Cloning and expression of two hepcidin genes in the mudskipper ( Boleophthalmus pectinirostris ) provides insights into their roles in male reproductive immunity

Li, Zhe; Wang, Hong; Qiu, Hengtong; Zhang, Yu Ting; Yang, Moon-Sik; You, Xin; Chen, Shi Xi

doi:10.1016/j.fsi.2016.07.025

Cited by 27 publications

(14 citation statements)

References 35 publications

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“…In the mammalian and avian reproductive tissues, several types of AMPs have been described, as in fish [75][76][77][78][79]. Thus, as described previously, two hepcidins have been detected in the testis of mudskipper, one of them (hepcidin-2) being regulated by gonadotrophins and stimulated by LPS [56]. In addition, a β-defensin in orange-spotted grouper is specifically expressed in pituitary and testis, though it shows a steady expression in the pituitary its expression in the gonad increased during sex reversal from female to male.…”

Section: The Gonad Immune Responses In European Sea Bass-preliminary mentioning

confidence: 55%

“…Regarding gonad-specific AMPs, in the testis of mudskipper (Boleophthalmus pectinirostris) two hepcidins have been described. Interestingly, hepcidin-2 is highly produced in Leydig cells, regulated by gonadotrophins, and up-regulated upon lipopolysaccharide (LPS) stimulation [56]. In the orange-spotted grouper (Epinephelus coioides), a β-defensin specifically expressed in pituitary and testis has also been cloned [57].…”

Section: Immune Effectors Out Of the Blood-testis Barriermentioning

confidence: 99%

“…For example, it has been suggested that the reproductive tract of mudskipper synthesizes specific antimicrobial compounds to clean fertilized eggs before hatching and that Hepcidin-1 and The production of specific AMPs has been related with several reproductive issues. For example, it has been suggested that the reproductive tract of mudskipper synthesizes specific antimicrobial compounds to clean fertilized eggs before hatching and that Hepcidin-1 and Hepcidin-2 play a key role in pathogen clearance during the reproductive process [56]. In hermaphroditic orange spotted grouper, beta-defensin isolated from pituitary is mainly expressed in testis [57].…”

Section: Description Of Antimicrobial Peptidesmentioning

confidence: 99%

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Immune-Endocrine Interactions in the Fish Gonad during Infection: An Open Door to Vertical Transmission

Valero

Cuesta

Cammarata

et al. 2018

Fishes

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Abstract:The interaction between the immune and endocrine systems has long been recognized in vertebrates. In fish, it is known that the prevalence and intensity of such infections are higher in males than in females and probably related to sex steroid hormone levels. In addition, the immune response in the fish gonad tissues is specifically regulated to prevent infertility. This condition is used by some pathogens to colonize the fish gonad, evade the systemic immune response, and so spread to the progeny. This review brings up to date our knowledge concerning fish gonad immunity and its regulation, immune-endocrine interactions, and how some pathogens use this tissue to spread to the progeny through vertical transmission. More specifically, we will look at the case of the European sea bass and nodavirus (NNV). Sea bass is a very susceptible fish species to NNV infections, and this virus has been associated to vertical transmission since it is detected in gonad fluids as well as in testicular gametes. In fact, sea bass immunity in the gonad is regulated in a very different way to other target tissues (brain and retina) or immune-relevant tissues (head-kidney or spleen).

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Section: The Gonad Immune Responses In European Sea Bass-preliminary mentioning

confidence: 55%

Section: Immune Effectors Out Of the Blood-testis Barriermentioning

confidence: 99%

Section: Description Of Antimicrobial Peptidesmentioning

confidence: 99%

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Immune-Endocrine Interactions in the Fish Gonad during Infection: An Open Door to Vertical Transmission

Valero

Cuesta

Cammarata

et al. 2018

Fishes

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“…Under physiological conditions, these isoforms are found at low concentrations in urine and plasma, which increase during pathological processes such as sepsis. The isoform composed of 20 amino acids is related to the elevated levels of plasma creatinine, but the underlying mechanism has not been elucidated [21,22]. …”

