Cloning and characterization of two K+ transporters of Debaryomyces hansenii

Prista, Catarina; González‐Hernández, Juan Carlos; Ramos, José; Loureiro-Dias, Maria C.

doi:10.1099/mic.0.2007/006080-0

Cited by 35 publications

(32 citation statements)

References 56 publications

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“…Both S. occidentalis and D. hansenii possess a HAK-type transporter as well [42,90]. For S. occidentalis, it has been proposed that SoTrk1 plays an important role in the control of the membrane potential and mediates K ?…”

Section: Trk Transporters In S Cerevisiaementioning

confidence: 99%

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Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family

Corratgé-Faillie

Jabnoune

Zimmermann

et al. 2010

Cell. Mol. Life Sci.

212

172

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Bacterial Trk and Ktr, fungal Trk and plant HKT form a family of membrane transporters permeable to K(+) and/or Na(+) and characterized by a common structure probably derived from an ancestral K(+) channel subunit. This transporter family, specific of non-animal cells, displays a large diversity in terms of ionic permeability, affinity and energetic coupling (H(+)-K(+) or Na(+)-K(+) symport, K(+) or Na(+) uniport), which might reflect a high need for adaptation in organisms living in fluctuating or dilute environments. Trk/Ktr/HKT transporters are involved in diverse functions, from K(+) or Na(+) uptake to membrane potential control, adaptation to osmotic or salt stress, or Na(+) recirculation from shoots to roots in plants. Structural analyses of bacterial Ktr point to multimeric structures physically interacting with regulatory subunits. Elucidation of Trk/Ktr/HKT protein structures along with characterization of mutated transporters could highlight functional and evolutionary relationships between ion channels and transporters displaying channel-like features.

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Section: Trk Transporters In S Cerevisiaementioning

confidence: 99%

“…For D. hansenii, which lives in marine habitats and thus in presence of K ? concentrations in the mM range, both DhTrk1 and DhHAK have been suggested to behave as low-affinity transporters [90].…”

Section: Trk Transporters In S Cerevisiaementioning

confidence: 99%

Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family

Corratgé-Faillie

Jabnoune

Zimmermann

et al. 2010

Cell. Mol. Life Sci.

212

172

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“…Subsequently, HAK1-orthologous genes have been found in many other organisms, such as the nonconventional yeasts D. hansenii (220) and Hansenula polymorpha (J. M. Siverio, personal communication), the micelial fungus N. crassa (104), the moss Physcomitrella patens (84), and higher plants (259,276 ϩ ions, is quantitatively conserved between C. albicans and S. cerevisiae. However, the secondary function, chloride efflux channeling, is present but poorly conserved between the two species, being highly variant with respect to activation velocity, amplitude, flickering (channel-like) behavior, pH dependence, and inhibitor sensitivity (170).…”

mentioning

confidence: 99%

“…However, an interesting approach was followed by Prista and coworkers (221), who showed that transformation with genes from D. hansenii can improve the salt tolerance of a wild-type S. cerevisiae strain. Concerning K ϩ transporters, the most important novelty compared to the situation in S. cerevisiae or S. pombe is the existence of a single TRK gene and an additional and completely different transporter encoded by D. hansenii HAK1 (DhHAK1) (220).…”

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confidence: 99%

Alkali Metal Cation Transport and Homeostasis in Yeasts

Ariño

Ramos

Sychrová

2010

Microbiol Mol Biol Rev

261

299

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SUMMARY The maintenance of appropriate intracellular concentrations of alkali metal cations, principally K+ and Na+, is of utmost importance for living cells, since they determine cell volume, intracellular pH, and potential across the plasma membrane, among other important cellular parameters. Yeasts have developed a number of strategies to adapt to large variations in the concentrations of these cations in the environment, basically by controlling transport processes. Plasma membrane high-affinity K+ transporters allow intracellular accumulation of this cation even when it is scarce in the environment. Exposure to high concentrations of Na+ can be tolerated due to the existence of an Na+, K+-ATPase and an Na+, K+/H+-antiporter, which contribute to the potassium balance as well. Cations can also be sequestered through various antiporters into intracellular organelles, such as the vacuole. Although some uncertainties still persist, the nature of the major structural components responsible for alkali metal cation fluxes across yeast membranes has been defined within the last 20 years. In contrast, the regulatory components and their interactions are, in many cases, still unclear. Conserved signaling pathways (e.g., calcineurin and HOG) are known to participate in the regulation of influx and efflux processes at the plasma membrane level, even though the molecular details are obscure. Similarly, very little is known about the regulation of organellar transport and homeostasis of alkali metal cations. The aim of this review is to provide a comprehensive and up-to-date vision of the mechanisms responsible for alkali metal cation transport and their regulation in the model yeast Saccharomyces cerevisiae and to establish, when possible, comparisons with other yeasts and higher plants.

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“…También se han encontrado transportadores del tipo TRK en Neurospora crassa (Haro et al, 1999), Schwanniomyces occidentalis (Banuelos et al, 2000) y Debaryomyces hansenii (Prista et al, 2007). En estas especies, además de los sistemas de transporte del tipo TRK, también están presentes otro tipo de transportadores de potasio, los HAK (HighAffinity K + -transporter), que no existen en S. cerevisiae.…”

Section: El Transporte De Potasio En Saccharomyces Cerevisiaeunclassified

Estudio de los mecanismos de la regulación de la homeostasis iónica: Análisis fisiológico y transcriptómico del mutante hal4hal5 de Saccharomyces cerevisiae.

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Cloning and characterization of two K+ transporters of Debaryomyces hansenii

Abstract: Two genes from the halotolerant yeast Debaryomyces hansenii were cloned, DhTRK1 and DhHAK1. These genes encode K + transporters with sequence similarities to the TRK and HAK transporters from Debaryomyces occidentalis and Candida albicans.

Cited by 35 publications

References 56 publications

Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family

Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family

Alkali Metal Cation Transport and Homeostasis in Yeasts

Estudio de los mecanismos de la regulación de la homeostasis iónica: Análisis fisiológico y transcriptómico del mutante hal4hal5 de Saccharomyces cerevisiae.

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