Clinically relevant antibiotic-resistant bacteria in aquatic environments – An optimized culture-based approach

Schreiber, Christiane; Zacharias, Nicole; Essert, Sarah M.; Waßer, Felix; Müller, Heike; Sib, Esther; Precht, Tabea; Parčina, Marijo; Bierbaum, Gabriele; Schmithausen, Ricarda Maria; Kistemann, Thomas; Exner, Martin

doi:10.1016/j.scitotenv.2020.142265

Cited by 21 publications

(13 citation statements)

References 61 publications

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“…The water samples and the mussel cell suspensions were tested for the presence of antibiotic-resistant bacteria using chromogenic agar plates (CHROMagar ESBL, CHROMagar MRSA and CHROMagar VRE; MAST Diagnostica, Reinfeld, Germany), which are normally used for the isolation and differentiation of ARB from human material. A detailed description with performance characteristics for environmental samples was recently published by Schreiber et al (2021) [ 49 ]. Depending on the expected target bacteria and background flora, 1 mL and/or a dilution of the sample was spread directly on agar plates.…”

Section: Methodsmentioning

confidence: 99%

Antibiotic-Resistant Bacteria in Clams—A Study on Mussels in the River Rhine

et al. 2021

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Bacterial infections have been treated effectively by antibiotics since the discovery of penicillin in 1928. A worldwide increase in the use of antibiotics led to the emergence of antibiotic resistant strains in almost all bacterial pathogens, which complicates the treatment of infectious diseases. Antibiotic-resistant bacteria play an important role in increasing the risk associated with the usage of surface waters (e.g., irrigation, recreation) and the spread of the resistance genes. Many studies show that important pathogenic antibiotic-resistant bacteria can enter the environment by the discharge of sewage treatment plants and combined sewage overflow events. Mussels have successfully been used as bio-indicators of heavy metals, chemicals and parasites; they may also be efficient bio-indicators for viruses and bacteria. In this study an influence of the discharge of a sewage treatment plant could be shown in regard to the presence of E. coli in higher concentrations in the mussels downstream the treatment plant. Antibiotic-resistant bacteria, resistant against one or two classes of antibiotics and relevance for human health could be detected in the mussels at different sampling sites of the river Rhine. No multidrug-resistant bacteria could be isolated from the mussels, although they were found in samples of the surrounding water body.

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Section: Methodsmentioning

confidence: 99%

Antibiotic-Resistant Bacteria in Clams—A Study on Mussels in the River Rhine

et al. 2021

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“…Seven isolates were identified as Raoultella ornithinolytica (the closest accession was AJ251467). These seven isolates had a relatively small number (13)(14)(15)(16)(17) of variations in more than 1460 nucleotide sequences (more than 98.9-99.2% similarity) compared with those of K. aerogenes (Accession CP002824). The dominance of Escherichia/Shigella spp.…”

Section: Identification Of Species Of the Meropenem-resistant Isolatesmentioning

confidence: 99%

“…However, the number of reports on the quantitative detection of CRE in environmental samples is limited due to the presence of carbapenem-resistant bacteria other than Enterobacterales. Incubation at a higher temperature (42 °C) was effective for the selective growth of CRE in environmental samples which generally contain autochthonous species that have an intrinsic resistance to carbapenems, such as Stenotrophomonas maltophilia [ 17 , 18 ]. A high dominance of Aeromonas spp.…”

Section: Introductionmentioning

confidence: 99%

Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

2022

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The usefulness of wastewater-based epidemiology (WBE) was proven during the COVID-19 pandemic, and the role of environmental monitoring of emerging infectious diseases has been recognized. In this study, the prevalence of carbapenem-resistant Enterobacterales (CRE) in Japanese environmental samples was measured in the context of applying WBE to CRE. A total of 247 carbapenem-resistant isolates were obtained from wastewater, treated wastewater, and river water. Treated wastewater was shown to be an efficient target for monitoring CRE. The results of the isolate analysis showed that WBE may be applicable to Escherichia coli-carrying New Delhi metallo-β-lactamase (NDM)-type carbapenemase, the Enterobacter cloacae complex and Klebsiella pneumoniae complex-carrying IMP-type carbapenemase. In addition, a certain number of CRE isolated in this study carried Guiana extended spectrum (GES)-type carbapenemase although their clinical importance was unclear. Only a few isolates of Klebsiella aerogenes were obtained from environmental samples in spite of their frequent detection in clinical isolates. Neither the KPC-type, the oxacillinase (OXA)-type nor the VIM-type of carbapenemase was detected in the CRE, which reflected a low regional prevalence. These results indicated the expectation and the limitation of applying WBE to CRE.

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“…Today, the main negative impact of plastic presence in aquatic environmental is associated with the antibiotic-resistant bacterial strains [ 88 , 89 , 90 ]. Biofilms are difficult to remove as they have specific defense mechanisms against antimicrobial agents.…”

Section: Polymethacrylate: Applicationsmentioning

confidence: 99%

Acrylate and Methacrylate Polymers’ Applications: Second Life with Inexpensive and Sustainable Recycling Approaches

et al. 2021

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Polymers are widely employed in several fields thanks to their wide versatility and the easy derivatization routes. However, a wide range of commercial polymers suffer from limited use on a large scale due to their inert nature. Nowadays, acrylate and methacrylate polymers, which are respectively derivatives of acrylic or methacrylic acid, are among the most proposed materials for their useful characteristics like good biocompatibility, capping ability toward metal clusters, low price, potentially recyclability and reusability. Here, we discuss the advantages and challenges of this class of smart polymers focusing our attention on their current technological applications in medical, electronic, food packaging and environmental remediation fields. Furthermore, we deal with the main issue of their recyclability, considering that the current commercial bioplastics are not yet able to meet the global needs as much as to totally replace fossil-fuel-based products. Finally, the most accredited strategies to reach recyclable composites based on acrylic polymers are described.

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Clinically relevant antibiotic-resistant bacteria in aquatic environments – An optimized culture-based approach

Cited by 21 publications

References 61 publications

Antibiotic-Resistant Bacteria in Clams—A Study on Mussels in the River Rhine

Antibiotic-Resistant Bacteria in Clams—A Study on Mussels in the River Rhine

Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

Acrylate and Methacrylate Polymers’ Applications: Second Life with Inexpensive and Sustainable Recycling Approaches

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