Cloning and Sequence Analysis of Two cDNA Encoding Invertase Inhibitors from Cassava (&lt;i&gt;Manihot esculenta Crantz&lt;/i&gt;)

Geng, Meng Ting; Yao, Yuan; Wu, Xiao; Yi, Min; Fu, Shao Ping; Hu, Xueli; Guo, Jian Chun

doi:10.4028/www.scientific.net/amr.726-731.4326

Cited by 2 publications

(2 citation statements)

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“…However, invertase activity can be regulated by post-transcriptional level [43], Such as, in potato tuber, the vacuolar invertase (StvacINV1) was regulated by invertase inhibitor (StInvInh2) during cold-induced sweetening [44]; the vacuolar invertase inhibitor (SolyVIF) of tomato inhibited the vacuolar invertase (TIV-1) and played an important role during tomato plant development [45]. In cassava, we have cloned two speculated vacuolar invertase inhibitors ( MeINH1 and MeINH2 ) [46]. How the cassava vacuolar invertases and inhibitors ( MeVINVs and MeINHs ) regulate the sucrose metabolism during tuber root development will further investigated.…”

Section: Resultsmentioning

confidence: 99%

Cloning, 3D Modeling and Expression Analysis of Three Vacuolar Invertase Genes from Cassava (Manihot Esculenta Crantz)

Yao

Geng

et al. 2014

Molecules

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Vacuolar invertase is one of the key enzymes in sucrose metabolism that irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose in plants. In this research, three vacuolar invertase genes, named MeVINV1-3, and with 653, 660 and 639 amino acids, respectively, were cloned from cassava. The motifs of NDPNG (β-fructosidase motif), RDP and WECVD, which are conserved and essential for catalytic activity in the vacuolar invertase family, were found in MeVINV1 and MeVINV2. Meanwhile, in MeVINV3, instead of NDPNG we found the motif NGPDG, in which the three amino acids GPD are different from those in other vacuolar invertases (DPN) that might result in MeVINV3 being an inactivated protein. The N-terminal leader sequence of MeVINVs contains a signal anchor, which is associated with the sorting of vacuolar invertase to vacuole. The overall predicted 3D structure of the MeVINVs consists of a five bladed β-propeller module at N-terminus domain, and forms a β-sandwich module at the C-terminus domain. The active site of the protein is situated in the β-propeller module. MeVINVs are classified in two subfamilies, α and β groups, in which α group members of MeVINV1 and 2 are highly expressed in reproductive organs and tuber roots (considered as OPEN ACCESSMolecules 2014, 19 6229 sink organs), while β group members of MeVINV3 are highly expressed in leaves (source organs). All MeVINVs are highly expressed in leaves, while only MeVINV1 and 2 are highly expressed in tubers at cassava tuber maturity stage. Thus, MeVINV1 and 2 play an important role in sucrose unloading and starch accumulation, as well in buffering the pools of sucrose, hexoses and sugar phosphates in leaves, specifically at later stages of plant development.

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Section: Resultsmentioning

confidence: 99%

Cloning, 3D Modeling and Expression Analysis of Three Vacuolar Invertase Genes from Cassava (Manihot Esculenta Crantz)

Yao

Geng

et al. 2014

Molecules

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“…In cassava, genome-wide identification, expression, and activity analysis of the INV gene family as well as INV inhibitor was reported recently (Geng et al, 2013; Yao et al, 2014, 2015). However, their function in regulating source-sink carbon partitioning and effects on plant phenotype development in cassava have not been studied.…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink

Yan

et al. 2019

Front. Plant Sci.

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Storage roots are the main sink for photo-assimilate accumulation and reflect cassava yield and productivity. Regulation of sugar partitioning from leaves to storage roots has not been elucidated. Cell wall invertases are involved in the hydrolysis of sugar during phloem unloading of vascular plants to control plant development and sink strength but have rarely been studied in root crops like cassava. MeCWINV3 encodes a typical cell wall invertase in cassava and is mainly expressed in vascular bundles. The gene is highly expressed in leaves, especially mature leaves, in response to diurnal rhythm. When MeCWINV3 was overexpressed in cassava, sugar export from leaves to storage roots was largely inhibited and sucrose hydrolysis in leaves was accelerated, leading to increased transient starch accumulation by blocking starch degradation and reduced overall plant growth. The progress of leaf senescence was promoted in the MeCWINV3 over-expressed cassava plants with increased expression of senescence-related genes. Storage root development was also delayed because of dramatically reduced sugar allocation from leaves. As a result, the transcriptional expression of starch biosynthetic genes such as small subunit ADP-glucose pyrophosphorylase , granule-bound starch synthase I , and starch branching enzyme I was reduced in accordance with insufficient sugar supply in the storage roots of the transgenic plants. These results show that MeCWINV3 regulates sugar allocation from source to sink and maintains sugar balance in cassava, thus affecting yield of cassava storage roots.

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Cloning and Sequence Analysis of Two cDNA Encoding Invertase Inhibitors from Cassava (<i>Manihot esculenta Crantz</i>)

Cited by 2 publications

References 8 publications

Cloning, 3D Modeling and Expression Analysis of Three Vacuolar Invertase Genes from Cassava (Manihot Esculenta Crantz)

Cloning, 3D Modeling and Expression Analysis of Three Vacuolar Invertase Genes from Cassava (Manihot Esculenta Crantz)

Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink

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