Clonagem e avaliação da expressão gênica do sbGnRH em machos juvenis e adultos de linguado, Paralichthys orbignyanus

Campos, Vinícius Farias; Collares, Tiago; Deschamps, João Carlos; Seixas, Fabiana Kömmling; Okamoto, Marcelo Hideo; Sampaio, Luís André; Marins, Luís Fernando; Robaldo, Ricardo Berteaux

doi:10.1590/s0102-09352011000100034

Cited by 5 publications

(1 citation statement)

References 28 publications

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“…Abreviações: Inoócito inicial; Pvtoócitos pré-vitelogênico; Vtoócito vitelogênico; POCcomplexo pós-ovulatório; GVBDoócitos maduros (oócitos com a quebra da vesícula germinativa); Atratresia; Sc1espermatócito primário; Sc2espermatócito secundário; Stespermatídes; Szespermatozoides. (AN et al, 2008;CAMPOS et al, 2011;KAVANAUGH;SOWER, 2008;KIM et al, 2011;SILVER et al. 2004).…”

Section: Razão Gonadossomáticaunclassified

Caracterização molecular dos GnRHs de Astyanax altiparanae (Garutti e Britski, 2000), seu efeito in vivo, e sua expressão temporal ao longo do estímulo reprodutivo.

Gomes¹

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Section: Razão Gonadossomáticaunclassified

Caracterização molecular dos GnRHs de Astyanax altiparanae (Garutti e Britski, 2000), seu efeito in vivo, e sua expressão temporal ao longo do estímulo reprodutivo.

Gomes¹

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Molecular characterization of different preproGnRHs in Astyanax altiparanae (Characiformes): Effects of GnRH on female reproduction

Chehade

Amaral

Branco

et al. 2020

Molecular Reproduction Devel

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Gonadotropin‐releasing hormone (GnRH) is a key molecule in the initiation of the hypothalamic–pituitary–gonadal axis. Thus, knowledge about GnRH may contribute to the effectiveness of species reproduction. Using a Neotropical tetra Astyanax altiparanae as a fish model species, the GnRH forms were characterized at the molecular level and the role of injected GnRHs in vivo was evaluated. The full‐length complementary DNA (cDNA) sequences of preproGnRH2 (612 bp) and preproGnRH3 (407 bp) of A. altiparanae were obtained, and the GnRH1 form was not detected. The cDNA sequences of preproGnRH2 and preproGnRH3 were found to be conserved, but a change in the amino acid at position 8 of the GnRH3 decapeptide of A. altiparanae was observed. All the injected GnRHs stimulated lhβ messenger RNA (mRNA) expression but not fshβ mRNA expression, and only GnRH2 was able to increase maturation‐inducing steroid (MIS) levels and possibly stimulate oocyte release. Furthermore, only GnRH2 was able to start the entire reproductive hormonal cascade and induce spawning.

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Neuropeptide Y gene expression around meal time in the Brazilian flounder Paralichthys orbignyanus

Campos¹,

Robaldo

Deschamps³

et al. 2012

J Biosci

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Neuropeptide Y (NPY) is considered the major stimulant for food intake in mammals and fish. Previous results indicate that NPY is involved in the feeding behaviour of the Brazilian flounder, Paralichthys orbignyanus. In this study, we evaluated hypothalamic NPY expression before (-2 h), during (0 h) and after feeding (+2 h) in two independent experiments: (1) during a normal feeding schedule and (2) in fish fasted for 2 weeks. During normal feeding, changes in the levels of NPY mRNA were periprandial, with expression levels being significantly elevated at meal time (P less than 0.05) and significantly reduced 2 h later (P less than 0.05). Comparing the fasting and unfasted groups, NPY mRNA levels were significantly higher (P less than 0.05) at -2 h and +2 h in the fasting group, but there was no difference at 0 h. In addition, the higher NPY mRNA levels that were observed in the fasting group were maintained throughout the sampling period. In summary, our results show that NPY expression was associated with meal time (0 h) in food intake regulation.

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Clonagem e avaliação da expressão gênica do sbGnRH em machos juvenis e adultos de linguado, Paralichthys orbignyanus

Cited by 5 publications

References 28 publications

Caracterização molecular dos GnRHs de Astyanax altiparanae (Garutti e Britski, 2000), seu efeito in vivo, e sua expressão temporal ao longo do estímulo reprodutivo.

Caracterização molecular dos GnRHs de Astyanax altiparanae (Garutti e Britski, 2000), seu efeito in vivo, e sua expressão temporal ao longo do estímulo reprodutivo.

Molecular characterization of different preproGnRHs in Astyanax altiparanae (Characiformes): Effects of GnRH on female reproduction

Neuropeptide Y gene expression around meal time in the Brazilian flounder Paralichthys orbignyanus

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