Cloning and Expression Analysis of a Novel Mouse Zinc Finger Protein Gene Znf313 Abundantly Expressed in Testis

Li, Na; Sun, Hua Qin; Wu, Qia Qing; Tao, Da Chang; Zhang, Si Zhong; Xin, Yong

doi:10.5483/bmbrep.2007.40.2.270

Cited by 6 publications

(9 citation statements)

References 12 publications

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“…As HIT-4 and ZNF597 are derived from the same genome region, heterozygous gene deficiency of ZNF597 might also influence testis function, potentially spermatogenesis (Tascou et al 2001;Li et al 2007). However, pairing of male and female heterozygous mutants displayed no apparent problem in reproduction.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and gene disruption of a novel zinc‐finger protein, HIT‐4, expressed in rodent brain

Tanabe

Hirano

Iwasato

et al. 2010

Journal of Neurochemistry

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J. Neurochem. (2010) 112, 1035–1044. Abstract To identify a novel regulatory factor involved in brain development or synaptic plasticity, we applied the differential display PCR method to mRNA samples from NMDA‐stimulated and un‐stimulated neocortical cultures. Among 64 cDNA clones isolated, eight clones were novel genes and one of them encodes a novel zinc‐finger protein, HIT‐4, which is 317 amino acid residues (36–38 kDa) in length and contains seven C2H2 zinc‐finger motifs. Rat HIT‐4 cDNA exhibits strong homology to human ZNF597 (57% amino acid identity and 72% homology) and identity to rat ZNF597 at the carboxyl region. Furthermore, genomic alignment of HIT‐4 cDNA indicates that the alternative use of distinct promoters and exons produces HIT‐4 and ZNF597 mRNAs. Northern blotting revealed that HIT‐4 mRNA (∼6 kb) is expressed in various tissues such as the lung, heart, and liver, but enriched in the brain, while ZNF597 mRNA (∼1.5 kb) is found only in the testis. To evaluate biological roles of HIT‐4/ZNF597, targeted mutagenesis of this gene was performed in mice. Homozygous (−/−) mutation was embryonic lethal, ceasing embryonic organization before cardiogenesis at embryonic day 7.5. Heterozygous (+/−) mice were able to survive but showing cell degeneration and vacuolization of the striatum, cingulate cortex, and their surrounding white matter. These results reveal novel biological and pathological roles of HIT‐4 in brain development and/or maintenance.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization and gene disruption of a novel zinc‐finger protein, HIT‐4, expressed in rodent brain

Tanabe

Hirano

Iwasato

et al. 2010

Journal of Neurochemistry

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“…The ZNF313 gene is expressed in a wide range of tissues including skin, heart, lung, liver and kidney, etc., with the highest transcript levels found in testis (Capon et al, 2008). The transcription of human ZNF313 in the testes of azoospermic fetus is much less than in the testes of a fertile adult, suggesting that the expression of ZNF313 may be implicated in the formation of spermatids (Ma et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…While C2H2, C3HC4 and C2HC are the most extensive and common types of the zinc‐binding motif, all of them exist in the ZNF313 protein. The ZNF313 gene (also known as RNF114) consists of six exons and encodes a 228‐amino acid protein with a C3HC4 ring finger, a C2HC domain and two C2H2 domains, mapping to chromosome 20q13 (Ma et al, 2003; Giannini et al, 2008). The ZNF313 gene is expressed ubiquitously with the highest in testis, but the transcription level is much less in testes of azoospermic patient and fetus (Ma et al, 2003; Capon et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The ZNF313 gene (also known as RNF114) consists of six exons and encodes a 228‐amino acid protein with a C3HC4 ring finger, a C2HC domain and two C2H2 domains, mapping to chromosome 20q13 (Ma et al, 2003; Giannini et al, 2008). The ZNF313 gene is expressed ubiquitously with the highest in testis, but the transcription level is much less in testes of azoospermic patient and fetus (Ma et al, 2003; Capon et al, 2008). The mouse Znf313 is also expressed in many tissues even in early embryo sharing more than 89% identity with the human ZNF313 gene (Li et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…The ZNF313 gene is expressed ubiquitously with the highest in testis, but the transcription level is much less in testes of azoospermic patient and fetus (Ma et al, 2003;Capon et al, 2008). The mouse Znf313 is also expressed in many tissues even in early embryo sharing more than 89% identity with the human ZNF313 gene (Li et al, 2007). These findings suggest that the ZNF313 gene might play a fundamental role in testis-specific transcription during spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Sp1 plays an important role in regulating the transcription of ZNF313

Liao

Deng

et al. 2010

Cell Biology International

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The ZNF313 gene has the highest transcription level in fertile male testes and may be related to human spermatogenesis. The deletion-mutated plasmids of ZNF313 promoter were constructed and transfected into HEK293 cells. The result showed that the fragment from nt -157 to +8 has a basal transcriptional activity. A functional Sp1 binding site was identified by site-directed mutation test and mithramycin A treatment. A 447-bp based at +233 to -213 exhibits a characteristic CpG island, which overlaps with the promoter region. Our work suggests that ZNF313 is controlled at the transcriptional level, and a common mechanism controlling the basal transcription of ZNF313 gene exists.

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Identification of RNF114 as a novel positive regulatory protein for T cell activation

Yang

et al. 2014

Immunobiology

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Cloning and Expression Analysis of a Novel Mouse Zinc Finger Protein Gene Znf313 Abundantly Expressed in Testis

Cited by 6 publications

References 12 publications

Molecular characterization and gene disruption of a novel zinc‐finger protein, HIT‐4, expressed in rodent brain

Molecular characterization and gene disruption of a novel zinc‐finger protein, HIT‐4, expressed in rodent brain

Sp1 plays an important role in regulating the transcription of ZNF313

Identification of RNF114 as a novel positive regulatory protein for T cell activation

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