Clonal Analysis of Isolated Single Fundic and Pyloric Gland of Stomach Using X-Linked Polymorphism

Nomura, Sachiyo; Kaminishi, Michio; Sugiyama, Kenji; Oohara, Takeshi; Esumi, Hiroyasu

doi:10.1006/bbrc.1996.1365

Cited by 44 publications

(54 citation statements)

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“…PCR DNA was digested with RsaI and with or without HpaII, and the HUMARA was then amplified by PCR as previously described. 18) Amplification of the HUMARA in exon 1 was performed using primers AR1 and AR2, essentially as described by Mutter and Boynton 19) with slight modifications. Dimethylsulfoxide (DMSO; 6% w/v) and dGTP instead of 7-deaza-2′-dGTP were added.…”

Section: Methodsmentioning

confidence: 99%

Clonal Analysis of Adenosquamous Carcinoma of the Lung

Niho

Yokose

Kodama

et al. 1999

Japanese Journal of Cancer Research

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Adenosquamous carcinoma of the lung is a subset of pulmonary carcinomas, and comprises less than 4% of lung carcinomas. Its histogenesis remains unclear. The clonality of adenosquamous carcinoma from four female patients was analyzed to determine whether the clonality between the squamous cell and adenocarcinomatous components coincides. Each lesion was precisely microdissected from methanol-fixed sections. Adjacent normal lung tissue was collected as a normal control. DNA was extracted for clonal analysis based on an X-chromosome-linked polymorphic marker, the human androgen receptor gene (HUMARA). HUMARA was found to be amplified with or without previous digestion by the methylation-sensitive restriction endonuclease HpaII. All four cases were informative. Squamous cell and adenocarcinomatous components showed identical monoclonal patterns in all four patients. In one case, only the squamous cell carcinomatous component showed loss of heterozygosity of the HUMARA locus. The results suggest that the squamous cell and adenocarcinomatous components originate from the same cell.

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Section: Methodsmentioning

confidence: 99%

Clonal Analysis of Adenosquamous Carcinoma of the Lung

Niho

Yokose

Kodama

et al. 1999

Japanese Journal of Cancer Research

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“…Mathematical models predict that when murine antral glands are initially established, they contain approximately three stem cells 6 . These initially polyclonal glands resolve to a primarily monoclonal state over the first six weeks of life 4,6,7,9 , though a few residual polyclonal glands (5-10%) persist in the adult 9 .…”

Section: Introductionmentioning

confidence: 99%

Prospective Identification of a Multilineage Progenitor in Murine Stomach Epithelium

et al. 2007

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Background and Aims-Epithelial stem cells in the stomach are responsible for constant renewal of the epithelium through generation of multiple gastric cell lineages that populate the gastric glands. However, gastric stem or progenitor cells have not been well-characterized due to the lack of specific markers that permit their prospective recognition. We identified an intestinal promoter that is active in a rare subpopulation of gastric epithelial cells and investigated whether these cells possess multilineage potential.

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“…Studies of chimeric mammals (chimeric either as a result of random x-chromosome inactivation during development or as a result of mixing blastomeres from different mouse strains) show that most gastric units are monoclonal by adulthood, although they need not have started out that way (33,35,36,44,45). Thus, at some stage during development, there are single cells capable of generating all epithelial lineages.…”

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confidence: 99%

Multipotential stem cells in adult mouse gastric epithelium

Bjerknes

Cheng

2002

American Journal of Physiology-Gastrointestinal and Liver Physi

147

107

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Previous studies of chimeric animals demonstrate that multipotential stem cells play a role in the development of the gastric epithelium; however, despite much effort, it is not clear whether they persist into adulthood. Here, chemical mutagenesis was used to label random epithelial cells by loss of transgene function in adult hemizygous ROSA26 mice, a mouse strain expressing the transgene lacZ in all tissues. Many clones derived from such cells contained all the major epithelial cell types, thereby demonstrating existence of functional multipotential stem cells in adult mouse gastric epithelium. We also observed clones containing only a single mature cell type, indicating the presence of long-lived committed progenitors in the gastric epithelium. Similar results were obtained in duodenum and colon, showing that this mouse model is suitable for lineage tracing in all regions of the gastrointestinal tract and likely useful for cell lineage studies in other adult renewing tissues.

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Clonal Analysis of Isolated Single Fundic and Pyloric Gland of Stomach Using X-Linked Polymorphism

Cited by 44 publications

References 0 publications

Clonal Analysis of Adenosquamous Carcinoma of the Lung

Clonal Analysis of Adenosquamous Carcinoma of the Lung

Prospective Identification of a Multilineage Progenitor in Murine Stomach Epithelium

Multipotential stem cells in adult mouse gastric epithelium

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