Cloning and expression analysis of a mouse gene related to Drosophila odd-skipped

So, Po Lin; Danielian, Paul S.

doi:10.1016/s0925-4773(99)00058-1

Cited by 69 publications

(72 citation statements)

References 14 publications

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“…Odd homologous genes have been cloned and characterised from several other species, including Caenorhabditis elegans (Buckley et al, 2004), mouse (So and Danielian, 1999;Lan et al, 2001) and man (Debeer et al, 2002;Katoh, 2002).…”

Section: Resultsmentioning

confidence: 99%

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Comparative expression pattern of Odd-skipped related genes Osr1 and Osr2 in chick embryonic development

Stricker

Brieske

Haupt

et al. 2006

Gene Expression Patterns

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Odd-skipped genes encode zinc-finger transcription factors with widespread roles in embryonic development. In Drosophila, oddskipped acts as a pair-rule gene, while its orthologous gene in Caenorhabditis elegans is involved in gut development. In mammals two paralogs exist, Osr1 and Osr2, with functions described in heart and urogenital, and in secondary palate development, respectively. As the chicken embryo is a widely used system for analysing gene function in vivo, we determined the expression pattern of the two chicken orthologues, cOsr1 and cOsr2, during embryonic development. We demonstrate expression of both genes in a variety of organs and structures, such as kidney, eye, branchial arches and dermis. Both genes show a highly dynamic expression pattern with partially overlapping, but mostly distinct domains of expression. Special emphasis in this study was laid on the investigation of cOsr1 and cOsr2 in limb development, where we compared their expression pattern with the expression of Osr1 and Osr2 in the mouse.

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Section: Resultsmentioning

confidence: 99%

“…Both genes exhibit a dynamic expression pattern during mouse embryogenesis, showing predominant expression in intermediate mesoderm, branchial arches, kidneys and limbs (So and Danielian, 1999;Lan et al, 2001). Functionally, Osr1 and Osr2 have been characterised in the mouse by gene targeting.…”

Section: Resultsmentioning

confidence: 99%

Comparative expression pattern of Odd-skipped related genes Osr1 and Osr2 in chick embryonic development

Stricker

Brieske

Haupt

et al. 2006

Gene Expression Patterns

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“…Hey1 and Hes1 can also be grouped into the class C subfamily based on sequence comparison and can serve as transcriptional repressors or activators in a cell typedependent manner (21). OSR1 is a human homolog of murine odd-skipped related protein that plays an important role in embryonic development (22). NR2F2 is a nuclear receptor that is involved in the modulation of steroidogenic enzymes in human ovary (23), and SFRS10 is a splicing factor involved in the splicing of calcitonin (24).…”

Section: Resultsmentioning

confidence: 99%

Identification of p53 regulators by genome-wide functional analysis

Huang

Raya

DeJesus

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

142

128

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The p53 tumor-suppressor protein is a critical mediator of cellular growth arrest and the induction of apoptosis. To identify proteins involved in the modulation of p53 transcriptional activity, a gainof-function cellular screen was carried out with an arrayed matrix of Ϸ20,000 cDNAs. Nine genes previously unknown to be involved in regulating p53 activity were identified. Overexpression of seven of these genes (Hey1, Hes1, TFAP4, Osr1, NR2F2, SFRS10, and FLJ11339) resulted in up-regulation of p53 activity; overexpression of two genes (M17S2 and cathepsin B) resulted in down-regulation of p53 activity in mammalian cells. HES1, HEY1, and TFAP4, which are members of the basic helix-loop-helix transcription family, and OSR1 were shown to activate p53 through repression of HDM2 transcription. Ectopic expression of these basic helix-loop-helix transcription factors in both zebrafish and avian developmental systems activated p53 and induced apoptosis in vivo, resulting in a phenotype similar to that of p53 overexpression. Furthermore, ras-and myc-mediated transformation of mouse embryonic fibroblasts was abrogated by expression of HEY1 in a p53-dependent manner. These results suggest that these transcription factors are members of an evolutionarily conserved network that governs p53 function.

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“…Riboprobes were obtained or generated from the coding region of mouse Gdnf (Srinivas et al, 1999) (obtained from F. Costantini, Columbia University), Wt1 , Vegf164 (bases 75-669 in the murine cDNA, amplified by PCR), Pax2 [(Dressler et al, 1990), obtained from P. Grus], Nanog [generated in our laboratory cDNA 558-1140 (Chambers et al, 2003;Mitsui et al, 2003)], Osr1 [(So and Danielian, 1999), obtained from P. Danielian, Chester Beatty Laboratories, London] and Wnt4 [ (Stark et al, 1994), obtained from S. Vainio, University of Oulu, Finland], and subcloned in pCDNA3 or pCRII-TOPO (Invitrogen). Sense and antisense probes were synthesized and labeled with digoxigenin-UTP (Roche).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Angioblast-mesenchyme induction of early kidney development is mediated by Wt1 and Vegfa

et al. 2005

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Most studies on kidney development have considered the interaction of the metanephric mesenchyme and the ureteric bud to be the major inductive event that maintains tubular differentiation and branching morphogenesis. The mesenchyme produces Gdnf, which stimulates branching, and the ureteric bud stimulates continued growth of the mesenchyme and differentiation of nephrons from the induced mesenchyme. Null mutation of the Wt1 gene eliminates outgrowth of the ureteric bud, but Gdnf has been identified as a target of Pax2, but not of Wt1. Using a novel system for microinjecting and electroporating plasmid expression constructs into murine organ cultures, it has been demonstrated that Vegfa expression in the mesenchyme is regulated by Wt1. Previous studies had identified a population of Flk1-expressing cells in the periphery of the induced mesenchyme, and adjacent to the stalk of the ureteric bud, and that Vegfa was able to stimulate growth of kidneys in organ culture. Here it is demonstrated that signaling through Flk1 is required to maintain expression of Pax2 in the mesenchyme of the early kidney, and for Pax2 to stimulate expression of Gdnf. However, once Gdnf stimulates branching of the ureteric bud, the Flk1-dependent angioblast signal is no longer required to maintain branching morphogenesis and induction of nephrons. Thus,this work demonstrates the presence of a second set of inductive events,involving the mesenchymal and angioblast populations, whereby Wt1-stimulated expression of Vegfa elicits an as-yet-unidentified signal from the angioblasts, which is required to stimulate the expression of Pax2 and Gdnf,which in turn elicits an inductive signal from the ureteric bud.

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Cloning and expression analysis of a mouse gene related to Drosophila odd-skipped

Cited by 69 publications

References 14 publications

Comparative expression pattern of Odd-skipped related genes Osr1 and Osr2 in chick embryonic development

Comparative expression pattern of Odd-skipped related genes Osr1 and Osr2 in chick embryonic development

Identification of p53 regulators by genome-wide functional analysis

Angioblast-mesenchyme induction of early kidney development is mediated by Wt1 and Vegfa

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