Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

Abstract

Introduction: Salmonella is a major cause of foodborne disease, and poultry products are important contributors to the transmission of this zoonotic pathogen. Although considered to be rare in most countries, Salmonella Corvallis has been reported in specific geographic areas isolated from both human and non-human sources. The aim of this study was to report the occurrence, the antimicrobial resistance profiles including the extended-spectrum beta-lactamase (ESBL) production, and the clonal relatedness of S. Corvallis strains. Methodology: A total of 132 fragments of poultry carcasses from a slaughterhouse in São Paulo State, Brazil, were collected at different stages of the manufacturing process (post-bleeding, post-plucking, and post-chilling) and analyzed for the presence of Salmonella. Antimicrobial resistance was determined by disc diffusion method and Etest. Clonal relatedness was determined by pulsed-field gel electrophoresis (PFGE). Results: Among the 272 Salmonella strains recovered, fourteen were S. Corvallis. Ten (71.4%) showed ESBL production and resistance to at least three antimicrobial agents. Nalidixic acid resistance and reduced ciprofloxacin susceptibility was verified in four (28.6%) strains. PFGE analyses showed that all the S. Corvallis strains belonged to the same pulsotype. Conclusion: This study identified genetically related S. Corvallis strains exhibiting ESBL production and reduced susceptibility to quinolone. The results suggest the need to improve the sanitary conditions in the slaughterhouse. Moreover, from a public health perspective, continuous surveillance on Salmonella is needed to control the dissemination of this important zoonotic pathogen and its resistance determinants.

Prevalence of Salmonella Isolates and Their Distribution Based on Whole-Genome Sequence in a Chicken Slaughterhouse in Jiangsu, China

“…Moreover, the chickens slaughtered at this abattoir were from different farms. Seven S. Corvallis isolates were isolated in the first visit, in which the serotype was also reported in chicken from Brazil with an isolation rate of 7.9% (29). In the present study, the prevalence of S. Kentucky and S. Enteritidis in the slaughterhouse was 44.7 and 32.5%, which was consistent with findings reported in Guangdong province (27,28).…”

Risk factors associated with Salmonella prevalence, its antibiotic resistance, and egg antibiotic residues in the layer farming environment

“…Our study found two isolates of Typhimurium in the tap water outside the layer farmhouse, which may be due to Salmonella contamination from animal feces. S. enterica Corvallis is most commonly found in poultry carcasses following the slaughtering process in several countries, including Thailand [13], Brazil [14], Malaysia [15], and China [16]. In this study, the prevalence of Corvallis in laying farm environmental samples was high when compared with the results of Moraes et al [17] and Camba et al [18].…”

Highly Prevalent Multidrug-Resistant Salmonella From Chicken and Pork Meat at Retail Markets in Guangdong, China

“…Interestingly, Agona, Corvallis, and Kentucky were reported less frequently in previous studies ( Arnedo-Pena et al, 2016 ), whereas they were the dominant isolates in the current investigation. Additionally, serovar Agona is one of the top 10 serotypes responsible for nosocomial infections in China ( Zhang et al, 2014 ); serovar Corvallis has been reported in Brazil, Germany, and Turkey ( Kocabiyik et al, 2006 ; Fischer et al, 2013 ; Yamatogi et al, 2015 ); and serovar Kentucky was the most common serovar in the United States ( van Duijkeren et al, 2002 ; Galanis et al, 2006 ). The results suggest that Agona, Corvallis, and Kentucky may cause public health concerns in Guangdong.…”