Cloning and comparative characterization of three distinct nucleopolyhedroviruses isolated from the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) in Japan

Kamiya, Katsumi; Zhu, Jiang; Murata, Masanori; Laviña-Caoili, Barbara A.; Ikeda, Motoko; Kobayashi, Michihiro; Kawamura, Satoshi

doi:10.1016/j.biocontrol.2004.04.003

Cited by 30 publications

(32 citation statements)

References 29 publications

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“…The REN pattern of SpltNPV-Pak-BNG corresponds to the REN patterns of SpltNPV-Type-B (Kamiya et al, 2004) and Japanese SpltNPVs (Takatsuka et al, 2003;Takatsuka, Okuno, Nakai, & Kunimi, 2016) on the basis of visual inspection of the REN digests. On the other hand, the SpltNPV-G1 REN profiles are very different from SpltNPV-Pak-BNG, but they appear closely related to SpltNPVType-A (Kamiya et al, 2004) and SpliNPV- AN-1956(Breitenbach et al, 2013. Sequence analysis of the baculovirus core genes polyhedrin, DNApol, lef-6 and pif-2 (Table 6) supports this observation.…”

Section: Discussionmentioning

confidence: 99%

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Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Ali¹,

Werf

Vlak

2017

Biocontrol Science and Technology

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Spodoptera litura is an emerging insect pest in a wide range of crops worldwide. The insect is difficult to control because of resistance development to synthetic insecticides and emerging resistance to Bacillus thuringiensis toxins. Therefore, there is a need to develop biological control agents, preferably from an indigenous source to avoid risks associated with the importation of exotic natural antagonists. A Pakistani isolate of S. litura nucleopolyhedrovirus (SpltNPV, Baculoviridae), SpltNPV-Pak-BNG, was obtained from the field and characterized biologically and genetically, and compared to a SpltNPV reference isolate, SpltNPV-G1, thought to be of Chinese origin. The dose-mortality response (LD 50 ) of SpltNPVPak-BNG was not significantly different from that of the reference isolate SpltNPV-G1, but the time-to-death (LT 50 ) was significantly shorter for SpltNPV-Pak-BNG than for SpltNPV-G1. DNA restriction enzyme profiling indicated that SpltNPV-Pak-BNG and SpltNPV-G1 are different viruses. Sequence analysis of 'ORF24', specific for SpltNPV (and S. littoralis NPV as ORF21), and the conserved baculovirus core genes polyhedrin, DNApol, pif-2 and lef-8 confirmed that this was indeed the case and that SpltNPV-Pak-BNG is a genuine SpltNPV variant, whereas the SpltNPV-G1 isolate we used is, in fact, a SpliNPV variant, renamed to SpliNPV-G1. The newly isolated SpltNPV-Pak-BNG has the potential for development as a biocontrol agent of S. litura in Pakistan.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

“…It has been shown that large genetic variation exists between SpltNPV and a SpliNPV field isolates (Kamiya et al, 2004;Takatsuka et al, 2003Takatsuka et al, , 2016 and that biological variation between isolates exists (Takatsuka et al, 2016). So, it is worthwhile to obtain further SpltNPV isolates Table 6.…”

Section: Discussionmentioning

confidence: 99%

Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Ali¹,

Werf

Vlak

2017

Biocontrol Science and Technology

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“…Genome sequencing and phylogenetic, evolutionary and selection analyses would be necessary to support this hypothesis. Phenotypic differences are derived from changes at the genome level (40,41) and are important for virus adaptation and survival (8,42). The major source of intraspecific variability among genotypes can be found in homologous-repeat regions (hrs) (5), the bro gene family (5,11,37), and the hoar gene (38).…”

Section: Discussionmentioning

confidence: 99%

A Chrysodeixis chalcites Single-Nucleocapsid Nucleopolyhedrovirus Population from the Canary Islands Is Genotypically Structured To Maximize Survival

