Cloning and initial characterization of human and mouse Spot 14 genes<sup>1</sup>

Grillasca, Joël-Paul; Gastaldi, Marguerite; Khiri, Hacène; Dace, Alexandra; Peyrol, Nicole; Reynier, Pascal; Torresani, Janine; Planells, Richard

doi:10.1016/s0014-5793(96)01433-0

Cited by 31 publications

(22 citation statements)

References 34 publications

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“…This result was consistent with a recent report of S14 mRNA expression in a human lipoma (46). The weak cytoplasmic staining was unaffected by preincubation (Fig.…”

Section: Expression Of S14 In Breast Cancer Cell Lines and Primarysupporting

confidence: 94%

The “Spot 14” gene resides on the telomeric end of the 11q13 amplicon and is expressed in lipogenic breast cancers: Implications for control of tumor metabolism

Moncur

Park

Memoli

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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Enhanced long chain fatty acid synthesis may occur in breast cancer, where it is necessary for tumor growth and predicts a poor prognosis. ''Spot 14'' (S14) is a carbohydrate-and thyroid hormone-inducible nuclear protein specific to liver, adipose, and lactating mammary tissues that functions to activate genes encoding the enzymes of fatty acid synthesis. Amplification of chromosome region 11q13, where the S14 gene (THRSP) resides, also predicts a poor prognosis in breast tumors. We localized the S14 gene between markers D11S906 and D11S937, at the telomeric end of the amplified region at 11q13, and found that it was amplified and expressed in breast cancer-derived cell lines. Moreover, concordant expression of S14 and a key lipogenic enzyme (acetyl-CoA carboxylase) in a panel of primary breast cancer specimens strongly supported a role for S14 as a determinant of tumor lipid metabolism. S14 expression provides a pathophysiological link between two prognostic indicators in breast cancer: enhanced lipogenesis and 11q13 amplification.

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“…This result was consistent with a recent report of S14 mRNA expression in a human lipoma (46). The weak cytoplasmic staining was unaffected by preincubation (Fig.…”

Section: Expression Of S14 In Breast Cancer Cell Lines and Primarysupporting

confidence: 94%

The “Spot 14” gene resides on the telomeric end of the 11q13 amplicon and is expressed in lipogenic breast cancers: Implications for control of tumor metabolism

Moncur

Park

Memoli

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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“…The 5Ј primer was 5Ј-GGAATTCGCAGCCTCCAT-CACATCCTTAC-3Ј; the underlined sequence is an EcoRI site for subcloning followed by spot 14 exon 1 sequence (43). The 3Ј primer was 5Ј-GGGATCCACCGCCATTTATCTCCTCCCTC-3Ј; the BamHI restriction site for subcloning is underlined and is followed by spot 14 intron sequence (this sequence was kindly provided by Dr. Richard Planells, INSERM, Marseille, France).…”

Section: Methodsmentioning

confidence: 99%

Regulation of the Processing of Glucose-6-phosphate Dehydrogenase mRNA by Nutritional Status

