Cloning and expression analysis of <i>takeout/JHBP</i> family genes of silkworm, <i>Bombyx mori</i>

蘇, 智慧; Emi, Aki; Mita, Kazuei; Takeda, Makio; Fujiwara, Yoshihiro

doi:10.1111/j.1365-2583.2006.00612.x

Cited by 40 publications

(56 citation statements)

References 27 publications

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“…Phylogenetic analyses of Lepidoptera JHPB/To protein sequences show that the To proteins fall into six distinct lineages, with the JHBPs forming an outlier group within the superfamily ( Fig. 1) (7,11). Five of the six Lepidoptera To lineages (Lep1, Lep3, Lep4, Lep5, and Lep6) are supported by high bootstrap values.…”

Section: Identification Of Four New Takeout Family Members-mentioning

confidence: 95%

“…At least 80 To genes have now been identified from seven orders of insect, including 20 from D. melanogaster, but few have been characterized in any detail. Due to the localization and/or expression profiles of their transcripts, some are proposed to have roles in chemosensory perception (5)(6)(7)(8), whereas others are thought to be involved in binding terpenoids (9), including juvenile hormones (JH) (10 -15).…”

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confidence: 99%

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Crystal Structure of Epiphyas postvittana Takeout 1 with Bound Ubiquinone Supports a Role as Ligand Carriers for Takeout Proteins in Insects

Hamiaux

Stanley

Greenwood

et al. 2009

Journal of Biological Chemistry

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Takeout (To) proteins are found exclusively in insects and have been proposed to have important roles in various aspects of their physiology and behavior. Limited sequence similarity with juvenile hormone-binding proteins (JHBPs), which specifically bind and transport juvenile hormones in Lepidoptera, suggested a role for To proteins in binding hydrophobic ligands. We present the first crystal structure of a To protein, EpTo1 from the light brown apple moth Epiphyas postvittana, solved in-house by the single-wavelength anomalous diffraction technique using sulfur anomalous dispersion, and refined to 1.3 Å resolution. EpTo1 adopts the unusual ␣/␤-wrap fold, seen only for JHBP and several mammalian lipid carrier proteins, a scaffold tailored for the binding and/or transport of hydrophobic ligands. EpTo1 has a 45 Å long, purely hydrophobic, internal tunnel that extends for the full length of the protein and accommodates a bound ligand. The latter was shown by mass spectrometry to be ubiquinone-8 and is probably derived from Escherichia coli. The structure provides the first direct experimental evidence that To proteins are ligand carriers; gives insights into the nature of endogenous ligand(s) of EpTo1; shows, by comparison with JHBP, a basis for different ligand specificities; and suggests a mechanism for the binding/release of ligands. Takeout (To) 3 family have been proposed to have important roles in various aspects of insect physiology and behavior and as such may represent novel targets for insect control strategies. The original member, To, was discovered in Drosophila melanogaster as a clock-regulated gene, acting as a molecular link between circadian rhythms and feeding behavior (1, 2). In addition, To expression is regulated by the somatic sex determination pathway and affects male courtship behavior (3, 4). To has a predicted secretion signal (1) and is secreted in the hemolymph of adult males (4). At least 80 To genes have now been identified from seven orders of insect, including 20 from D. melanogaster, but few have been characterized in any detail. Due to the localization and/or expression profiles of their transcripts, some are proposed to have roles in chemosensory perception (5-8), whereas others are thought to be involved in binding terpenoids (9), including juvenile hormones (JH) (10 -15). Members of theTo proteins share limited sequence similarity and a conserved N-terminal disulfide bond with juvenile hormone-binding proteins (JHBPs). These proteins specifically bind and transport JH to target tissues in the Lepidoptera, further suggesting a role for To proteins in binding hydrophobic ligands (2). There are, however, two major differences between the families: a second disulfide bond in the JHBPs, not present in To; and the presence of two conserved C-terminal sequence motifs that are unique to the To family (2). Very recently, the structure of JHBP from the moth Galleria mellonella (GmJHBP) has been solved, revealing an unusual fold in which a highly curved antiparallel ␤-sheet wraps around a...

