Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize

Jin, Bo; Sheng, Zunlai; Ishfaq, Muhammad; Yang, Hongliang

doi:10.1371/journal.pone.0211941

Cited by 16 publications

(8 citation statements)

References 33 publications

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“…Cis -regulatory elements present in gene promoters are essential for understanding the regulatory mechanism of gene expression [ 22 , 23 ]. Jin et al [ 24 ] cloned the Zmap promoter from Zea mays and characterized several cis -regulatory elements present in its promoter, such as the MeJA-responsive element, drought-responsive element, and low-temperature element. Subsequently, β-glucuronidase (GUS) fluorometric assays confirmed the hypothesis that these elements strongly influence the expression of Zmap under MeJA, drought, and low-temperature conditions.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Functional Characterization of the Promoters of Miltiradiene Synthase Genes, TwTPS27a and TwTPS27b, and Interaction Analysis with the Transcription Factor TwTGA1 from Tripterygium wilfordii

Huo

Zhang

Chen

et al. 2021

Plants

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Miltiradiene synthase (MS) genes, TwTPS27a and TwTPS27b, are the key diterpene synthase genes in the biosynthesis of triptolide, which is an important medicinally active diterpenoid in Tripterygium wilfordii. However, the mechanism underlying the regulation of key genes TwTPS27a/b in triptolide biosynthesis remains unclear. In this study, the promoters of TwTPS27a (1496 bp) and TwTPS27b (1862 bp) were isolated and analyzed. Some hormone-/stress-responsive elements and transcription factor (TF) binding sites were predicted in both promoters, which might be responsible for the regulation mechanism of TwTPS27a/b. The β-glucuronidase (GUS) activity analysis in promoter deletion assays under normal and methyl jasmonate (MeJA) conditions showed that the sequence of −921 to −391 bp is the potential core region of the TwTPS27b promoter. And the TGACG-motif, a MeJA-responsive element found in this core region, might be responsible for MeJA-mediated stress induction of GUS activity. Moreover, the TGACG-motif is also known as the TGA TF-binding site. Yeast one-hybrid and GUS transactivation assays confirmed the interaction between the TwTPS27a/b promoters and the TwTGA1 TF (a MeJA-inducible TGA TF upregulating triptolide biosynthesis in T. wilfordii), indicating that TwTPS27a/b are two target genes regulated by TwTGA1. In conclusion, our results provide important information for elucidating the regulatory mechanism of MS genes, TwTPS27a and TwTPS27b, as two target genes of TwTGA1, in jasmonic acid (JA)-inducible triptolide biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Isolation and Functional Characterization of the Promoters of Miltiradiene Synthase Genes, TwTPS27a and TwTPS27b, and Interaction Analysis with the Transcription Factor TwTGA1 from Tripterygium wilfordii

Huo

Zhang

Chen

et al. 2021

Plants

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“…Inducible promoters limit gene expression spatially and temporally to specific organs at appropriate times for determining the phenotypic traits displayed in response to differences in environmental conditions (Jin et al, 2019;Li et al, 2016). Here, sequence analysis showed that GmINS1 harbors four SNPs between FC-1 and FC-2 in a 2323-bp promoter region and zero in the CDS (Figure 5A).…”

Section: Discussionmentioning

confidence: 99%

“…Gene expression in higher plants is mediated by promoters via interactions between cis‐ regulatory elements and their cognate transcription factors (Bilas et al, 2016; Kohli, 2005; Liu et al, 2014). Inducible promoters limit gene expression spatially and temporally to specific organs at appropriate times for determining the phenotypic traits displayed in response to differences in environmental conditions (Jin et al, 2019; Li et al, 2016). Here, sequence analysis showed that GmINS1 harbors four SNPs between FC‐1 and FC‐2 in a 2323‐bp promoter region and zero in the CDS (Figure 5A).…”

Section: Discussionmentioning

confidence: 99%

The soybean β‐expansin gene GmINS1 contributes to nodule development in response to phosphate starvation

