Cloning and characterization of peanut allene oxide cyclase gene involved in salt-stressed responses

Liu, H H; Wang, Y G; Wang, S P; Li, H J

doi:10.4238/2015.march.27.18

Cited by 10 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies using overexpression systems also supported the role of JA in the plant salt response and tolerance. Heterologous overexpression of peanut (Arachis hypogaea) AhAOC, which is responsible for JA biosynthesis, increased cellular JA levels by 1.5-fold in rice [110]. The transgenic rice exhibited increased expression levels of stress-responsive genes such as OsLEA3, OsMYB2, and OsONAC045, and formed longer roots than wild-type plants under salt stress conditions [110].…”

Section: Ja and Salt Stressmentioning

confidence: 99%

“…Heterologous overexpression of peanut (Arachis hypogaea) AhAOC, which is responsible for JA biosynthesis, increased cellular JA levels by 1.5-fold in rice [110]. The transgenic rice exhibited increased expression levels of stress-responsive genes such as OsLEA3, OsMYB2, and OsONAC045, and formed longer roots than wild-type plants under salt stress conditions [110]. This finding was consistent with the result that transgenic Arabidopsis heterologously overexpressing the maize JA biosynthetic gene ZmOPR1 showed improved salt tolerance compared with wild-type plants [111].…”

Section: Ja and Salt Stressmentioning

confidence: 99%

See 1 more Smart Citation

Jasmonic Acid in Plant Abiotic Stress Tolerance and Interaction with Abscisic Acid

et al. 2021

View full text Add to dashboard Cite

The phytohormone jasmonic acid (JA), a cyclopentane fatty acid, mediates plant responses to abiotic stresses. Abiotic stresses rapidly and dynamically affect JA metabolism and JA responses by upregulating the expression of genes involved in JA biosynthesis and signaling, indicating that JA has a crucial role in plant abiotic stress responses. The crucial role of JA has been demonstrated in many previous studies showing that JA response regulates various plant defense systems, such as removal of reactive oxygen species and accumulation of osmoprotectants. Furthermore, increasing evidence shows that plant tolerance to abiotic stresses is linked to the JA response, suggesting that abiotic stress tolerance can be improved by modulating JA responses. In this review, we briefly describe the JA biosynthetic and signaling pathways and summarize recent studies showing an essential role of JA in plant responses and tolerance to a variety of abiotic stresses, such as drought, cold, salt, and heavy metal stress. Additionally, we discuss JA crosstalk with another key stress hormone, abscisic acid, in plant abiotic stress responses.

show abstract

Section: Ja and Salt Stressmentioning

confidence: 99%

Section: Ja and Salt Stressmentioning

confidence: 99%

Jasmonic Acid in Plant Abiotic Stress Tolerance and Interaction with Abscisic Acid

et al. 2021

View full text Add to dashboard Cite

show abstract

“…For instance, drought-induced peanut AhNAC2 overexpressed transgenic Arabidopsis exhibited enhanced tolerance to drought and salinity stress (Liu et al, 2011 ). Overexpression of peanut allene oxide cyclase (AhAOC) in rice conferred tolerance against salinity (Liu et al, 2015 ). However, the researches about salt stress response genes in peanut are rare up to now.…”

Section: Introductionmentioning

confidence: 99%

Identification of Metabolites and Transcripts Involved in Salt Stress and Recovery in Peanut