Section: Hepcidinmentioning

confidence: 99%

Hepcidin: Homeostasis and Diseases Related to Iron Metabolism

et al. 2017

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Iron is an essential metal for cell survival that is regulated by the peptide hormone hepcidin. However, its influence on certain diseases is directly related to iron metabolism or secondary to underlying diseases. Genetic alterations influence the serum hepcidin concentration, which can lead to an iron overload in tissues, as observed in haemochromatosis, in which serum hepcidin or defective hepcidin synthesis is observed. Another genetic imbalance of iron is iron-refractory anaemia, in which serum concentrations of hepcidin are increased, precluding the flow and efflux of extra- and intracellular iron. During the pathogenesis of certain diseases, the resulting oxidative stress, as well as the increase in inflammatory cytokines, influences the transcription of the HAMP gene to generate a secondary anaemia due to the increase in the serum concentration of hepcidin. To date, there is no available drug to inhibit or enhance hepcidin transcription, mostly due to the cytotoxicity described in the in vitro models. The proposed therapeutic targets are still in the early stages of clinical trials. Some candidates are promising, such as heparin derivatives and minihepcidins. This review describes the main pathways of systemic and genetic regulation of hepcidin, as well as its influence on the disorders related to iron metabolism.

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“…Our finding on the strong expression of LEAP-2A in the mud loach ovary was similar with the observation from the grass carp Ctenopharyngodon idella (Liu et al 2010), however, apparently different from findings in common carp C. carpio (Yang et al 2014) and blunt snout bream Megalobrama amblycephala (Liang et al 2013) to represent very low or negligible expression of LEAP-2 in gonadic tissues. Meanwhile, the protective role of a hepcidin (LEAP-1) isoform in male reproductive immunity has been reported in the mudskipper Boleophthalmus pectinirostris (Li et al 2016).…”

Section: Expression Pattern In Adult Tissuesmentioning

confidence: 99%

Gene structure and expression characteristics of liver-expressed antimicrobial peptide-2 isoforms in mud loach (Misgurnus mizolepis, Cypriniformes)

Lee

Nam

2017

Fish Aquatic Sci

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Background: Liver-expressed antimicrobial peptide-2 (LEAP-2) is an important component of innate immune system in teleosts. In order to understand isoform-specific involvement and regulation of LEAP-2 genes in mud loach (Misgurnus mizolepis, Cypriniformes), a commercially important food fish, this study was aimed to characterize gene structure and expression characteristics of two paralog LEAP-2 isoforms. Results: Mud loach LEAP-2 isoforms (LEAP-2A and LEAP-2B) showed conserved features in the core structure of mature peptides characterized by four Cys residues to form two disulfide bonds. The two paralog isoforms represented a tripartite genomic organization, known as a common structure of vertebrate LEAP-2 genes. Bioinformatic analysis predicted various transcription factor binding motifs in the 5′-flanking regions of mud loach LEAP-2 genes with regard to development and immune response. Mud loach LEAP-2A and LEAP-2B isoforms exhibited different tissue expression patterns and were developmentally regulated. Both isoforms are rapidly modulated toward upregulation during bacterial challenge in an isoform and/or tissue-dependent fashion.

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Cloning and expression of two hepcidin genes in the mudskipper ( Boleophthalmus pectinirostris ) provides insights into their roles in male reproductive immunity

Cited by 27 publications

References 35 publications

Immune-Endocrine Interactions in the Fish Gonad during Infection: An Open Door to Vertical Transmission

Immune-Endocrine Interactions in the Fish Gonad during Infection: An Open Door to Vertical Transmission

Hepcidin: Homeostasis and Diseases Related to Iron Metabolism

Gene structure and expression characteristics of liver-expressed antimicrobial peptide-2 isoforms in mud loach (Misgurnus mizolepis, Cypriniformes)

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