results from this work contribute to a better understanding of interspecies cell-to-cell communication between Lactobacillus and Staphylococcus, and provide a unique mechanism by which endogenous or probiotic strains may attenuate virulence factor production by bacterial pathogens.diketopiperazine | two-component systems

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Section: Discussionmentioning

confidence: 71%

Lactobacillus reuteri -produced cyclic dipeptides quench agr -mediated expression of toxic shock syndrome toxin-1 in staphylococci

Wang²,

Xu³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

198

144

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“…This may have occurred due to strains themselves ascending to the vagina from the rectal area or altering the ability of the pathogens to transfer to this niche [14]. Thus, the probiotic supplementation along with standard antibiotic treatment was more beneficial as reported by Anukam et al [7].…”

Section: Resultsmentioning

confidence: 87%

“…The net result is an elevated pH, production of sialidase by colonizing foreign microbes [5] or amine production by them resulting in white discharge and fishy odor [6]. Vaginal infections can have symptoms, like itching, burning, and vaginal discharge [7]. If vaginosis is left untreated, it leads to some other complications like, pelvic inflammatory disease [8], infertility, pre-term birth, low birth weight etc.…”

Section: Introductionmentioning

confidence: 99%

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Clinical Study of Bacillus coagulans Unique IS-2 (ATCC PTA-11748) in the Treatment of Patients with Bacterial Vaginosis

2011

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Bacterial vaginosis (BV) is the most prevalent vaginal infection worldwide and is characterized by reduction of native lactobacilli. Antimicrobial therapy used to cure the disease is often found to be ineffective. We postulate that Bacillus coagulans Unique IS-2 (Unique Biotech Limited, India) might provide an appendage to antimicrobial treatment and improve curing rate. In the present study 40 Indian women diagnosed with BV by the presence of symptoms including white discharge, pH greater than 4.7, burning micturation, itching, soreness and redness at vulva. The subjects were divided in 2 groups probiotic (n = 20) and control (n = 20) based on age (control group, 33 ± 3 years and probiotic group, 32.5 ± 3 years), history of previous vaginosis (control group, 75% or 15/20 and probiotic group, 75% or 15/20) and severity of current vaginosis infection (burning micturation and itching, 35% in each group). Probiotic group subjects were assigned to receive a dose of antibiotic therapy [Ofloxacin-Ornidazole with strength of 200-500 mg per capsule/day for 5 days along with vaginal peccaries (co-kimaxazol) for 3 days] simultaneously with two probiotic capsules (10 9 CFUs of Bacillus coagulans Unique IS-2 per capsule). The control group received only antibiotic therapy. At the end of the treatment the 80% of probiotic group subjects showed significant positive response as revealed by reduction of vaginosis symptoms compared to the control group which exhibited reduction in 45% subjects only. Thus, the results of present study indicate that strain Bacillus coagulans Unique IS-2 can provide benefits to women being treated with antibiotics to cure an infectious condition.

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“…Так, D. Parent et al (1996) в 88 % случаев наблюдали нормализацию микрофлоры влага-лища в группе женщин, лечение БВ у которых проводилось путем интравагинального введе-ния капсул, содержащих L. acidophilus, в тече-ние 4 недель [65]. Позднее было проведено два клинических исследования с использованием пробиотиков, содержащих L. rhamnosus GR-1 и L. reuteri RC-14, в сравнении с применением метронидазола [66] [67], -с группой плацебо. Показано дву-кратное снижение заболеваемости БВ в группе с интравагинальным применением пробиоти-ков в сравнении с группой, в которой исполь-зовался метронидазол (65 и 33 % случаев соот-ветственно) [66], и выявлена эффективность лечения в 50 % случаев в группе с применением пробиотиков, содержащих L. brevis, L. salivarius и L. plantarum, в сравнении с группой плацебо (6 %) [67].…”

unclassified

Current views on vaginal microbiota and its impact on pregnancy outcomes

Siniakova

Александровна

2017

Z.akus.zen.bolezn

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ВведениеНевынашивание беременности -основ-ная причина перинатальной заболеваемости и смертности во всем мире и одна из важ-нейших проблем практического акушерства.Согласно данным ВОЗ частота встречаемости данной патологии в разных странах остается стабильно высокой и составляет 10-25 % от всех выявленных беременностей (20-25 % -самопроизвольные выкидыши, 5-10 % -пре- ■ Дисбиоз влагалища -одна из ведущих причин неблагоприятных исходов беременности. Основная роль в под-держании постоянства микробиоценоза влагалища принадлежит лактобациллам. Инфекционный процесс мо-гут вызвать как патогенные, так и условно-патогенные микроорганизмы, однако в подавляющем большинстве случаев возбудителями генитальной инфекции являются представители нормальной микрофлоры. Нарушение микробиоценоза влагалища в сочетании с несостоятельностью цервикального барьера ведет к миграции бак-терий в амниотическую полость и запуску патологических механизмов, приводящих к прерыванию беремен-ности. В статье представлены современные данные о роли инфекционного фактора в генезе невынашивания беременности. Рассмотрен вопрос о способности Lactobacillus iners предрасполагать к развитию патологической влагалищной микрофлоры. Уделено внимание основным подходам к профилактике и лечению дисбиотических нарушений. Затронут вопрос о применении бактериофагов в акушерстве и гинекологии.■ Ключевые слова: микробиоценоз влагалища; преждевременные роды; самопроизвольный выкидыш; бактери-альный вагиноз; аэробный вагинит. CURRENT VIEWS ON VAGINAL MICROBIOTA AND ITS IMPACT ON PREGNANCY OUTCOMES © A.A. SiniakovaThe ■ Vaginal dysbiosis is one of the leading causes of adverse pregnancy outcomes. The main role in maintaining the constancy of vaginal microbiocenosis belongs to lactobacilli. The infection process can be caused by both pathogenic and opportunistic microorganism, however, in the vast majority of cases vaginal infections is associated with the normal microflora. Violation of microbiocenosis of the vagina in combination with a cervical barrier failure leads to migration of bacteria in the amniotic cavity and the launch of the pathological mechanisms leading to pregnancy loss. The paper presents current data on the influence of vaginal infection on pregnancy loss. Consider the ability of Lactobacillus iners predispose to the development of pathological vaginal microflora. Attention was paid to the basic approaches to prevention and treatment of dysbiotic disorders. Discussion on the use of bacteriophages in obstetrics and gynecology.

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Clinical study comparing probiotic Lactobacillus GR-1 and RC-14 with metronidazole vaginal gel to treat symptomatic bacterial vaginosis

Cited by 213 publications

References 26 publications

Lactobacillus reuteri -produced cyclic dipeptides quench agr -mediated expression of toxic shock syndrome toxin-1 in staphylococci

Lactobacillus reuteri -produced cyclic dipeptides quench agr -mediated expression of toxic shock syndrome toxin-1 in staphylococci

Clinical Study of Bacillus coagulans Unique IS-2 (ATCC PTA-11748) in the Treatment of Patients with Bacterial Vaginosis

Current views on vaginal microbiota and its impact on pregnancy outcomes

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