Cloning and characterization of polyA^-RNA transcripts encoded by activated B1-like retrotransposons in mouse erythroleukemia MEL cells exposed to methylation inhibitors

Possible interaction between B1 retrotransposon‐containing sequences and βmajor globin gene transcriptional activation during MEL cell erythroid differentiation

“…luciferase gene) in haemopoietic cells either alone, or in cooperation with the element HS-2 of globin gene LCR region. We also showed in another study that nonpoly(A) 2 RNA transcripts isolated and cloned from the cytoplasm of MEL cells exposed to both differentiation inducers and methylation inhibitors have a high structural similarity with the B1 retrotransposon element located within the B1-559 region (Tezias et al, 2011). The fact that the B1-559 DNA fragment described here exhibits potential promoter-like activity to drive luciferase gene transient expression in haemopoietic cells in cooperation with HS-2 suggests that the identified 3 consensus sequences for GATA-1, AP-1/NF-E2 and EKLF located within this fragment may contribute to transcriptional activation.…”

“…Four polyA 2 RNAs of 448, 391, 174 and 151 nt were cloned and characterized as described in Tezias et al (2011). Four polyA 2 RNAs of 448, 391, 174 and 151 nt were cloned and characterized as described in Tezias et al (2011).…”

“…The data obtained thus far indicate that DNA sequences located at distant 39-end flanking b major globin gene region may encode RNA transcripts accumulated upon exposure of MEL cells to methylation inhibitors. Four polyA 2 RNAs of 448, 391, 174 and 151 nt were cloned and characterized as described in Tezias et al (2011). Keeping in mind that this region contains consensus sequences recognized by the erythroid-specific transcription factors GATA-1, AP-1/NF-E2 and EKLF in the vicinity of B1 repeat element (Vizirianakis and Tsiftsoglou, 2005), assessment of the functional characterization of this particular DNA region was considered important.…”

Roles of rpoS‐activating small RNAs in pathways leading to acid resistance of Escherichia coli

“…Northern blot analysis was carried out as described (Tezias et al. ). Briefly, 10 μ g of total RNAs were fractionated on 7 mol/L urea, 5% PAGE, and electrotransferred onto a Hybond™‐XL membrane (Amersham Biosciences, Little Chalfont, UK).…”

“…Total cellular RNAs were extracted from culture at the desired time points by acidic hot phenol method, as described previously (Kim et al 1996). Northern blot analysis was carried out as described (Tezias et al 2012). Briefly, 10 lg of total RNAs were fractionated on 7 mol/L urea, 5% PAGE, and electrotransferred onto a Hybond TM -XL membrane (Amersham Biosciences, Little Chalfont, UK).…”