Clinically relevant analytical techniques, organizational concepts for application and future perspectives of point-of-care testing

Luppa, Peter B.; Bietenbeck, Andreas; Beaudoin, Christopher; Giannetti, Ambra

doi:10.1016/j.biotechadv.2016.01.003

Cited by 79 publications

(41 citation statements)

References 250 publications

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“…Further, portable biosensing platforms should exhibit biorecognition elements that remain stable for extended periods and at a variety of temperatures and humidity levels. The measurement robustness associated with the analysis of small sample volumes also requires further attention with the use of emerging low-cost materials, fabrication approaches, and transduction methods (Kumar et al 2013;Luppa et al 2016;Narayan, 2016;Wan et al 2013).…”

Section: Low-cost Single-use Portable Biosensorsmentioning

confidence: 99%

Electrochemical biosensors for pathogen detection

Cesewski

Johnson

2020

Biosensors and Bioelectronics

635

358

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A B S T R A C TRecent advances in electrochemical biosensors for pathogen detection are reviewed. Electrochemical biosensors for pathogen detection are broadly reviewed in terms of transduction elements, biorecognition elements, electrochemical techniques, and biosensor performance. Transduction elements are discussed in terms of electrode material and form factor. Biorecognition elements for pathogen detection, including antibodies, aptamers, and imprinted polymers, are discussed in terms of availability, production, and immobilization approach. Emerging areas of electrochemical biosensor design are reviewed, including electrode modification and transducer integration. Measurement formats for pathogen detection are classified in terms of sample preparation and secondary binding steps. Applications of electrochemical biosensors for the detection of pathogens in food and water safety, medical diagnostics, environmental monitoring, and bio-threat applications are highlighted. Future directions and challenges of electrochemical biosensors for pathogen detection are discussed, including wearable and conformal biosensors, detection of plant pathogens, multiplexed detection, reusable biosensors for process monitoring applications, and low-cost, disposable biosensors.

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Section: Low-cost Single-use Portable Biosensorsmentioning

confidence: 99%

Electrochemical biosensors for pathogen detection

Cesewski

Johnson

2020

Biosensors and Bioelectronics

635

358

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“…POCT is gaining increasing popularity due to its positive impact on improving health care decisions 36 . To date, POCT based on clinically useful antibodies has mainly involved the lateral flow immunoassay technology directed at the diagnosis of several infectious diseases (e.g.…”

Section: Discussionmentioning

confidence: 99%

Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes

Burbelo

Gunti

Keller

et al. 2017

Sci Rep

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Rapid point-of-care, antibody-based testing is not currently available for the diagnosis of most autoimmune and infectious diseases. Here we report a simple, robust and ultrafast fluid-phase immunocapture method for clinical measurements of antibody levels. This method employs neodymium magnetic sticks that capture protein A/G-coated paramagnetic beads bound to antibody-luciferase-labeled antigen complexes. We demonstrate the ability to effectively measure specific antibody levels in serum samples from patients with varied infectious or autoimmune disorders, and in the case of Sjögren’s syndrome directly in saliva, requiring about a minute per assay. We also show the feasibility of coupling this method with a hand-held luminometer for portable testing. Our method offers the potential to quickly diagnose a multitude of autoimmune and infectious diseases in point-of-care settings.

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“…Although the field of STI POCTs is advancing, initiatives to prompt the uptake of novel technologies have been scarce compared to the POCT field in other areas in clinical medicine 15. A literature review should be conducted to identify promising crossover initiatives, both for STIs and emerging infectious diseases.…”

Section: Basic Researchmentioning

confidence: 99%