Bernal

Simón

Williams

et al. 2013

Appl Environ Microbiol

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A Chrysodeixis chalcites single-nucleocapsid nucleopolyhedrovirus wild-type isolate from the Canary Islands, Spain, named ChchSNPV-TF1 (ChchTF1-wt), appears to have great potential as the basis for a biological insecticide for control of the pest. An improved understanding of the genotypic structure of this wild-type strain population should facilitate the selection of genotypes for inclusion in a bioinsecticidal product. Eight genetically distinct genotypes were cloned in vitro: ChchTF1-A to ChchTF1-H. Quantitative real-time PCR (qPCR) analysis confirmed that ChchTF1-A accounted for 36% of the genotypes in the wild-type population. In bioassays, ChchTF1-wt occlusion bodies (OBs) were significantly more pathogenic than any of the component single-genotype OBs, indicating that genotype interactions were likely responsible for the pathogenicity phenotype of wild-type OBs. However, the wild-type population was slower killing and produced higher OB yields than any of the single genotypes alone. These results strongly suggested that the ChchTF1-wt population is structured to maximize its transmission efficiency. Experimental OB mixtures and cooccluded genotype mixtures containing the most abundant and the rarest genotypes, at frequencies similar to those at which they were isolated, revealed a mutualistic interaction that restored the pathogenicity of OBs. In OB and cooccluded mixtures containing only the most abundant genotypes, ChchTF1-ABC, OB pathogenicity was even greater than that of wild-type OBs. The ChchTF1-ABC cooccluded mixture killed larvae 33 h faster than the wild-type population and remained genotypically and biologically stable throughout five successive passages in vivo. In conclusion, the ChchTF1-ABC mixture shows great potential as the active ingredient of a bioinsecticide to control C. chalcites in the Canary Islands.

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“…Restriction endonuclease (REN) analyses of the viral genome have shown that these field isolates consist of various genotypic variants. Analysis of clonal NPV isolates has further shown that such genotypic variations distinguishable by REN analysis are reflected frequently in their phenotypic variations of virulence and productivity in both individual insects and cell cultures [14][15][16][17][18][19], thus indicating that clonal NPV isolates provide a basis for the selection of high-potential variants for development of efficacious microbial insecticides.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic sequence analysis of novel Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Kenya and three other previously sequenced Helicoverpa spp. NPVs

et al. 2009

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A newly cloned Helicoverpa armigera nucleopolyhedrovirus (HearNPV) from Kenya, HearNPV-NNg1, has a higher insecticidal activity than HearNPV-G4, which also exhibits lower insecticidal activity than HearNPV-C1. In the search for genes and/or nucleotide sequences that might be involved in the observed virulence differences among Helicoverpa spp. NPVs, the entire genome of NNg1 was sequenced and compared with previously sequenced genomes of G4, C1 and Helicoverpa zea single-nucleocapsid NPV (Hz). The NNg1 genome was 132,425 bp in length, with a total of 143 putative open reading frames (ORFs), and shared high levels of overall amino acid and nucleotide sequence identities with G4, C1 and Hz. Three NNg1 ORFs, ORF5, ORF100 and ORF124, which were shared with C1, were absent in G4 and Hz, while NNg1 and C1 were missing a homologue of G4/Hz ORF5. Another three ORFs, ORF60 (bro-b), ORF119 and ORF120, and one direct repeat sequence (dr) were unique to NNg1. Relative to the overall nucleotide sequence identity, lower sequence identities were observed between NNg1 hrs and the homologous hrs in the other three Helicoverpa spp. NPVs, despite containing the same number of hrs located at essentially the same positions on the genomes. Differences were also observed between NNg1 and each of the other three Helicoverpa spp. NPVs in the diversity of bro genes encoded on the genomes. These results indicate several putative genes and nucleotide sequences that may be responsible for the virulence differences observed among Helicoverpa spp., yet the specific genes and/or nucleotide sequences responsible have not been identified.

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Cloning and comparative characterization of three distinct nucleopolyhedroviruses isolated from the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) in Japan

Cited by 30 publications

References 29 publications

Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

A Chrysodeixis chalcites Single-Nucleocapsid Nucleopolyhedrovirus Population from the Canary Islands Is Genotypically Structured To Maximize Survival

Comparative genomic sequence analysis of novel Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Kenya and three other previously sequenced Helicoverpa spp. NPVs

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