Clinically Related Protein–Peptide Interactions Monitored in Real Time on Novel Peptide Chips by Surface Plasmon Resonance Imaging

Cherif, Boutheina; Roget, André; Villiers, Christian; Calemczuk, R.; Leroy, Vincent; Marche, Patrice N.; Livache, Thierry; Villiers, Marie‐Bernadette

doi:10.1373/clinchem.2005.058727

Cited by 64 publications

(55 citation statements)

References 33 publications

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“…Proteins (10 M) or peptides (100 M) were grafted in triplicate on the gold surface of the biochip by electrochemical copolymerization, as described in Grosjean et al (20) and Cherif et al (22), respectively. The binding of specific Ab to probes (proteins or peptides) was monitored using surface plasmon res- onance imaging (SPRi).…”

Section: Methodsmentioning

confidence: 99%

Protein-Peptide Arrays for Detection of Specific Anti-Hepatitis D Virus (HDV) Genotype 1, 6, and 8 Antibodies among HDV-Infected Patients by Surface Plasmon Resonance Imaging

et al. 2015

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iLiver diseases linked to hepatitis B-hepatitis D virus co-or superinfections are more severe than those during hepatitis B virus (HBV) monoinfection. The diagnosis of hepatitis D virus (HDV) infection therefore remains crucial in monitoring patients but is often overlooked. To integrate HDV markers into high-throughput viral hepatitis diagnostics, we studied the binding of anti-HDV antibodies (Abs) using surface plasmon resonance imaging (SPRi). We focused on the ubiquitous HDV genotype 1 (HDV1) and the more uncommon African-HDV6 and HDV8 genotypes to define an array with recombinant proteins or peptides. Fulllength and truncated small hepatitis D antigen (S-HDAg) recombinant proteins of HDV genotype 1 (HDV1) and 11 HDV peptides of HDV1, 6, and 8, representing various portions of the delta antigen were grafted onto biochips, allowing SPRi measurements to be made. Sixteen to 17 serum samples from patients infected with different HDV genotypes were injected onto protein and peptide chips. In all, Abs against HDV proteins and/or peptides were detected in 16 out of 17 infected patients (94.12%), although the amplitude of the SPR signal varied. The amino-terminal part of the protein was poorly immunogenic, while epitope 65-80, exposed on the viral ribonucleoprotein, may be immunodominant, as 9 patient samples led to a specific SPR signal on peptide 65 type 1 (65#1), independently of the infecting genotype. In this pilot study, we confirmed that HDV infection screening based on the reactivity of patient Abs against carefully chosen HDV peptides and/or proteins can be included in a syndromebased viral hepatitis diagnostic assay. The preliminary results indicated that SPRi studying direct physical HDAg-anti-HDV Ab interactions was more convenient using linear peptide epitopes than full-length S-HDAg proteins, due to the regeneration process, and may represent an innovative approach for a hepatitis syndrome-viral etiology-exploring array.

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Section: Methodsmentioning

confidence: 99%

Protein-Peptide Arrays for Detection of Specific Anti-Hepatitis D Virus (HDV) Genotype 1, 6, and 8 Antibodies among HDV-Infected Patients by Surface Plasmon Resonance Imaging

et al. 2015

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“…The polymerisation step was performed by a short 100 ms electrical pulse (2 V) between the counter electrode located in the needle of the microarrayer and the prism gold layer (Cherif et al, 2006, Villiers et al, 2009). Another grafting method based on electro-deposition of diazonium-peptide adducts (Corgier et al, 2009) was also used, as indicated in the text.…”

Section: Peptide Immobilization On Goldmentioning

confidence: 99%

“…Peptide microarrays are a good alternative, as peptides are shorter and their stability less dependant on their tridimensional structure. Thus they are often used for antibody profiling (Cherif et al, 2006, Halperin et al, 2011, Neuman de Vegvar et al, 2003. However, there is no study dealing with the stability of such microarrays during a large samples screening (>20).…”

Section: Introductionmentioning

confidence: 99%

Stability of Peptide in Microarrays: A Challenge for High-Throughput Screening

Villiers¹,

Brakha²,

Buhot³

et al. 2011

Electropolymerization

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“…In addition, studies using peptide arrays and chips have been constructed for the high-throughput analysis [17]. Surface plasmon resonance imaging has been a powerful measurement of the interactions in real time on peptide chips [18]. Moreover, a standardized discriminative model for the prediction of proteinpeptide interactions has been constructed using computer simulation [19].…”

Section: Introductionmentioning

confidence: 99%

Construction of a peptide with an electroactive daunomycin like a pendant arm to detect ovalbumin

Sugawara

Kadoya

Kuramitz

2015

Analytica Chimica Acta

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Clinically Related Protein–Peptide Interactions Monitored in Real Time on Novel Peptide Chips by Surface Plasmon Resonance Imaging

Abstract: Because of its characteristics (easy preparation of the peptide chip; high-throughput, label-free, real-time detection; high specificity; and low background), this technology is suitable for screening biological samples and for large-scale studies.

Cited by 64 publications

References 33 publications

Protein-Peptide Arrays for Detection of Specific Anti-Hepatitis D Virus (HDV) Genotype 1, 6, and 8 Antibodies among HDV-Infected Patients by Surface Plasmon Resonance Imaging

Protein-Peptide Arrays for Detection of Specific Anti-Hepatitis D Virus (HDV) Genotype 1, 6, and 8 Antibodies among HDV-Infected Patients by Surface Plasmon Resonance Imaging

Stability of Peptide in Microarrays: A Challenge for High-Throughput Screening

Construction of a peptide with an electroactive daunomycin like a pendant arm to detect ovalbumin

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