et al. 2018

View full text Add to dashboard Cite

HIGHLIGHTS Metabolites and transcripts related to plant physiology in salt stress conditions, especially to the recovery process were disclosed in peanut.Peanut (Arachis hypogaea L.) is considered as a moderately salt-sensitive species and thus soil salinity can be a limiting factor for peanut cultivation. To gain insights into peanut plant physiology in response to salt stress and alleviation, we comprehensively characterized leaf relative electrolyte leakage (REC), photosynthesis, leaf transpiration, and metabolism of plants under salt stress and plants that were subjected to salt stress followed by salt alleviation period. As expected, we found that REC levels were higher when plants were subjected to salt stress compared with the untreated plants. However, in contrast to expectations, REC was even higher compared with salt treated plants when plants were transferred from salt stress to standard conditions. To decipher REC variation in response to salt stress, especial during the recovery, metabolite, and transcript variations were analyzed by GC/MS and RNA-seq method, respectively. Ninety two metabolites, among total 391 metabolites identified, varied in response to salt and 42 metabolites responded to recovery specially. Transcriptomics data showed 1,742 in shoots and 3,281 in roots transcript varied in response to salt stress and 372 in shoots and 1,386 transcripts in roots responded specifically to recovery, but not salt stress. Finally, 95 transcripts and 1 metabolite are indicated as candidates involved in REC, photosynthesis, transpiration, and Na+ accumulation variation were revealed by using the principal component analysis (PCA) and correlation analysis. This study provides valuable information on peanut response to salt stress and recovery and may inspire further study to improve salt tolerance in peanut germplasm innovation.

show abstract

“…In Arabidopsis , JMT catalyzes the conversion of JA into MeJA and participates in JA-responsive defense mechanisms ( Seo et al., 2001 ). In Arachis hypogaea , AhAOC can be induced by JA to enhance plant resistance to stress ( Liu et al., 2015 ). Tobacco NaLOX3 expression is induced by the fungal initiator ergosterol ( Dadakova et al., 2013 ), while Pisum sativum L enhances waterlogging tolerance by regulating linoleate Ps9S-LOX5 gene expression ( Zaman et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

The heat shock factor GhHSFA4a positively regulates cotton resistance to Verticillium dahliae

et al. 2022

View full text Add to dashboard Cite

Heat shock factors (HSFs) play a crucial role in the environmental stress responses of numerous plant species, including defense responses to pathogens; however, their role in cotton resistance to Verticillium dahliae remains unclear. We have previously identified several differentially expressed genes (DEGs) in Arabidopsis thaliana after inoculation with V. dahliae. Here, we discovered that GhHSFA4a in Gossypium hirsutum (cotton) after inoculation with V. dahliae shares a high identity with a DEG in A. thaliana in response to V. dahliae infection. Quantitative real-time PCR (qRT-PCR) analysis indicated that GhHSFA4a expression was rapidly induced by V. dahliae and ubiquitous in cotton roots, stems, and leaves. In a localization analysis using transient expression, GhHSFA4a was shown to be localized to the nucleus. Virus-induced gene silencing (VIGS) revealed that downregulation of GhHSFA4a significantly increased cotton susceptibility to V. dahliae. To investigate GhHSFA4a-mediated defense, 814 DEGs were identified between GhHSFA4a-silenced plants and controls using comparative RNA-seq analysis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were enriched in “flavonoid biosynthesis”, “sesquiterpenoid and triterpenoid biosynthesis”, “linoleic acid metabolism” and “alpha-linolenic acid metabolism”. The expression levels of marker genes for these four pathways were triggered after inoculation with V. dahliae. Moreover, GhHSFA4a-overexpressing lines of A. thaliana displayed enhanced resistance against V. dahliae compared to that of the wild type. These results indicate that GhHSFA4a is involved in the synthesis of secondary metabolites and signal transduction, which are indispensable for innate immunity against V. dahliae in cotton.

show abstract

Cloning and characterization of peanut allene oxide cyclase gene involved in salt-stressed responses

Cited by 10 publications

References 25 publications

Jasmonic Acid in Plant Abiotic Stress Tolerance and Interaction with Abscisic Acid

Jasmonic Acid in Plant Abiotic Stress Tolerance and Interaction with Abscisic Acid

Identification of Metabolites and Transcripts Involved in Salt Stress and Recovery in Peanut

The heat shock factor GhHSFA4a positively regulates cotton resistance to Verticillium dahliae

Contact Info

Product

Resources

About