Clitoria ternatea L. root extract ameliorated the cognitive and hippocampal long-term potentiation deficits induced by chronic cerebral hypoperfusion in the rat

Damodaran, Thenmoly; Tan, Byorn W.L.; Liao, Ping; Ramanathan, Surash; Lim, Gin Keat; Hassan, Zurina

doi:10.1016/j.jep.2018.06.020

Cited by 33 publications

(15 citation statements)

References 33 publications

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“…Intracellular calcium measurement has been described previously (Damodaran et al, 2018). In brief, primary cultured neurons were incubated in the dark for 30 min with 2.5 µM fura-2 acetoxymethyl (Fura-2 AM) (Thermo Fisher Scientific).…”

Section: Fura-2 Calcium Imagingmentioning

confidence: 99%

Mitragynine (Kratom) impairs spatial learning and hippocampal synaptic transmission in rats

et al. 2019

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Background: Mitragynine is the major alkaloid of Mitragyna speciosa (Korth.) or Kratom, a psychoactive plant widely abused in Southeast Asia. While addictive effects of the substance are emerging, adverse cognitive effects of this drug and neuropharmacological actions are insufficiently understood. Aims: In the present study, we investigated the effects of mitragynine on spatial learning and synaptic transmission in the CA1 region of the hippocampus. Methods: Male Sprague Dawley rats received daily (for 12 days) training sessions in the Morris water maze, with each session followed by treatment either with mitragynine (1, 5, or 10 mg/kg; intraperitoneally), morphine (5 mg/kg; intraperitoneally) or a vehicle. In the second experiment, we recorded field excitatory postsynaptic potentials in the hippocampal CA1 area in anesthetized rats and assessed the effects of mitragynine on baseline synaptic transmission, paired-pulse facilitation, and long-term potentiation. Gene expression of major memory-and addiction-related genes was investigated and the effects of mitragynine on Ca 2+ influx was also examined in cultured primary neurons from E16-E18 rats. Results/outcomes: Escape latency results indicate that animals treated with mitragynine displayed a slower rate of acquisition as compared to their control counterparts. Further, mitragynine treatment significantly reduced the amplitude of baseline (i.e. non-potentiated) field excitatory postsynaptic potentials and resulted in a minor suppression of long-term potentiation in CA1. Bdnf and αCaMKII mRNA expressions in the brain were not affected and Ca 2+ influx elicited by glutamate application was inhibited in neurons pre-treated with mitragynine. Conclusions/interpretation: These data suggest that high doses of mitragynine (5 and 10 mg/kg) cause memory deficits, possibly via inhibition of Ca 2+ influx and disruption of hippocampal synaptic transmission and long-term potentiation induction.

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Section: Fura-2 Calcium Imagingmentioning

confidence: 99%

Mitragynine (Kratom) impairs spatial learning and hippocampal synaptic transmission in rats

et al. 2019

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“…[ 30 ] The 2VO model has been widely reported to exhibit spatial learning and reference memory impairment due to the poor performance in Morris water maze task. [ 6–8,18 ] In this study, the 2VO rats had impaired learning and memory as in MWM they showed slower learning compared to the sham‐operated group. Hippocampus is the key structure for spatial learning and memory, and it is also one of the most susceptible regions to hypoperfusion, particularly in CA1 area.…”

Section: Discussionmentioning

confidence: 63%

“…Furthermore, the increasing synaptic strength was not maintained in 2VO rats after TBS and fell gradually throughout 2 h. Similar findings were found in other studies. [ 8,18,44,45 ] The result suggested that proteins involved in the synaptic plasticity might be attenuated in 2VO hippocampus rats. Thus, it can be speculated that neuronal damage might cause low expression of BDNF in the hippocampus of 2VO as seen in Western blot experiments and develop weak synaptic transmission as shown in LTP results.…”

Section: Discussionmentioning

confidence: 99%

“…After the incision was sutured, wound gel octenilin (Schulke & Mayr UK Ltd, Sheffield, UK) was applied on the incision area to prevent wound infection and the rats were kept individually in a transparent small cage to allow monitoring and recovery period for 7 days before the rats were pooled into a big cage that housed 5–7 rats per cage. [ 6,8,18 ] About 10% of the total rats subjected to 2VO developed drastic reductions in body weight and eye infections were excluded from the study.…”

Section: Methodsmentioning

confidence: 99%

“…[ 6,7 ] Moreover, this model has been widely used to investigate the effect of potential neuroprotective agents in CCH‐associated neurodegenerative disease models. [ 8–10 ] These studies demonstrated that the treatment of agents with antioxidant and anti‐inflammation properties or those that increase acetylcholine level in the brain can delay or prevent the progression of 2VO‐induced CCH‐associated learning and memory deficits.…”

Section: Introductionmentioning

confidence: 99%

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Xanthone-enriched fraction of Garcinia mangostana and α-mangostin improve the spatial learning and memory of chronic cerebral hypoperfusion rats

Tiang

Ahad

Murugaiyah

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Xanthones isolated from the pericarp of Garcinia mangostana has been reported to exhibit neuroprotective effect. Methods In this study, the effect of xanthone-enriched fraction of Garcinia mangostana (XEFGM) and a-mangostin (a-MG) were investigated on cognitive functions of the chronic cerebral hypoperfusion (CCH) rats. Key findings HPLC analysis revealed that XEFGM contained 55.84% of a-MG. Acute oral administration of XEFGM (25, 50 and 100 mg/kg) and a-MG (25 and 50 mg/kg) before locomotor activity and Morris water maze (MWM) tests showed no significant difference between the groups for locomotor activity. Conclusions However, a-MG (50 mg/kg) and XEFGM (100 mg/kg) reversed the cognitive impairment induced by CCH in MWM test. a-MG (50 mg/kg) was further tested upon sub-acute 14-day treatment in CCH rats. Cognitive improvement was shown in MWM test but not in long-term potentiation (LTP). BDNF but not CaMKII was found to be down-regulated in CCH rats; however, both parameters were not affected by a-MG. In conclusion, a-MG ameliorated learning and memory deficits in both acute and sub-acute treatments in CCH rats by improving the spatial learning but not hippocampal LTP. Hence, a-MG may be a promising lead compound for CCH-associated neurodegenerative diseases, including vascular dementia and Alzheimer's disease.

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Effects of Exercise on Long-Term Potentiation in Neuropsychiatric Disorders

Loprinzi

2020

Physical Exercise for Human Health

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Clitoria ternatea L. root extract ameliorated the cognitive and hippocampal long-term potentiation deficits induced by chronic cerebral hypoperfusion in the rat

Cited by 33 publications

References 33 publications

Mitragynine (Kratom) impairs spatial learning and hippocampal synaptic transmission in rats

Mitragynine (Kratom) impairs spatial learning and hippocampal synaptic transmission in rats

Xanthone-enriched fraction of Garcinia mangostana and α-mangostin improve the spatial learning and memory of chronic cerebral hypoperfusion rats

Effects of Exercise on Long-Term Potentiation in Neuropsychiatric Disorders

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