Cloning and expression of two sterol C-24 methyltransferase genes from upland cotton (Gossypium hirsuturm L.)

Luo, Ming; Tan, Kunling; Zhong-yi, Xiao; Hu, Mingyu; Liao, Peng; Chen, Kuijun

doi:10.1016/s1673-8527(08)60052-1

Cited by 20 publications

(9 citation statements)

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“…We observed a high concentration of sitosterol, a major phytosterol in cotton fiber, which was consistent with the observations of previous reports (Gou et al, 2007;Wanjiea et al, 2005). The biosynthetic genes of phytosterols, such as GhSMT2-1, GhSMT2-2, GhSMT1, GhCYP51G1, and GhHYDRA1, are preferentially expressed in the rapidly elongating fibers (Gou et al, 2007;Liu et al, 2012;Luo et al, 2008;Padmalatha et al, 2012;Shi et al, 2006;Tan et al, 2009;Wanjiea et al, 2005;Zang et al, 2011), indicating that phytosterols was essential for fiber elongation. Inhibition of phytosterol biosynthesis by tridemorph suppressed fiber elongation in vivo or in planta.…”

Section: Role Of Phytosterols In the Elongation Of Cotton Fiberssupporting

confidence: 92%

“…Their homologues in upland cotton (Gossypium hirsutum L.) have been cloned in recent years (Luo et al, 2007(Luo et al, , 2008Shi et al, 2006;Tan et al, 2009;Zang et al, 2011). We compared the level of expression of twelve genes associated with phytosterol biosynthesis between the fibers and ovules of the li-1 mutant and TM-1.…”

Section: Tridemorph Suppresses the Elongation Of Fiber Cellsmentioning

confidence: 99%

“…A large number of phytosterols, important constituents of the cell membrane, must be synthesized to meet the needs of the dramatic increase in cell size during rapid elongation. Previous studies have shown that genes involved in phytosterol biosynthesis, such as GhSMT2-1 (sterol methyltransferase 2), GhSMT2-2, GhSMT1 (sterol methyltransferase 1), GhCYP-51G1 (obtusifoliol 14-demethylase), and GhHYDRA1 (∆ 8 -∆ 7 -sterol isomerase), are preferentially expressed in rapidly elongating fibers (Gou et al, 2007;Liu et al, 2012;Luo et al, 2008;Padmalatha et al, 2012;Shi et al, 2006;Tan et al, 2009;Wanjiea et al, 2005;Zang et al, 2011). Furthermore, BRs have been reported to play an important role in fiber growth (Luo et al, 2007;Kasukabe et al, 2001;Shi et al, 2006;Sun et al, 2004.…”

Section: Introductionmentioning

confidence: 99%

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Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation

Deng

Wei

Tan

et al. 2016

Sci. China Life Sci.

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Phytosterols play an important role in plant growth and development, including cell division, cell elongation, embryogenesis, cellulose biosynthesis, and cell wall formation. Cotton fiber, which undergoes synchronous cell elongation and a large amount of cellulose synthesis, is an ideal model for the study of plant cell elongation and cell wall biogenesis. The role of phytosterols in fiber growth was investigated by treating the fibers with tridemorph, a sterol biosynthetic inhibitor. The inhibition of phytosterol biosynthesis resulted in an apparent suppression of fiber elongation in vitro or in planta. The determination of phytosterol quantity indicated that sitosterol and campesterol were the major phytosterols in cotton fibers; moreover, higher concentrations of these phytosterols were observed during the period of rapid elongation of fibers. Furthermore, the decrease and increase in campesterol : sitosterol ratio was associated with the increase and decease in speed of elongation, respectively, during the elongation stage. The increase in the ratio was associated with the transition from cell elongation to secondary cell wall synthesis. In addition, a number of phytosterol biosynthetic genes were down-regulated in the short fibers of ligon lintless-1 mutant, compared to its near-isogenic wild-type TM-1. These results demonstrated that phytosterols play a crucial role in cotton fiber development, and particularly in fiber elongation. cotton fiber, phytosterols, gene expression, tridemorph, ligon lintless-1 Citation:Deng, S., Wei, T., Tan, K., Hu, M., Li, F., Zhai, Y., Ye, S., Xiao, Y., Hou, L., Pei, Y., and Luo, M. (2016). Phytosterol content and the campesterol : sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation. Sci China Life Sci 59, 183 -193.

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Section: Role Of Phytosterols In the Elongation Of Cotton Fiberssupporting

confidence: 92%

Section: Tridemorph Suppresses the Elongation Of Fiber Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation

Deng

Wei

Tan

et al. 2016

Sci. China Life Sci.

