Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus)

Du, Fukuan; Su, Jianguo; Huang, Rong; Liao, Lanjie; Zhang, Zhu; Wang, Yaping

doi:10.1016/j.fsi.2013.03.352

Cited by 21 publications

(14 citation statements)

References 33 publications

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“…Besides classical PRRs network and ISGs, some other genes are also participating in immune defense against GCRV, such as GCRV receptor junction adhesion molecule A (JAM-A) [ 188 ], voltage-dependent anion-selective channel proteins (VDACs) [ 189 ], ubiquitination pathway-related Nedd4 binding protein 1 (N4BP1) [ 190 ], lipopolysaccharide-induced TNF- α factor (LITAF) [ 191 ], T-bet, and GATA-3 [ 192 ].…”

Section: Innate Antiviral Immunitymentioning

confidence: 99%

Insights into the Antiviral Immunity against Grass Carp (Ctenopharyngodon idella) Reovirus (GCRV) in Grass Carp

Rao

2015

Journal of Immunology Research

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191

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Global fish production from aquaculture has rapidly grown over the past decades, and grass carp shares the largest portion. However, hemorrhagic disease caused by grass carp reovirus (GCRV) results in tremendous loss of grass carp (Ctenopharyngodon idella) industry. During the past years, development of molecular biology and cellular biology technologies has promoted significant advances in the understanding of the pathogen and the immune system. Immunoprophylaxis based on stimulation of the immune system of fish has also got some achievements. In this review, authors summarize the recent progresses in basic researches on GCRV; viral nucleic acid sensors, high-mobility group box proteins (HMGBs); pattern recognition receptors (PRRs), Toll-like receptors (TLRs) and retinoic acid inducible gene I- (RIG-I-) like receptors (RLRs); antiviral immune responses induced by PRRs-mediated signaling cascades of type I interferon (IFN-I) and IFN-stimulated genes (ISGs) activation. The present review also notices the potential applications of molecule genetic markers. Additionally, authors discuss the current preventive and therapeutic strategies (vaccines, RNAi, and prevention medicine) and highlight the importance of innate immunity in long term control for grass carp hemorrhagic disease.

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Section: Innate Antiviral Immunitymentioning

confidence: 99%

Insights into the Antiviral Immunity against Grass Carp (Ctenopharyngodon idella) Reovirus (GCRV) in Grass Carp

Rao

2015

Journal of Immunology Research

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191

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“…The grass carp ( Ctenopharyngodon idella ), with a long breeding history, is one of the most economical herbivorous freshwater fish species and, is widely distributed in Asia [ 1 ]. Due to its great growth performance, rich nutrient content, low cost of breeding and delicious meat, grass carp has become a dominant commercially farmed species, accounting for over 20% of freshwater aquaculture production in China [ 2 ]. Despite their excellent growth traits and large-scale breeding mode, grass carp are susceptible to various diseases.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila

Dang

Shen

et al. 2016

PLoS ONE

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The grass carp (Ctenopharyngodon idella) is an important commercial farmed herbivorous fish species in China, but is susceptible to Aeromonas hydrophila infections. In the present study, we performed de novo RNA-Seq sequencing of spleen tissue from specimens of a disease-resistant family, which were given intra-peritoneal injections containing PBS with or without a dose of A. hydrophila. The fish were sampled from the control group at 0 h, and from the experimental group at 4, 8, 12, 24, 48 and 72 h. 122.18 million clean reads were obtained from the normalized cDNA libraries; these were assembled into 425,260 contigs and then 191,795 transcripts. Of those, 52,668 transcripts were annotated with the NCBI Nr database, and 41,347 of the annotated transcripts were assigned into 90 functional groups. 20,569 unigenes were classified into six main categories, including 38 secondary KEGG pathways. 2,992 unigenes were used in the analysis of differentially expressed genes (DEGs). 89 of the putative DEGs were related to the immune system and 41 of them were involved in the complement and coagulation cascades pathway. This study provides insights into the complement and complement-related pathways involved in innate immunity, through expression profile analysis of the genomic resources in C. idella. We conclude that complement and complement-related genes play important roles during defense against A. hydrophila infection. The immune response is activated at 4 h after the bacterial injections, indicating that the complement pathways are activated at the early stage of bacterial infection. The study has improved our understanding of the immune response mechanisms in C. idella to bacterial pathogens.

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“…Grass carp, the most important freshwater fish species, supplied nearly 20% of the total freshwater aquaculture production in China (Du, Su, Huang, Liao, Zhu & Wang 2013). In juvenile grass carp, Liang et al (2014) suggested that manganese requirement was 19.5 mg kg À1 diet.…”

Section: Introductionmentioning

confidence: 99%

Growth, digestive and absorptive abilities and antioxidative capacity in the hepatopancreas and intestine of young grass carp (Ctenopharyngodon idellusVal.) fed graded levels of dietary manganese

et al. 2014

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gain (PWG) and feed intake were the poorest in fish fed the basal diet (P < 0.05). The activities of trypsin, lipase and alkaline phosphatase in the intestine were significantly enhanced with dietary manganese level at 13.48 mg kg À1 diet (P < 0.05). Additionally, in the hepatopancreas and intestine, the protein carbonyl and malondialdehyde contents were the lowest in fish fed the diet with dietary manganese level at 13.48 mg kg À1 diet (P < 0.05), while the anti-hydroxyl radical capacities, manganese superoxide dismutase (MnSOD), glutathione peroxidase and glutathione-S-transferase activities were significantly enhanced with dietary manganese level at 13.48 mg kg À1 diet (P < 0.05). Moreover, the catalase activity and glutathione content in the intestine were the highest in fish fed the diet with dietary manganese level at 18.24 mg kg À1 diet (P < 0.05). These results indicated that optimum dietary manganese promoted growth, enhanced the digestive and absorptive abilities, and improved the antioxidative capacity in young grass carp. Based on the quadratic regression analysis for PWG and intestinal MnSOD activity, the manganese requirements for young grass carp with the initial body weight of 264 g were 16.91 and 18.21 mg kg À1 diet respectively.

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Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus)

Cited by 21 publications

References 33 publications

Insights into the Antiviral Immunity against Grass Carp (Ctenopharyngodon idella) Reovirus (GCRV) in Grass Carp

Insights into the Antiviral Immunity against Grass Carp (Ctenopharyngodon idella) Reovirus (GCRV) in Grass Carp

Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila

Growth, digestive and absorptive abilities and antioxidative capacity in the hepatopancreas and intestine of young grass carp (Ctenopharyngodon idellusVal.) fed graded levels of dietary manganese

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