Amir-Ahmady

Salati

2001

Journal of Biological Chemistry

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Expression of glucose-6-phosphate dehydrogenase (G6PD) gene during starvation and refeeding is regulated by a posttranscriptional mechanism occurring in the nucleus. The amount of G6PD mRNA at different stages of processing was measured in RNA isolated from the nuclear matrix fraction of mouse liver. This nuclear fraction contains nascent transcripts and RNA undergoing processing. Using a ribonuclease protection assay with probes that cross an exon-intron boundary in the G6PD transcript, the abundance of mRNAs that contain the intron (unspliced) and without the intron (spliced) was measured. Refeeding resulted in 6-and 8-fold increases in abundance of G6PD unspliced and spliced RNA, respectively, in the nuclear matrix fraction. However, the amount of G6PD unspliced RNA was at most 15% of the amount of spliced RNA. During refeeding, G6PD spliced RNA accumulated at a rate significantly greater than unspliced RNA. Further, the amount of partially spliced RNA exceeded the amount of unspliced RNA indicating that the enhanced accumulation occurs early in processing. Starvation and refeeding did not regulate either the rate of polyadenylation or the length of the poly(A) tail. Thus, the G6PD gene is regulated during refeeding by enhanced efficiency of splicing of its RNA, and this processing protects the mRNA from decay, a novel mechanism for nutritional regulation of gene expression.Glucose-6-phosphate dehydrogenase (G6PD, 1 EC 1.1.1.49) is the rate-limiting enzyme of the pentose phosphate pathway. All cells contain G6PD activity; however, nutritional and hormonal factors only regulate the expression of the enzyme in liver and adipose tissue (1-3). Regulation of G6PD activity in these tissues is because it plays a critical role in the de novo synthesis of fatty acids by providing 50 -75% of the NADPH for the fatty acid synthase reaction (4). Thus, G6PD is a member of the lipogenic enzyme family. The activities of the lipogenic enzymes are coordinately regulated so that flux of substrate through the fatty acid biosynthetic pathway is high when animals consume a diet rich in carbohydrate and flux is decreased by starvation or the addition of polyunsaturated fat to the diet (reviewed in Ref. 5). However, the molecular mechanisms causing these changes in flux vary considerably between enzymes.The enzymes of the fatty acid biosynthetic pathway are regulated at both transcriptional and posttranscriptional steps. Fatty acid synthase (6 -8), acetyl-CoA carboxylase (9, 10), stearoyl-CoA desaturase (11), and ATP-citrate lyase (12) undergo large changes in their transcriptional rates in response to dietary manipulations. Regulation of malic enzyme expression during a chow to fat-free diet transition occurs by changes in mRNA stability in the cytoplasm (13). In addition, cytoplasmic mRNA stability is also involved in the regulation of stearoylCoA desaturase expression by fatty acids in yeast (14) and in adipocytes (15). Posttranscriptional regulation is the primary mechanism involved in the nutritional regulation of G6PD expressi...

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“…For example, the insulin-like growth factor-binding protein 1 (Igfbp1) gene binds both IGFI and II in the plasma, prolongs the half-lives of IGFs and alters their interactions with cell surface receptors (Wood et al, 2005). The protein product thyroid hormone-inducible hepatic protein (THRSP) is similar to the gene product of S14, which is expressed in the liver and adipocytes, particularly in lipomatous modules (Grillasca et al, 1997). These genes have roles in fatty acid metabolism processes and may be considered candidate genes for marker-assisted selection (MAS) in bighead carp.…”

Section: Differential Gene Expression In the Two Groupsmentioning

confidence: 99%

Comparative transcriptomic analyses of two bighead carp ( Hypophthalmichthys nobilis ) groups with different growth rates

Wang

Feng

et al. 2016

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Cloning and initial characterization of human and mouse Spot 14 genes¹

Cited by 31 publications

References 34 publications

The “Spot 14” gene resides on the telomeric end of the 11q13 amplicon and is expressed in lipogenic breast cancers: Implications for control of tumor metabolism

The “Spot 14” gene resides on the telomeric end of the 11q13 amplicon and is expressed in lipogenic breast cancers: Implications for control of tumor metabolism

Regulation of the Processing of Glucose-6-phosphate Dehydrogenase mRNA by Nutritional Status

Comparative transcriptomic analyses of two bighead carp ( Hypophthalmichthys nobilis ) groups with different growth rates

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Cloning and initial characterization of human and mouse Spot 14 genes1

Cited by 31 publications

References 34 publications

The “Spot 14” gene resides on the telomeric end of the 11q13 amplicon and is expressed in lipogenic breast cancers: Implications for control of tumor metabolism

The “Spot 14” gene resides on the telomeric end of the 11q13 amplicon and is expressed in lipogenic breast cancers: Implications for control of tumor metabolism

Regulation of the Processing of Glucose-6-phosphate Dehydrogenase mRNA by Nutritional Status

Comparative transcriptomic analyses of two bighead carp ( Hypophthalmichthys nobilis ) groups with different growth rates

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Cloning and initial characterization of human and mouse Spot 14 genes¹