Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica

Ma, Ke; Chen, Guangwen; Lou, Hao; Fei, Li-Na

doi:10.2478/s11756-009-0158-8

Cited by 10 publications

(10 citation statements)

References 37 publications

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“…Djhsp70 is an inducible gene, significantly up-regulated in response to temperature changes, amputation and starvation (Ma et al, 2009). Our whole mount in situ hybridizations demonstrated that Djhsp70 transcripts are widely distributed along the planarian body and up-regulated in the regenerative blastema (Fig.…”

Section: Hsp70 Genesmentioning

confidence: 74%

“…Although our in silico search revealed that several hsp70-related genes are present in the planarian genome (not shown), only two different members of this family have been characterized so far: Djhsp70 and Djmot (Conte et al, 2009;Ma et al, 2009). Djhsp70 is an inducible gene, significantly up-regulated in response to temperature changes, amputation and starvation (Ma et al, 2009).…”

Section: Hsp70 Genesmentioning

confidence: 94%

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Stem cell protection mechanisms in planarians: the role of some heat shock genes

Isolani¹,

Conte²,

Deri³

et al. 2012

Int. J. Dev. Biol.

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Planarians contain a large population of stem cells, named neoblasts, and use these for continuous turnover of all cell types. In addition, thanks to the amazing flexibility of these cells, planarians respond well to the effects of stressful situations, for example activating regeneration after trauma. How neoblasts respond to stress and support continuous proliferation, maintaining long-term stability, is still an open question. Heat shock proteins (HSPs) are a complex protein family with key roles in maintaining protein homeostasis, as well as in apoptosis and growthrelated processes. We recently characterized some planarian homologs of hsp genes that are highly expressed in mammalian stem cells, and observed that some of them are critical for neoblast survival/maintenance. The results of these studies support the notion that some HSPs play crucial roles in the modulation of pathways regulating stem cell activity, regeneration and tissue repair. In this review we compare the evidence available for planarian hsp genes and focus on questions emerging from these results.

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Section: Hsp70 Genesmentioning

confidence: 74%

Section: Hsp70 Genesmentioning

confidence: 94%

Stem cell protection mechanisms in planarians: the role of some heat shock genes

Isolani¹,

Conte²,

Deri³

et al. 2012

Int. J. Dev. Biol.

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“…Several authors have proposed the use of the absence of introns as a criterion for recognizing the inducible hsp70 isoforms (Günther & Walter ; Ma et al . ). However, this criterion should be used with caution given that an intronless cognate form of hsp70 was recently described in the giant freshwater prawn Macrobrachium rosenbergii (Crustacea: Decapoda) (Liu et al .…”

Section: Discussionmentioning

confidence: 97%

Expression patterns and organization of the hsp70 genes correlate with thermotolerance in two congener endemic amphipod species (Eulimnogammarus cyaneus and E. verrucosus) from Lake Baikal

et al. 2013

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We studied various aspects of heat-shock response with special emphasis on the expression of heat-shock protein 70 (hsp70) genes at various levels in two congener species of littoral endemic amphipods (Eulimnogammarus cyaneus and E. verrucosus) from Lake Baikal which show striking differences in their vertical distribution and thermal tolerance. Although both the species studied demonstrate high constitutive levels of Hsp70, the thermotolerant E. cyaneus exhibited a 5-fold higher basal level of Hsp70 proteins under normal physiological conditions (7 °C) and significantly lower induction of Hsp70 after temperature elevation compared with the more thermosensitive E. verrucosus. We isolated the hsp70 genes from both species and analysed their sequences. Two isoforms of the cytosolic Hsp70/Hsc70 proteins were detected in both species under normal physiological conditions and encoded by two distinct hsp/hsc70 family members. While both Hsp70 isoforms were synthesized without heat shock, only one of them was induced by temperature elevation. The observed differences in the Hsp70 expression patterns, including the dynamics of Hsp70 synthesis and threshold of induction, suggest that the increased thermotolerance in E. cyaneus (compared with E. verrucosus) is associated with a complex structural and functional rearrangement of the hsp70 gene family and favoured the involvement of Hsp70 in adaptation to fluctuating thermal conditions. This study provides insights into the molecular mechanisms underlying the thermal adaptation of Baikal amphipods and represents the first report describing the structure and function of the hsp70 genes of endemic Baikal species dwelling in thermally contrasting habitats.

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“…However, the acute thermal stress is lethal to planarians. To investigate heat-shock response, most studies focused on the expression pattern of heatshock proteins (Ma et al 2009(Ma et al , 2012Manchado et al 2008). In the present study, we selected ALP as an indicator to evaluate the effects of thermal stress on the plasma membrane.…”

Section: Resultsmentioning

confidence: 99%

Changes of alkaline phosphatase activity in response to different stressors in planarian Dugesia japonica

Chen

Liu

2013

Biologia

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The aims of this work are to provide some properties of alkaline phosphatase (ALP) in the planarian Dugesia japonica and detect its activity in response to different stressors, as well as to introduce renatured SDS-PAGE to study enzyme activity in planarians. Our results indicate that ALPs in planarians are mainly membrane-bound form, identified as three mainly enzyme-bands (approximately MW 260 kD, 180 kD, 160 kD, respectively). Under our experimental conditions, ALPs activity had no apparent changes in response to low concentration of Hg • C for 2 days) inactivated ALP activity. Interestingly, ALP and other cytosolic phosphatases (MW from ∼45 kD to ∼60 kD) activity increased noticeably during the early stage of planarians regeneration, which may be involved in cell proliferation and differentiation. Contrary to regeneration, prolonged starvation suppressed ALP activity. The above findings provide valuable information about the role of ALP in planarian regeneration and for its use as an indicator in ecotoxicology.

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Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica

Cited by 10 publications

References 37 publications

Stem cell protection mechanisms in planarians: the role of some heat shock genes

Stem cell protection mechanisms in planarians: the role of some heat shock genes

Expression patterns and organization of the hsp70 genes correlate with thermotolerance in two congener endemic amphipod species (Eulimnogammarus cyaneus and E. verrucosus) from Lake Baikal

Changes of alkaline phosphatase activity in response to different stressors in planarian Dugesia japonica

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