Cloning and Expression of Putative Rac/Rop GTPase Genes, Am-rac1 and Am-rac2, Involved in Methyl Jasmonate-Induced Transcriptional Activation of Farnesyl Diphosphate Synthase in Cell Cultures of Aquilaria microcarpa

Kenmotsu, Yoshihide; Asano, K.; Yamamura, Yoshimi; Kurosaki, Fumiya

doi:10.1007/s11105-012-0529-0

Cited by 10 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, MJ-induced transcriptional activation of δ-GS was appreciably inhibited in the presence of NSC23766, and this set of the results appeared to be reproducible in repeated experiments. These observations suggest that calmodulin-and Rac GTPase-dependent cellular processes are involved in transcriptional activation of δ-GS in A. microcarpa cells triggered by MJ, and are consistent with our previous findings that Rac GTPase would play roles in the activation of Ca 2+ -cascade in MJ-signaling processes of higher plant cells (Kasidimoko et al, 2005;Kenmotsu et al, 2010;Kenmotsu et al, 2013).…”

Section: Contents Lists Available At Sciencedirectsupporting

confidence: 92%

“…microcarpa cell cultures (Kenmotsu et al, 2013), and found that rac2 translate might function in MJ-induced elevation of biosynthetic activity of sesquiterpene compounds. In addition, we generated two rac2 mutants: a constitutively active (CA) and a dominant negative (DN) forms deemed CArac2 and DNrac2, respectively.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 98%

“…It has been also shown that calmodulin, a Ca 2+ -binding protein, functions as a key mediator in the signal transduction mechanisms of MJ. In addition, we have recently demonstrated (Saitoh et al, 2007;Shite et al, 2009;Mitamura et al, 2011;Kenmotsu et al, 2013) that Rac GTPase, a plant-specific monomeric GTP-binding protein (Zeng and Yang, 2000;Gu et al, 2004;Berken, 2006), would play important roles to evoke Ca 2+ -influx in MJ-signaling processes of higher plant cells ( Fig. 1).…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…Murashige and Skoog (1962) agar medium supplemented with 3 μM of 2,4-dichlorophenoxyacetic acid and 3 μM of N 6 -benzyladenine at 26°C under darkness (Kenmotsu et al, 2010), and the expression levels of δ-GS were measured semiquantitatively using RT-PCR. A. microcarpa cells (3-week-old, approximately 100 mg) were pre-incubated on the medium containing N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) (BIOMOL Research Laboratory, 100 or 25 μM) or NSC23766 (Calbiochem, final concentration 100 or 25 μM) for 4 days according to the method described previously (Kenmotsu et al, 2013). MJ (100 μM in 100-μl aliquots) was then added to the cultures, and the cells were further incubated for 6 h. Then, total RNA was isolated from cultured cells using the RNeasy Plant Mini Kit (Qiagen), and cDNA templates were generated by RT reaction.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptional activation of sesquiterpene biosynthetic enzyme δ-guaiene synthase gene in cell cultures of Aquilaria microcarpa overexpressing cam1 and rac2 encoding calmodulin and Rac GTPase

Kurosaki

Taura

2015

Plant Gene

View full text Add to dashboard Cite

a b s t r a c tExpression level of a sesquiterpene biosynthetic enzyme gene, δ-GS, encoding δ-guaiene synthase of Aquilaria microcarpa markedly elevated when cultured cells of the plant were treated with methyl jasmonate. The methyl jasmonate-induced expression of δ-GS was, however, appreciably reduced in a dose-dependent manner in the presence of N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide and NSC23766, the specific inhibitors for calmodulin-and Rac GTPase-dependent cellular processes. Transformation of A. microcarpa cell culture by cam1 and rac2, calmodulin and Rac GTPase genes isolated from the plant, triggered the transcriptional activation of δ-GS even in the absence of methyl jasmonate. In contrast, A. microcarpa cultures overexpressing mutated rac2 of which translate is lacking in binding ability toward GTP did not show the significant expression of δ-GS gene. These results suggest that calmodulin and Rac GTPase proteins function as the important mediators in jasmonate-induced activation of sesquiterpene biosynthesis in A. microcarpa.

show abstract

Section: Contents Lists Available At Sciencedirectsupporting

confidence: 92%

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 98%

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptional activation of sesquiterpene biosynthetic enzyme δ-guaiene synthase gene in cell cultures of Aquilaria microcarpa overexpressing cam1 and rac2 encoding calmodulin and Rac GTPase

Kurosaki

Taura

2015

Plant Gene

View full text Add to dashboard Cite

show abstract

“…To study the signal transduction of the MJ-induced sesquiterpene biosynthesis, two cDNA clones presumably encoding calmodulin (CAM), a Ca 2+ -binding protein, and two cDNA clones presumably encoding Rac/Rop GTPases, a plant-specifi c monomeric GTP-binding protein, were isolated based on the reported amino acids sequences from A. microcarpa calli (Kenmotsu et al 2010(Kenmotsu et al , 2013. These clones were designated Am -cam -1 , Am -cam -2 , Am -rac1 , and Am -rac2 , respectively.…”

Section: Identifi Cation Of the Regulator Genes In A Microcarpamentioning

confidence: 99%

Molecular Mechanism Studies of Terpenoid Biosynthesis in Agarwood

Gao

Wei

2016

Tropical Forestry

View full text Add to dashboard Cite

The wound-induced natural production of agarwood hindered the development of its industry. Effi cient and sustainable manner for its production should be developed. Metabolic engineering is one of the techniques with incredible potential to solve this problem. However, its realization should depend on the deep understanding of the whole biosynthetic pathway of the agarwood constituents. Here, we introduce studies of the molecular mechanism of the main agarwood constituentsterpenoids -to have an overview of the development in the study of the terpene biosynthesis pathway and the possible regulators of terpene biosynthesis in agarwood, including miRNAs and the members in the signal transduction. We also discuss the efforts that should be undertaken in the future.

show abstract