Cloning and overexpression of transferrin gene from cypermethrin-resistant Culex pipiens pallens

Tan, Wenfeng; Wang, Xiao; Cheng, Peng; Liu, Lijuan; Wang, Haifang; Gong, Maoqing; Quan, Xin; Gao, Honggang; Zhu, Changliang

doi:10.1007/s00436-011-2580-4

Cited by 15 publications

(11 citation statements)

References 41 publications

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“…3 C). There have been no reports of plant secondary chemicals inducing the expression of transferrin, but some have suggested that transferrin is expressed at higher levels in cypermethrin-resistant strains and that this may confer insecticide resistance in C. pipiens pallens (Tan et al ., 2012 ). dsRNA-mediated depletion of HaTrf in second-instar larvae decreased their tolerance to 2-tridecanone (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro experiments have indicated that transferrin-transfected cells also show enhanced cypermethrin resistance compared with null-transfected or plasmid vector-transfected cells, as determined by methyl tritiated thymidine ( 3 H-TdR) incorporation. These results indicate that the expression of transferrin could be induced by the xenobiotic cypermethrin in the resistant strains, and that transferrins might confer insecticide resistance in C. pipiens pallens (Tan et al ., 2012 ; Vezilier et al ., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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A transferrin gene associated with development and 2‐tridecanone tolerance in Helicoverpa armigera

Zhang

Shang

et al. 2014

Insect Molecular Biology

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The full-length cDNA (2320 bp) encoding a putative iron-binding transferrin protein from Helicoverpa armigera was cloned and named HaTrf. The putative HaTrf sequence included 670 amino acids with a molecular mass of approximately 76 kDa. Quantitative PCR results demonstrated that the transcriptional level of HaTrf was significantly higher in the sixth instar and pupa stages as compared with other developmental stages. HaTrf transcripts were more abundant in fat bodies and in the epidermis than in malpighian tubules. Compared with the control, the expression of HaTrf increased dramatically 24 h after treatment with 2-tridecanone. Apparent growth inhibition with a dramatic body weight decrease was observed in larvae fed with HaTrf double-stranded RNA (dsRNA), as compared with those fed with green fluorescent protein dsRNA. RNA interference of HaTrf also significantly increased the susceptibility of larvae to 2-tridecanone. These results indicate the possible involvement of HaTrf in tolerance to plant secondary chemicals.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A transferrin gene associated with development and 2‐tridecanone tolerance in Helicoverpa armigera

Zhang

Shang

et al. 2014

Insect Molecular Biology

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“…Transferrin ( Tf1 - AAEL015458), found to be under expressed in temephos resistant larvae in the current study, also has roles in the innate immune response to arbovirus infection [98, 103] and has previously been reported to be downregulated in CHIKV and DENV infected mosquitoes which may favour viral replication [104]. Transferrin expression has also been related to insecticide resistance in Culex pipiens with increased expression reported in mosquitoes with target-site resistance to pyrethroids and organophosphates, the biggest difference in transferrin expression was observed in adults [105, 106].…”

Section: Discussionmentioning

confidence: 55%

Expansive and diverse phenotypic landscape of field Aedes aegypti larvae with differential susceptibility to temephos: beyond metabolic detoxification

