Cloning and Characterization of a Gene Complementing the Mutation of an Ethanol-sensitive Mutant of<i>Sake</i>Yeast

Inoue, Toyohisa; Iefuji, Haruyuki; Fujii, Tsutomu; Soga, Hiroshi; Satoh, Kaname

doi:10.1271/bbb.64.229

Cited by 58 publications

(41 citation statements)

References 27 publications

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“…There have been at least 251 yeast genes identified that influence this character. However, candidacy of these genes for ethanol resistance was derived from testing their effect on the phenotypic variation by knocking them out (Inoue et al 2000;Takahashi et al 2001;Fujita et al 2006;van Voorst et al 2006) or from exploiting their functional alteration under ethanol stress treatment (Alexandre et al 2001). This indicates that yeast ET is under polygenic control as a typical quantitative trait.…”

Section: Discussionmentioning

confidence: 99%

“…So far, several studies have been carried out to identify the genes affecting ethanol tolerance in yeast by testing performance of the reference yeast strain that was genetically modified at different candidate genes under an ethanol stress condition (Inoue et al 2000;Kajiwara et al 2000;Takahashi et al 2001;Fujita et al 2006;van Voorst et al 2006). The candidacy of the genes was determined mainly according to the genes' involvement in the previously mentioned biochemical or physiological pathways.…”

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confidence: 99%

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Genetic Dissection of Ethanol Tolerance in the Budding Yeast Saccharomyces cerevisiae

et al. 2007

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Uncovering genetic control of variation in ethanol tolerance in natural populations of yeast Saccharomyces cerevisiae is essential for understanding the evolution of fermentation, the dominant lifestyle of the species, and for improving efficiency of selection for strains with high ethanol tolerance, a character of great economic value for the brewing and biofuel industries. To date, as many as 251 genes have been predicted to be involved in influencing this character. Candidacy of these genes was determined from a tested phenotypic effect following gene knockout, from an induced change in gene function under an ethanol stress condition, or by mutagenesis. This article represents the first genomics approach for dissecting genetic variation in ethanol tolerance between two yeast strains with a highly divergent trait phenotype. We developed a simple but reliable experimental protocol for scoring the phenotype and a set of STR/SNP markers evenly covering the whole genome. We created a mapping population comprising 319 segregants from crossing the parental strains. On the basis of the data sets, we find that the tolerance trait has a high heritability and that additive genetic variance dominates genetic variation of the trait. Segregation at five QTL detected has explained $50% of phenotypic variation; in particular, the major QTL mapped on yeast chromosome 9 has accounted for a quarter of the phenotypic variation. We integrated the QTL analysis with the predicted candidacy of ethanol resistance genes and found that only a few of these candidates fall in the QTL regions.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Genetic Dissection of Ethanol Tolerance in the Budding Yeast Saccharomyces cerevisiae

et al. 2007

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“…3,4) A number of factors that affect ethanol tolerance of yeast have been investigated, such as the trehalose content of yeast cells, 5) the degree of unsaturation of fatty acids in the cellular membrane, and the ergosterol content of yeast cells. 9) An ethanolsensitive mutant isolated by us from sake yeast was found to have a mutation in the erg6 gene, which is involved in the biosynthesis of ergosterol. Since the ERG6 gene complements the ethanol sensitivity of this mutant, it is clear that the ergosterol contained in yeast cells plays an important role in ethanol tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…Since the ERG6 gene complements the ethanol sensitivity of this mutant, it is clear that the ergosterol contained in yeast cells plays an important role in ethanol tolerance. 9) As for genes related to ethanol tolerance, as described above, the genes involved in trehalose biosynthesis, TPS1, TPS2, and TSL1, were overexpressed in K-9 under shaking conditions. OLE1, involved in the biosynthesis of unsaturation of fatty acids, 10) was overexpressed under both conditions.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Expression Profile of Sake Brewing Yeast under Shaking and Static Conditions

Shobayashi

Ukena

Fujii

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…HAP1 encodes a heme-mediated transcription factor that controls the expression of genes involved in fermentation (Mense and Zhang 2006) and in the biosynthesis of ergosterol, which is important for ethanol tolerance (Inoue et al 2000). As in other industrial strains (Tamura et al 2004), this interrupting Ty1 element is absent in JAY291.…”

Section: Overview Of Specific Gene Polymorphisms Important For Bioethmentioning

confidence: 99%

Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production

Argueso

Carazzolle

Mieczkowski³

et al. 2009

Genome Res.

253

236

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Bioethanol is a biofuel produced mainly from the fermentation of carbohydrates derived from agricultural feedstocks by the yeast Saccharomyces cerevisiae. One of the most widely adopted strains is PE-2, a heterothallic diploid naturally adapted to the sugar cane fermentation process used in Brazil. Here we report the molecular genetic analysis of a PE-2 derived diploid (JAY270), and the complete genome sequence of a haploid derivative (JAY291). The JAY270 genome is highly heterozygous (;2 SNPs/kb) and has several structural polymorphisms between homologous chromosomes. These chromosomal rearrangements are confined to the peripheral regions of the chromosomes, with breakpoints within repetitive DNA sequences. Despite its complex karyotype, this diploid, when sporulated, had a high frequency of viable spores. Hybrid diploids formed by outcrossing with the laboratory strain S288c also displayed good spore viability. Thus, the rearrangements that exist near the ends of chromosomes do not impair meiosis, as they do not span regions that contain essential genes. This observation is consistent with a model in which the peripheral regions of chromosomes represent plastic domains of the genome that are free to recombine ectopically and experiment with alternative structures. We also explored features of the JAY270 and JAY291 genomes that help explain their high adaptation to industrial environments, exhibiting desirable phenotypes such as high ethanol and cell mass production and high temperature and oxidative stress tolerance. The genomic manipulation of such strains could enable the creation of a new generation of industrial organisms, ideally suited for use as delivery vehicles for future bioenergy technologies.

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Cloning and Characterization of a Gene Complementing the Mutation of an Ethanol-sensitive Mutant ofSakeYeast

Cited by 58 publications

References 27 publications

Genetic Dissection of Ethanol Tolerance in the Budding Yeast Saccharomyces cerevisiae

Genetic Dissection of Ethanol Tolerance in the Budding Yeast Saccharomyces cerevisiae

Genome-Wide Expression Profile of Sake Brewing Yeast under Shaking and Static Conditions

Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production

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