Cloning and expression profiling of odorant‐binding proteins in the tarnished plant bug, Lygus lineolaris

Abstract

In insects, the perception and discrimination of odorants requires the involvement of odorant-binding proteins (OBPs). To gain a better molecular understanding of olfaction in the agronomic pest Lygus lineolaris (the tarnished plant bug), we used a transcriptomics-based approach to identify potential OBPs. In total, 33 putative OBP transcripts, including the previously reported Lygus antennal protein (LAP), were identified based on the characteristic OBP Cys signature and/or sequence similarity with annotated orthologous sequences. The L. lineolaris OBP (LylinOBP) repertoire consists of 20 'classic' OBPs, defined by the spacing of six conserved Cys residues, and 12 'Plus-C' OBPs, defined by the spacing of eight conserved Cys and one conserved Pro residue. Alternative splicing of OBP genes appears to contribute significantly to the multiplicity of LylinOBP sequences. Microarray-based analysis of chemosensory tissues (antennae, legs and proboscis) revealed enrichment of 21 LylinOBP transcripts in antennae, 12 in legs, and 15 in proboscis, suggesting potential roles in olfaction and gustation respectively. PCR-based determination of transcript abundance for a subset of the LylinOBP genes across multiple adult tissues yielded results consistent with the hybridization data.

Functional Characterization of Odorant Binding Protein 27 (RproOBP27) From Rhodnius prolixus Antennae

“…Thus, we cannot rule out the possibility that RproOBP26 might be involved in the transport of non-sensorial molecules in the gut, as well as semiochemicals in antennae. Although, only one gene for RproOBP26 has been annotated in the genome (Mesquita et al, 2015), we could not exclude the possibility of RproOBP26 has alternative splicing, as previously observed for other insect species (Forêt and Maleszka, 2006; Hull et al, 2014). …”

Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes inAthetis lepigone(Lepidoptera: Noctuidae)

“…OBP and CSP gene expression in the proboscis has been observed in other insects including Apolygus lucorum (Hua et al, 2012), Lygus lineolaris (Hull, Perera & Snodgrass, 2014), S. podoptera (Liu et al, 2015c), and A. dissimilis (Sun et al, 2016). Further functional studies have also confirmed the gustation function of some genes: OBP49a in D. melanogaster is involved in the suppression of sweet taste by bitter chemicals (Jeong et al, 2013); some OBPs in D. melanogaster can modulate sucrose intake in response to a panel of nine bitter compounds by RNAi-mediated methods (Swarup et al, 2014); and CSP4, which is exclusively presented in the proboscis of two sibling species— H. armigera and H. assulta —an act as a wetting agent to reduce the surface tension of aqueous solutions (Liu et al, 2014).…”

Identification and Expression Analysis of Candidate Odorant-Binding Protein and Chemosensory Protein Genes by Antennal Transcriptome of Sitobion avenae

“…lugens [22], and 21 of 33 OBPs in L . lineolaris [19]. Seven of 13 SaveOBPs ( SaveOBP6 , SaveOBP7 , SaveOBP9 , SaveOBP10 , SaveOBP13 , SaveOBP14 and SaveOBP15 ) were uniquely or primarily expressed in antennae compared with other tissues, indicating a vital olfactory role for these genes.…”

“…Genome sequencing facilitated the identification of 15 OBP genes in the hemipteran Acyrthosiphon pisum [18]. In contrast, mRNA-based transcriptome sequencing has led to the discovery of numerous OBPs from diverse hemipteran pests including Lygus lineolaris [19], Adelphocoris lineolatus [20], Aphis gossypii [21], Nilaparvata lugens [22, 23], Sogatella furcifera [24], and Apolygus lucorum [25]. Interestingly, the number of OBPs reported has differed depending on the species (eg.…”