Cloning and functional analysis of three aphid alarm pheromone genes from German chamomile (Matricaria chamomilla L.)

Ling, Chengcheng; Zheng, Lujie; Yu, Xiaorui; Wang, Huanhuan; Wang, Cheng-Xiang; Wu, Haiyan; Zhang, Jie; Yao, Ping; Tai, Yuling; Yang, Yuan

doi:10.1016/j.plantsci.2020.110463

Cited by 13 publications

(8 citation statements)

References 46 publications

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“…Total RNA was extracted from German Chamomile samples using RNAiso Plus (Takara Bio Inc, Dalian, China) and treated with an RNAprep pure plant kit (Tiangen, Beijing, China). The CTAB method was used to extract genomic DNA [ 39 ], which was stored at −20 °C until use. A NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, USA) and agarose gel electrophoresis were used to determine the quality and concentration of the extracted RNA and DNA.…”

Section: Methodsmentioning

confidence: 99%

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Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

Tai

Wang

Yao

et al. 2023

IJMS

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The essential oil of German chamomile (Matricaria recutita L.) is widely used in food, cosmetics, and the pharmaceutical industry. α-Bisabolol is the main active substance in German chamomile. Farnesyl diphosphate synthase (FPS) and α-bisabolol synthase (BBS) are key enzymes related to the α-bisabolol biosynthesis pathway. However, little is known about the α-bisabolol biosynthesis pathway in German chamomile, especially the transcription factors (TFs) related to the regulation of α-bisabolol synthesis. In this study, we identified MrFPS and MrBBS and investigated their functions by prokaryotic expression and expression in hairy root cells of German chamomile. The results suggest that MrFPS is the key enzyme in the production of sesquiterpenoids, and MrBBS catalyzes the reaction that produces α-bisabolol. Subcellular localization analysis showed that both MrFPS and MrBBS proteins were located in the cytosol. The expression levels of both MrFPS and MrBBS were highest in the extension period of ray florets. Furthermore, we cloned and analyzed the promoters of MrFPS and MrBBS. A large number of cis-acting elements related to light responsiveness, hormone response elements, and cis-regulatory elements that serve as putative binding sites for specific TFs in response to various biotic and abiotic stresses were identified. We identified and studied TFs related to MrFPS and MrBBS, including WRKY, AP2, and MYB. Our findings reveal the biosynthesis and regulation of α-bisabolol in German chamomile and provide novel insights for the production of α-bisabolol using synthetic biology methods.

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Section: Methodsmentioning

confidence: 99%

“…To investigate the function of MrFPS and MrBBS , the recombinant plasmids pCAMBIA1302-MrFPS and pCAMBIA1302-MrBBS were transformed into Agrobacterium rhizogenes , and then transfected into German chamomile for cultivation of hairy roots [ 39 ]. One-month-old German chamomile seedlings were excised and wounds were infected with A. rhizogenes.…”

Section: Methodsmentioning

confidence: 99%

Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

Tai

Wang

Yao

et al. 2023

IJMS

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“…Genetic markers associated with α-bisabalool and chamazulene, have been identified, which can be incorporated during breeding. Furthermore, gene-transfer technology helped in the overexpression of sesquiterpene synthase, resulting in high sesquiterpene content [ 22 ]. Similarly, genetic engineering has helped in the production of essential oil with high yields of terpenoids [ 23 ].…”

Section: Chemical Constituentsmentioning

confidence: 99%

A Comprehensive Study of Therapeutic Applications of Chamomile

et al. 2022

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Chamomile has a long history of traditional medicinal uses. The two commonly used varieties with therapeutic applications are German chamomile known as Matricaria chamomilla L. and Roman chamomile or Chamaemelum nobile L. The plant contains many components, namely, flavonoids, terpenoids, and coumarins, which are responsible for its medicinal properties. The review discusses recent developments that help in establishing its role as a therapeutic agent in various areas as an anti-inflammatory, antioxidant, analgesic, antimicrobial, hepatoprotective, anti-allergic, anticancer, and anti-hypertensive agent. Not much is known about its role in the treatment of CNS disorders and metabolic syndromes, which are also discussed. The chemical components responsible for the therapeutic activity and the respective mechanism of action are also elaborated.

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“…In biosynthesis and the regulation of volatile terpenes, the action of aphid alarm pheromones forms the basis for molecular study. qPCR signified high expression of these genes in young flowers and showed higher correlation with amounts of essential oil compounds viz., (E)-β-farnesene, β-elemene and germacrene D [ 106 ]. Mutation breeding with chemical radiation mutagens, mass selection and polyploidization breeding were used as the major conventional breeding techniques for German chamomile improvement.…”

Section: Genetic Improvementmentioning

confidence: 99%

A Comprehensive Review on Biology, Genetic Improvement, Agro and Process Technology of German Chamomile (Matricaria chamomilla L.)

Chauhan

Singh

Kumar

et al. 2021

Plants

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German chamomile (M. chamomilla) is recognized as a star herb due to its medicinal and aromatic properties. This plant is found across a wide range of climatic and soil conditions. Both the flower heads and blue essential oils of German chamomile possess several pharmacological properties of an anti-inflammatory, antimicrobial, antiseptic, antispasmodic and sedative, etc., nature, which makes it a highly sought after herb for use in many pharma and aroma industries. Chamomile tea, prepared from its flower heads, is also a well-known herbal tea for mind and body relaxation. Though it is a high-demand herb, farmers have not adopted this plant for large scale cultivation as a crop, which could improve their livelihood, due to the high cost in flower heads harvesting, loss in over mature and immature flower heads picking during harvesting, unavailability of varieties and agrotechnologies for machine harvesting, a lack of efficient process development of oil extraction and in the lack of improved stable varieties. There are many studies that have reported on the phytochemistry and pharmacological uses of chamomile, which further explore its importance in the medicine industry. Several studies are also present in the literature on its cultivation practices and plant ecology. However, studies on breeding behavior, genetic improvement, varietal development and mechanical harvesting are scarce in German chamomile. Hence, keeping in mind various aspects of farmers’ and researchers’ interest, earlier reports on taxonomy, floral biology, processing of oil extraction, active constituents, uses, agronomy, breeding challenges and opportunities in German chamomile are summarized in this review.

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Cloning and functional analysis of three aphid alarm pheromone genes from German chamomile (Matricaria chamomilla L.)

Cited by 13 publications

References 46 publications

Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

A Comprehensive Study of Therapeutic Applications of Chamomile

A Comprehensive Review on Biology, Genetic Improvement, Agro and Process Technology of German Chamomile (Matricaria chamomilla L.)

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