Cloning and immunobiochemical analyses on recombinant chymopapain allergen Cari p 2 showing pollen-fruit cross-reaction

Bhowmik, Moumita; Sarkar, Moumita Biswas; Sarkar, Rajat Kanti; Dasgupta, Angira; Saha, Sudipto; Jana, Kuladip; Sircar, Gaurab; Bhattacharya, Swati Gupta

doi:10.1016/j.molimm.2021.06.010

Cited by 8 publications

(12 citation statements)

References 32 publications

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“…1135 From melon profilin, 1136 cucumisin, an alkaline serine protease, 1137 and a member of the PR-1 family are identified as allergens. 1138 Papaya contains an allergenic endopolygalacturonase, Cari p 1 1139 and a cysteine protease, Cari p 2, 1140 (Figures 99 and 100, and Table 47).…”

Section: Fruitsmentioning

confidence: 99%

“…The list of the World Health Organization/International Union of Immunological Societies Allergen Nomenclature Sub-Committee lists 28 wheat allergens (www.aller gen.org) (Table 49 and Sensitization to wheat among children was <1% in a systematic review taking into account a number of studies around the world 1140 .…”

Section: -Allergenic Moleculesmentioning

confidence: 99%

“…Papaya contains an allergenic endopolygalacturonase, Cari p 1 1139 and a cysteine protease, Cari p 2, 1140 (Figures 99 and 100, and Table 47).…”

Section: B15 – Fruit and Vegetable Allergymentioning

confidence: 99%

“…Sensitization to wheat among children was <1% in a systematic review taking into account a number of studies around the world 1140 . In a population‐based study among six‐year‐old children in Britain, wheat sensitization rate was 0.4% and most of this was concluded to result from grass sensitization based on the food challenges 1182 .…”

Section: B16 – Wheat and Buckwheat Allergiesmentioning

confidence: 99%

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EAACIMolecular Allergology User's Guide 2.0

Dramburg

Hilger

Santos

et al. 2023

Pediatric Allergy Immunology

121

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Since the discovery of immunoglobulin E (IgE) as a mediator of allergic diseases in 1967, our knowledge about the immunological mechanisms of IgE‐mediated allergies has remarkably increased. In addition to understanding the immune response and clinical symptoms, allergy diagnosis and management depend strongly on the precise identification of the elicitors of the IgE‐mediated allergic reaction. In the past four decades, innovations in bioscience and technology have facilitated the identification and production of well‐defined, highly pure molecules for component‐resolved diagnosis (CRD), allowing a personalized diagnosis and management of the allergic disease for individual patients. The first edition of the “EAACI Molecular Allergology User's Guide” (MAUG) in 2016 rapidly became a key reference for clinicians, scientists, and interested readers with a background in allergology, immunology, biology, and medicine. Nevertheless, the field of molecular allergology is moving fast, and after 6 years, a new EAACI Taskforce was established to provide an updated document. The Molecular Allergology User's Guide 2.0 summarizes state‐of‐the‐art information on allergen molecules, their clinical relevance, and their application in diagnostic algorithms for clinical practice. It is designed for both, clinicians and scientists, guiding health care professionals through the overwhelming list of different allergen molecules available for testing. Further, it provides diagnostic algorithms on the clinical relevance of allergenic molecules and gives an overview of their biology, the basic mechanisms of test formats, and the application of tests to measure allergen exposure.

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Section: Fruitsmentioning

confidence: 99%

Section: -Allergenic Moleculesmentioning

confidence: 99%

“…Papaya contains an allergenic endopolygalacturonase, Cari p 1 1139 and a cysteine protease, Cari p 2, 1140 (Figures 99 and 100, and Table 47).…”

Section: B15 – Fruit and Vegetable Allergymentioning

confidence: 99%

Section: B16 – Wheat and Buckwheat Allergiesmentioning

confidence: 99%

See 2 more Smart Citations

EAACIMolecular Allergology User's Guide 2.0

Dramburg

Hilger

Santos

et al. 2023

Pediatric Allergy Immunology

121

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“…48 No information is available on oral and respiratory sensitisation or elicitation reactions of this endopolygalacturonase. 47 Papaya is both a food and a respiratory allergen (Sarkar et al, 2018;Bhowmik et al, 2021). Allergens present in papaya are chitinase, protease (papain), lysozyme, lipid transfer proteins, and recently, a polygalacturonase (Cari p 1) was identified.…”

Section: Allergenicity 47mentioning

confidence: 99%

Safety evaluation of the food enzyme endo‐polygalacturonase from the genetically modified Aspergillus niger strain EPG

Lambré¹,

Baviera²,

Bolognesi³

et al. 2023

EFS2

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The food enzyme endo-polygalacturonase ((1-4)-a-D-galacturonan glycanohydrolase; EC 3.2.1.15) is produced with the genetically modified Aspergillus niger strain EPG by DSM Food Specialties B.V. The genetic modifications do not give rise to safety concerns. The food enzyme is free from viable cells of the production organism and its DNA. It is intended to be used in fruit and vegetable processing for juice production. The dietary exposure to the food enzyme-total organic solids (TOS) was estimated to be up to 0.122 mg TOS/kg body weight (bw) per day in European populations. Genotoxicity tests did not indicate a safety concern. The systemic toxicity was assessed by means of a repeated dose 90-day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 1,014 mg TOS/kg bw per day, the highest dose tested, which, when compared with the estimated dietary exposure, resulted in a margin of exposure at least 8,311. A search for the similarity of the amino acid sequence of the food enzyme to known allergens was made and 38 matches were found, two of which are food allergens. The Panel considered that, under the intended conditions of use, the risk of allergic reactions upon dietary exposure to this food enzyme cannot be excluded, in particular for individuals sensitised to papaya or maize, but that the risk will not exceed that of consumption of papaya or maize. In addition, oral allergy reactions cannot be excluded in pollen-sensitised individuals. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns, under the intended conditions of use.

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A systematic review of allergen cross-reactivity: Translating basic concepts into clinical relevance

Sharma,

Vitte

2024

Journal of Allergy and Clinical Immunology: Global

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Cloning and immunobiochemical analyses on recombinant chymopapain allergen Cari p 2 showing pollen-fruit cross-reaction

Cited by 8 publications

References 32 publications

EAACIMolecular Allergology User's Guide 2.0

EAACIMolecular Allergology User's Guide 2.0

Safety evaluation of the food enzyme endo‐polygalacturonase from the genetically modified Aspergillus niger strain EPG

A systematic review of allergen cross-reactivity: Translating basic concepts into clinical relevance

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