Morgan

Salcedo-Sora

Triana-Chávez

et al. 2021

Preprint

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Arboviruses including dengue, Zika and chikungunya are amongst the most significant public health concerns worldwide and their control relies heavily on the use of insecticides to control the vector mosquito Aedes aegypti. The success of controlling these vector-pathogen systems is threatened by widespread insecticide resistance. The work presented here profiled the gene expression of the larvae from two field populations of Ae. aegypti with differential susceptibility to temephos. The contrasting phenotypes originated from two Colombian urban locations, Bello and Cucuta, that we have previously reported to have distinctive disease incidence, socioeconomics, and climate. The closeness of the geographical origin of the study populations was suspected to be highly influential in the profiling of the gene expression of resistance since the mosquito resistance levels themselves are highly dependent upon environmental variables. We demonstrated that an exclusive field-to-lab (Ae. aegypti reference strain New Orleans) comparison generates an over estimation of differential gene expression (DGE) and that the inclusion of a geographically relevant field control, as used here, yields a more discrete, and likely, more specific set of genes. The composition of the obtained DGE profiles is varied, with commonly reported resistance associated genes such as detoxifying enzymes having only a small representation. We identify cuticle biosynthesis, ion exchange homeostasis, an extensive number of long non-coding RNAs, and chromatin modelling among the specifically and differentially expressed genes in field resistant Ae. aegypti larvae. It was also shown that temephos resistant larvae undertake further gene expression responses when temporarily exposed to this insecticide. The results from the sampling triangulation approach undertaken here contributes a discrete DGE profiling with reduced noise that permitted the observation of a greater gene diversity. This deeper gene granularity significantly increases the number of potential targets for the control of insecticide resistant mosquitoes and widens our knowledge base on the complex phenotypic network of the Ae. aegypti mosquito responses to insecticides.

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“…Similarly, searches of the ten most highly expressed genes from these previous studies with our data found that none of these previously identified genes were overexpressed in our resistant strains. Candidate genes previously associated with pyrethroid resistance in other species of mosquitoes include transferrin [ 82 ], iron responsive element binding protein [ 83 ], glycogen branching enzyme [ 84 ], chymotrypsin [ 82 ] and G-protein coupled receptor [ 85 ]. None of these genes were overexpressed in our resistant strains, leading us to conclude that they do not play a role in pyrethroid resistance in CKR or SP.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and proteomic analysis of pyrethroid resistance in the CKR strain of Aedes aegypti

et al. 2021

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Aedes aegypti is an important vector of human viral diseases. This mosquito is distributed globally and thrives in urban environments, making it a serious risk to human health. Pyrethroid insecticides have been the mainstay for control of adult A. aegypti for decades, but resistance has evolved, making control problematic in some areas. One major mechanism of pyrethroid resistance is detoxification by cytochrome P450 monooxygenases (CYPs), commonly associated with the overexpression of one or more CYPs. Unfortunately, the molecular basis underlying this mechanism remains unknown. We used a combination of RNA-seq and proteomic analysis to evaluate the molecular basis of pyrethroid resistance in the highly resistant CKR strain of A. aegypti. The CKR strain has the resistance mechanisms from the well-studied Singapore (SP) strain introgressed into the susceptible Rockefeller (ROCK) strain genome. The RNA-seq and proteomics data were complimentary; each offering insights that the other technique did not provide. However, transcriptomic results did not quantitatively mirror results of the proteomics. There were 10 CYPs which had increased expression of both transcripts and proteins. These CYPs appeared to be largely trans-regulated, except for some CYPs for which we could not rule out gene duplication. We identified 65 genes and lncRNAs as potentially being responsible for elevating the expression of CYPs in CKR. Resistance was associated with multiple loci on chromosome 1 and at least one locus on chromosome 3. We also identified five CYPs that were overexpressed only as proteins, suggesting that stabilization of CYP proteins could be a mechanism of resistance. Future studies to increase the resolution of the resistance loci, and to examine the candidate genes and lncRNAs identified here will greatly enhance our understanding of CYP-mediated resistance in A. aegypti.

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Cloning and overexpression of transferrin gene from cypermethrin-resistant Culex pipiens pallens

Cited by 15 publications

References 41 publications

A transferrin gene associated with development and 2‐tridecanone tolerance in Helicoverpa armigera

A transferrin gene associated with development and 2‐tridecanone tolerance in Helicoverpa armigera

Expansive and diverse phenotypic landscape of field Aedes aegypti larvae with differential susceptibility to temephos: beyond metabolic detoxification

Transcriptomic and proteomic analysis of pyrethroid resistance in the CKR strain of Aedes aegypti

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