Cloning and functional characterization of human septin 10, a novel member of septin family cloned from dendritic cells

Sui, Lili; Zhang, Wei‐Ping; Liu, Qiuyan; Chen, Taoyong; Li, Nan; Wan, Tao; Yu, Min; Cao, Xuetao

doi:10.1016/s0006-291x(03)00601-6

Cited by 24 publications

(17 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1d, Sept12 can homodimerize and interact robustly with most mammalian septins, with the exception of the other members of its subgroup (Sept3 and Sept9) and Sept10. The latter has been observed in the nucleus, where it may have a function unique from that described for other septins [Sui et al, 2003]. In addition, this pairwise interaction appears to be dependent upon the ability of septins to coordinate a guanine nucleotide.…”

Section: Resultsmentioning

confidence: 87%

Sept12 is a component of the mammalian sperm tail annulus

Steels

Estey

Froese

et al. 2007

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

Since their essential role in cytokinesis was first shown in yeast, the septins have been described to function in diverse cellular contexts. The members of this unique class of GTPases are capable of binding and hydrolyzing GTP, associating with membranes and oligomerizing into higher order structures. Here we describe Sept12, a novel septin, identified in a yeast two hybrid screen using Sept5 as the bait. Sept12 contains the primary sequence elements of a septin and is capable of interacting with other septins. In addition, Sept12 purifies with bound nucleotide and binds to phosphoinositides, confirming its identity as a septin. RT-PCR and Northern blots reveal that Sept12 mRNA is expressed predominantly in testis, and this is supported by tissue Western blots. In rats, Sept12 protein levels rise upon sexual maturity and the Sept12 protein colocalizes with the annulus in isolated mature spermatozoa. Further, coexpression of Sept12 with Sept4, an essential annulus component, results in complete colocalization of both proteins into robust and highly curved filaments in CHO cells. This study suggests Sept12 may be involved in mammalian fertility.

show abstract

Section: Resultsmentioning

confidence: 87%

Sept12 is a component of the mammalian sperm tail annulus

Steels

Estey

Froese

et al. 2007

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

show abstract

“…SEPT10 was initially identified in dendritic cells, where it is abundantly expressed (Sui et al, 2003). In contrast, SEPT10 is weakly expressed in the spleen, thymus, and peripheral blood leukocytes.…”

Section: Immune Systemmentioning

confidence: 99%

Septin functions in organ system physiology and pathology

Dolat

Spiliotis

2013

Biological Chemistry

170

151

View full text Add to dashboard Cite

Human septins comprise a family of 13 genes that encode for >30 protein isoforms with ubiquitous and tissue-specific expressions. Septins are GTP-binding proteins that assemble into higher-order oligomers and filamentous polymers, which associate with cell membranes and the cytoskeleton. In the last decade, much progress has been made in understanding the biochemical properties and cell biological functions of septins. In parallel, a growing number of studies show that septins play important roles for the development and physiology of specific tissues and organs. Here, we review the expression and function of septins in the cardiovascular, immune, nervous, urinary, digestive, respiratory, endocrine, reproductive, and integumentary organ systems. Furthermore, we discuss how the tissue-specific functions of septins relate to the pathology of human diseases that arise from aberrations in septin expression.

show abstract

“…The voltage-dependent anion channel 2-like protein (VDAC) is an essential player by regulating mitochondrial membrane permeability in cell apoptosis (Tsujimoto and Shimizu, 2002). Septins are cytoskeletal proteins assembling as intracellular filamentous scaffolds involved in diverse cellular biological processes including cell apoptosis (Sui et al, 2003). Proteasomes play a critical role in protein degradation and are involved in cell proliferation, apoptosis and stress responses (Zhang and Wei, 2011).…”

Section: Effects Of Bde 47 On the Proteome Of Mussel Gillsmentioning

confidence: 99%