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“…In cucumber, the inhibition of BR biosynthesis by Brz resulted in higher expression levels of BR6ox1 and SMT , two genes involved in BR biosynthesis, while EBR treated, pollinated and natural parthenocarpic fruits all had reduced expression of these genes (Fu et al ). The transcriptional regulation of plant SMT genes of the sterol pathway has been investigated in different plant species (Shi et al , Diener et al , Luo et al , Neelakandan et al , ). Overexpression of SMT2 reportedly lowered the level of 24‐methylsterols (campesterol) and concomitantly raised the level of 24‐ethylsterols (β‐sitosterol), as well as a diminishing the BR content and the growth rates (Schaeffer et al , Sitbon and Jonsson , Boutté and Grebe ).…”

Section: Resultsmentioning

confidence: 99%

Regulation of sterol content and biosynthetic gene expression during flower opening and early fruit development in olive

Inês

Corbacho

Paredes

et al. 2019

Physiologia Plantarum

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Phytosterols are lipophilic membrane components essential not only for diverse cellular functions but also are biosynthetic precursors of the plant hormone, brassinosteroid (BR). However, the interaction between phytosterol and BR during early fleshy-fruit growth remains largely uncharacterized. In olive, phytosterols are important lipids because they affect oil quality, but phytosterol composition during flowering and early fruit development has not been explored. Here, we first investigated the temporal changes in phytosterol composition, and biosynthetic gene expression that occurred during olive flower opening and early fruit growth. Next, we analyzed the interrelationship between phytosterol and BR, whose levels we manipulated through the application of exogenous BRs (24-epibrassinolide, EBR) or a BR biosynthesis inhibitor (brassinazole, Brz). In this report, the profiling of phytosterol measurement revealed that -sitosterol is the most abundant in olive reproductive organs. Our data demonstrate that both OeCYP51 and OeSMT2 genes are upregulated during floral anthesis in good agreement with the rise in cholesterol and -sitosterol contents in olive flower. By contrast, the OeCYP51 and OeSMT2 genes displayed different expression patterns during early olive-fruit development. Furthermore, our data show that exogenous EBR enhanced the early olive-fruit growth, as well as the OeSMT2 transcript and -sitosterol levels, but decreased the OeCYP51 transcript, squalene, campesterol and cholesterol levels, whereas the Brz treatment exerted the opposite effect. Overall, our findings indicate an up-regulation of -sitosterol biosynthesis by BR at the transcriptional level during early olive-fruit growth, providing a valuable tool to unravel the physiological function of SMT2 in future studies.

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“…This step represents a branch point reaction directing carbon flux towards synthesis of C-24 ethyl sterols and away from brassinosteroid biosynthesis. The transcriptional regulation of plant SMT1 and SMT2 genes of the sterol pathway have been investigated in different plant species (Carland et al 2002;Diener et al 2000;Luo et al 2008;Neelakandan et al 2009;Shi et al 1996). The mutants of the SMT2-1 gene in Arabidopsis, cotyledon vascular pattern 1 (cvp1), has pleiotropic phenotypes including a defect in vascular patterning and development, serrated petal and sepal margins and reduced intermodal and silique elongation (Carland et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and functional analysis of Glycine max sterol methyl transferase 2 genes involved in plant membrane sterol biosynthesis

et al. 2010

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Sterol C24 methyltransferase (SMT2) genes governing the pattern of phytosterols synthesized in higher plants have been studied in Glycine seedlings and wild-type and engineered Arabidopsis thaliana plants. The SMT2 genes of soybean (SMT2-1 and SMT2-2) previously cloned and characterized (Neelakandan et al. 2009) were shown to complement the SMT deficient cvp1 mutant Arabidopsis plants, consistent with their role in regulation of 24-alkyl sterol-controlled plant physiology. Further analysis of these genes showed that environmental cues, including dehydration, cold, and abscisic acid induced differential changes in transcript levels of the SMT2 during soybean seedling growth. Sterol analyses of transgenic Arabidopsis seeds originating in variant constructs of AtHMGR1, GmSMT1, and GmSMT2 engineered in seeds showed relevant modifications in the ratio of 24-methyl to 24-ethyl sterol in the direction of sitosterol formation. To provide insight into the structural features of the sterol gene that affects transcript regulation, the upstream promoter sequences of soybean SMT2 genes were cloned and characterized. Sequence analysis revealed several important cis-elements and transcription factor binding sites. The analysis of promoter-GUS fusions in transgenic Arabidopsis plants revealed shared and distinct expression features in different developmental stages and tissues. The data are interpreted to imply that SMT2 is an important contributor to normal plant growth and development.

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Cloning and expression of two sterol C-24 methyltransferase genes from upland cotton (Gossypium hirsuturm L.)

Cited by 20 publications

References 27 publications

Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation

Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation

Regulation of sterol content and biosynthetic gene expression during flower opening and early fruit development in olive

Molecular characterization and functional analysis of Glycine max sterol methyl transferase 2 genes involved in plant membrane sterol biosynthesis

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