Cloning and expression of two proopiomelanocortin mRNAs in the common carp (Cyprinus carpio L.)

Arends, R.J.; Vermeer, Hendrika; Martens, Gerard J.M.; Leunissen, J.A.M.; Bonga, S.E. Wendelaar; Flik, G.

doi:10.1016/s0303-7207(98)00139-7

Cited by 77 publications

(46 citation statements)

References 40 publications

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“…In carp, the two POMC molecules produce the same type of -MSH and -endorphin peptides, but two different types of ACTH and -MSH (Arends et al 1998). As the rapid drop in temperature described here induces an -MSHindependent rise in cortisol (S E Wendelaar Bonga, unpublished observations), we hypothesize that the corticotropic cells are involved in this response.…”

Section: Figurementioning

confidence: 56%

“…In the present study RT-PCR combined with CE-LIF allowed us to quantify differences in expression of closely related POMC genes (93·5% nucleotide sequence identity (Arends et al 1998) from a tetraploid carp. The fact that no digestion of POMC-I by PvuII and no digestion of POMC-II by SacI occurred indicates the specificity of the enzymes used.…”

Section: Discussionmentioning

confidence: 99%

“…In the common carp, the duplicate loci started to diverge from each other only some 16 million years ago (Larhammar & Risinger 1993). For this carp two POMC cDNAs (POMC-I and POMC-II) have been cloned; as the two POMC molecules show substitutions in their -MSH and corticotropin-like intermediate peptide (CLIP) regions, different POMC end products with specific functions may be expected (Arends et al 1998). The expression of two POMCs in tetraploid animals has been known for many years (Kawauchi 1983, Martens et al 1985, Salbert et al 1992), but there is as yet little evidence for the biological significance of the presence of two gene transcripts.…”

Section: Introductionmentioning

confidence: 99%

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Differential expression of two pro-opiomelanocortin mRNAs during temperature stress in common carp (Cyprinus carpio L.)

Arends¹,

Gaag²,

Martens³

et al. 1998

Journal of Endocrinology

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Pro-opiomelanocortin (POMC) is the precursor of a number of biologically active peptides, including adrenocorticotropic hormone, -melanocyte-stimulating hormone and -endorphin, which are released by the pituitary glands of fish as well as mammals. To quantify the levels of expression of the two POMC mRNAs relative to one another during the response of the common carp to temperature-induced stress, we used reverse transcriptase PCR combined with capillary electrophoresis and laserinduced fluorescence detection. The ratio of POMC-I mRNA to POMC-II mRNA determined in wild-type and four isogenic carp strains was found to be straindependent and influenced by temperature. In strain E20 R8, the ratio had altered in favour of POMC-I from 1:3·2 (POMC-I:POMC-II) in fish adapted to 24 C to 1:1·2 in fish adapted to a decrease of 9 C in ambient temperature. A rapid drop in temperature from 24 to 15 C decreased the POMC mRNA ratio at the expense of POMC-I from 1:1·9 in the control fish (strain E4 R3R8) to 1:4·2 3 h after the temperature drop of 9 C. We conclude that both POMC genes are expressed in the common carp and that their expression ratio is strain-dependent and changes in response to ambient temperature.

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Section: Figurementioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential expression of two pro-opiomelanocortin mRNAs during temperature stress in common carp (Cyprinus carpio L.)

Arends¹,

Gaag²,

Martens³

et al. 1998

Journal of Endocrinology

Self Cite

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“…POMC, the precursor of melanocortin, is mainly processed to alpha-melanocyte-stimulating hormone (a-MSH) and b-endorphin (Cerda-Reverter and Agulleiro, 2011). POMC I and POMC II have already been identified in common carp (Arends et al, 1998). The melanocortin system has been illustrated to play a critical role in the regulation of food intake and body weight in fish (Cerda-Reverter and Agulleiro, 2011).…”

Section: Introductionmentioning

confidence: 99%

Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)

Zhong

Song

Wang

et al. 2013

General and Comparative Endocrinology

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“…The subfamily shares similar peptide sequences with opioid precursors, but also contains other non-opioid peptides such as ACTH and alpha-MSH, which are involved in the stress response and stimulate corticosteroid release (Arends et al, 1998).…”

Section: The Subfamily Of Pomc-derived Peptidesmentioning

confidence: 99%

A Pattern Search Method for Discovering Conserved Motifs in Bioactive Peptide Families

Liu¹,

Schoofs²,

Baggerman³

et al. 2011

Bioinformatics - Trends and Methodologies

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Cloning and expression of two proopiomelanocortin mRNAs in the common carp (Cyprinus carpio L.)

Cited by 77 publications

References 40 publications

Differential expression of two pro-opiomelanocortin mRNAs during temperature stress in common carp (Cyprinus carpio L.)

Differential expression of two pro-opiomelanocortin mRNAs during temperature stress in common carp (Cyprinus carpio L.)

Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)

A Pattern Search Method for Discovering Conserved Motifs in Bioactive Peptide Families

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