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Section: Identification Of Four New Takeout Family Members-mentioning

confidence: 95%

mentioning

confidence: 99%

Crystal Structure of Epiphyas postvittana Takeout 1 with Bound Ubiquinone Supports a Role as Ligand Carriers for Takeout Proteins in Insects

Hamiaux

Stanley

Greenwood

et al. 2009

Journal of Biological Chemistry

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“…Indeed, to mRNA is expressed in structures related to feeding such as cardia, crop (a dilatation from the caudal end of the oesophagus, serving as a food reservoir), head fat body and antennae (Dauwalder et al, 2002;Sarov-Blat et al, 2000). Takeout (TO) expression is also induced by starvation (Sarov-Blat et al, 2000) similarly to related genes in Bombyx mori (Saito et al, 2006). Finally, to mutated flies (to 1 ) display a reduced resistance to starvation.…”

Section: Introductionmentioning

confidence: 99%

Regulation of feeding behaviour and locomotor activity bytakeoutinDrosophila

Meunier

Belgacem

Martin

2007

Journal of Experimental Biology

140

143

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SUMMARY The hormonal regulation of feeding behaviour is well known in vertebrates,whereas it remains poorly understood in insects. Here, we report that the takeout gene is an essential component of nutritional homeostasis in Drosophila. takeout encodes a putative juvenile hormone (JH)binding protein and has been described as a link between circadian rhythm and feeding behaviour. However, the physiological role of takeout and its putative link to JH remain unknown. In this study, we show that takeout (to1) flies failed to adapt their food intake according to food availability and that most defects could be genetically rescued. When food is abundant, to1 are hyperphagic, yielding to hypertrophy of the fat body. When food reappears after a starvation period, to1 flies do not increase their food intake as much as wild-type flies. This defect in food intake regulation is partly based on the action of Takeout on taste neurons, because the sensitivity of to1 gustatory neurons to sugars does not increase after starvation, as in wild-type neurons. This lack of regulation is also evident at the locomotor activity, which normally increases during starvation, a behaviour related to food foraging. In addition, to1 flies lack sexual dimorphism of locomotor activity,which has previously been linked to the JH circulating level. Moreover,application of the JH analog methoprene rescues the phenotype. These results suggest that takeout plays a central role as a feeding regulator and may act by modulating the circulating JH level.

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“…This gene, originally named as e96h-0303 of the family, was found in the expressed sequence tags generated from eggs of B. mori (Saito et al, 2006). The expression pattern of this gene at other developmental stages and/or tissues is not known.…”

Section: Discussionmentioning

confidence: 97%

“…The real-time RT-PCR reactions were performed using the Lightcycler-Faststart DNA master SYBR green I PCR kit in a Lightcycler 1.2 real-time PCR System (Roche, Basel, Switzerland) according to the operator's manual. Sequence-specific oligonucleotide primers designed for a member of the takeout/Juvenile hormone-binding protein family gene (e96h-0303 described in Saito et al, 2006, and shortened as JHBP0303), and three AMPs were used to yield approximately 100-250 base pair (bp) sequences. All primers used in this study were designed with the Primer Premier 5.0 software (Premier, Canada) and synthesized at Invitrogen.…”

Section: Real-time Reverse-transcription Coupled With Polymerase Chaimentioning

confidence: 99%

Gene expression profiling during early response to injury and microbial challenges in the silkworm, Bombyx mori

Liu

Ling

2009

Arch Insect Biochem Physiol

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To identify Bombyx mori genes involved in the early response to injury and microbial challenge, we performed genome-wide gene expression-profiling experiments using oligonucleotide DNA microarrays. Of approximately 23,000 genes examined, 465 displayed changes in mRNA expression levels. Of these, 306 were induced and 159 were repressed in response to injury (injection with phosphate buffer saline) or challenges by Gram-negative (Serratia marcescens), Gram-positive bacteria (Staphylococcus aureus), or fungus (Beauveria bassiana). Many of these differentially expressed genes can be assigned to specific functional groups of the innate immune response, including recognition, signaling, melanization and coagulation, and antimicrobial peptides. Seventeen percent of differentially expressed genes encode proteins with no obvious similarity to known functional domains. Of particular interest is a member of the juvenile hormone-binding protein family, which was highly induced by both injury and microbial challenges. The possible role of juvenile hormone in innate immunity is discussed.

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Cloning and expression analysis of takeout/JHBP family genes of silkworm, Bombyx mori

Cited by 40 publications

References 27 publications

Crystal Structure of Epiphyas postvittana Takeout 1 with Bound Ubiquinone Supports a Role as Ligand Carriers for Takeout Proteins in Insects

Crystal Structure of Epiphyas postvittana Takeout 1 with Bound Ubiquinone Supports a Role as Ligand Carriers for Takeout Proteins in Insects

Regulation of feeding behaviour and locomotor activity bytakeoutinDrosophila

Gene expression profiling during early response to injury and microbial challenges in the silkworm, Bombyx mori

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