Yang

Zheng

Zhou

et al. 2021

Physiologia Plantarum

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Legume biological nitrogen fixation (BNF) is the most important N source in agricultural ecosystems. Nodule organogenesis from the primordia to the development of mature nodules with the ability to fix N 2 largely determines BNF capacity. However, nodule growth is often limited by low phosphorus (P) availability, while the mechanisms underlying nodule development responses to P deficiency remain largely unknown. In this study, we found that nodule enlargement is severely inhibited by P deficiency, as reflected by the smaller individual nodule size from a soybean core collection in the field. Wide-ranging natural diversity in nodule size was further identified in soybeans reared in low P soils, with the FC-1 genotype outperforming FC-2 in assessments of nodulation under low P conditions. Among β-expansin members, GmINS1 expression is most abundantly enhanced by P deficiency in FC-1 nodules, and its transcript level is further displayed to be tightly associated with nodule enlargement. Four single nucleotide polymorphisms discovered in the GmINS1 promoter distinguished the FC-1 and FC-2 genotypes and accounted for the differential expression levels of GmINS1 responses to P deficiency. GmINS1 overexpression led to increases in nodule size, infected cell abundance, and N 2 fixation capacity and subsequently promoted increases in N and P content, soybean biomass, and yield. Our findings provide a candidate gene for optimizing BNF capacity responses to low P stress in soybean molecular breeding programs.

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“…While few genes CaRLK 17 and 3 from SD3 and LRRXII subfamily were found to possess those genes and may also imply the same function. The presence of Cis -regulatory elements like F/Sbox, W box, TGACG and MYB binding site in promoter regions are involved in the stress-inducible defense gene in Maize [40].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification, Characterization and Expression Analysis of Non-Arginine Aspartate Receptor like kinase gene family underColletotrichum truncatumstress conditions in Hot pepper

Srideepthi

Krishna

Suneetha

et al. 2020

Preprint

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Receptor Like kinases (RLKs) are conserved upstream signaling molecules that regulate several biological processes, including plant development and stress adaptation. Non arginine aspartate (non-RD) an important class of RLKs plays a vital role in disease resistance and apoptosis in plants. In present investigation, a comprehensive Insilco analysis for non-RD Kinase gene family including identification, sequence similarity, phylogeny, chromosomal localization, gene structures, gene duplication analysis, promoter analysis and transcript expression profiles were elucidated. In this study twenty six genes were observed on nine out of twelve chromosomes. All these genes were clustered into seven subfamilies under large monophyletic group termed as Interleukin-1 Receptor-Associated Kinase (IRAK) family. Structural diversity in genomic structure among non-RD kinase gene family were identified and presence of pathogen induced cis regulatory elements like STRE, MYC, MYB,W box were found. Expression profiles of genes involved in providing resistance to anthracnose pathogen Colletotrichum truncatum in hot pepper were analyzed at different infective stages in both resistant and susceptible genotypes. Among twenty six genes, CaRLK1 gene belonging to LRRXII subfamily was up regulated under severe stress after infection in resistant genotype PBC-80. This integrative approach has helped us to identify candidate genes involved in disease resistance which would be helpful in future crop improvement programs.

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Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize

Cited by 16 publications

References 33 publications

Isolation and Functional Characterization of the Promoters of Miltiradiene Synthase Genes, TwTPS27a and TwTPS27b, and Interaction Analysis with the Transcription Factor TwTGA1 from Tripterygium wilfordii

Isolation and Functional Characterization of the Promoters of Miltiradiene Synthase Genes, TwTPS27a and TwTPS27b, and Interaction Analysis with the Transcription Factor TwTGA1 from Tripterygium wilfordii

The soybean β‐expansin gene GmINS1 contributes to nodule development in response to phosphate starvation

Genome-Wide Identification, Characterization and Expression Analysis of Non-Arginine Aspartate Receptor like kinase gene family underColletotrichum truncatumstress conditions in Hot pepper

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